Molecular characterization of a Xylanase-producing fungus isolated from fouled soil

Sakthiselvan, Punniavan; Naveena, Balakrishnan; Partha, Nagarajan

doi:10.1590/s1517-83822014000400020

Cited by 22 publications

(12 citation statements)

References 38 publications

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“…For cellulases, molecular weight sizes of 25-50 kDa were reported in Trichoderma reesei and Phanerochaete (Nayebyazdi et al, 2012), 20-45 kDa in Aspergillus niger (Ncube et al, 2012), 25-100 kDa in endophytes (Elisashvili et al, 2015) and 30-250 kDa in most fungi (Kuhad et al, 2011;Li et al, 2011;Liming and Xueliang, 2004;Santa-Rosa et al, 2018;Yasmin et al, 2013;Ritter et al, 2013;Zhang and Zhang, 2013). For xylanases, molecular weight sizes of 20-50 kDa were reported in endophytes (Polizeli et al, 2005), 18-52 in Aspergillus aculeatus (Fujimoto et al, 1995), 29 kDa in Hypocrea lixii (Sakthiselvan et al, 2014), 19 kDa in Aspergillus fumigatus (Silva et al, 1999) and 45-70 kDa in Neocallimastix frontalis (de Segura and Fevre, 1993).…”

Section: Most Studies Nowadays Have Focused On Cellulases Andmentioning

confidence: 99%

Diversity of cellulase- and xylanase-producing filamentous fungi from termite mounds

Sibanda¹,

Ruzvidzo²,

Ncube³

et al. 2019

J. Yeast Fungal Res.

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Cellulases and xylanases are enzymes of industrial significance, particularly in the pulp, paper, textile, and animal feed industries. Moreover, their utilization in the food industry, among them, bakery, brewery, winery and fruit and vegetable juice production, cannot be underestimated. One of the potential sources of enzymes is the filamentous fungi, and hence bio-prospecting of this specific group of microorganisms with the highest levels of cellulase and xylanase secretions is being continuously undertaken. The specific aim of this study was to isolate and characterize cellulase-and xylanaseproducing filamentous fungi from termite mounds. Termite mounds have long been established as very good sources of filamentous fungi with the ability to secrete high levels of lignocellulolytic enzymes, and hence an ideal target for the bio-prospecting of cellulases and xylanases. In this study, various groups of filamentous fungi were isolated through enrichment and repeated sub-culturing. This was followed by screening using the Congo red plate-based assay. Cellulase and xylanase activities during the solid-state fermentation of wheat bran were detected and analyzed through spectrophotometry via the 3,5-dinitrosalicylic acid detection system for reducing sugars. The obtained fungal isolates were then finally characterized through zymography, reaction kinetics and morphological studies. Overall, a total of eight different groups of fungi, capable of decomposing cellulose and hemicellulose, were isolated, and their tentative identities established as Fusarium, Didymostible, Penicillium, Phytophthora, Oedocephalum, Aspergillus, Monosporascus and Acremonium. Taken together, findings of this study conceivably showed that termite mounds are a good source of filamentous fungi that in turn are also a good source of cellulases and xylanases that arguably, can be recommended for use in industrial and commercial settings.

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Section: Most Studies Nowadays Have Focused On Cellulases Andmentioning

confidence: 99%

Diversity of cellulase- and xylanase-producing filamentous fungi from termite mounds

Sibanda¹,

Ruzvidzo²,

Ncube³

et al. 2019

J. Yeast Fungal Res.

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“…The extent of germination of most spores is influenced by media components and environmental factors such as temperature, pH, relative humidity, etc. (Sakthiselvan et al 2014;Colin et al 2013). In the present work, the experiment was carried out with optimized seed media which was developed in the laboratory and the effect of pH and temperature on germination of spore with respect to time was critically evaluated.…”

Section: Discussionmentioning

confidence: 99%

Identification of markers at various stages of batch fermentation and improved production of xylanase using Aspergillus niger (KP874102.1)

et al. 2018

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Improved xylanase production was carried out through optimization of environmental stresses during spore preservation, seed cultivation and batch fermentation and identifies the markers at various stages. The maximum spore size (radius 6.5 µm) of was noticed after 28 days of spore preservation. During seed cultivation, the hypha formed alongside of germination tube (length 196.8 µm) was noticed only at pH-7 after 18 h of incubation at 28 °C. Therefore, pH-7 and 28 °C were considered as optimum during seed cultivation. In this stage, the final pH of the medium was found to be 6.2 which can be used as marker for completion of seed culture. The production media was optimized through Taguchi methodology. The maximum xylanase production was found to be 1575.93 U. The optimum concentration for media components was found to be xylan from beechwood of 3 g/l, potassium nitrate of 10 g/l, magnesium sulphate of 5 g/l, di-potassium hydrogen phosphate of 50 mM, calcium carbonate of 2 g/l, 1000× of trace element (1 ml) and sodium chloride of 5 g/l. It is evident that improved production of xylanase can be possible through optimization of environmental stresses during spore preservation, seed cultivation and batch fermentation and can be intensified through identification of markers at various stages of fermentation process.

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“…Por otra parte, los tratamientos de T. harzianum (N) y Azotobacter sp. (B) inhibieron el crecimiento de las mismas, sin embargo, Sakthiselvan et al (2014) han sugerido que T. harzianum (N) puede favorecer el crecimiento vegetal, y cuando se mezcla con carbón de bajo rango (CBR) generan un efecto positivo sobre algunas propiedades químicas del suelo aumentando la solubilización de contaminantes y la capacidad de absorción de los mismos.…”

Section: Tratamiento Planta Germinación + (%) Ig Con Relación Al Contunclassified

Mecanismo de acción de cinco microorganismos promotores de crecimiento vegetal

2017

rcia

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El uso indiscriminado de agroquímicos para aumentar la productividad de los cultivos, es una práctica no amigable con el ambiente. Como estrategia para desplazar el uso de químicos agrícolas, se aplica la bioinoculación de microorganismos promotores de crecimiento vegetal. En este contexto, el objetivo fue evaluar el efecto promotor del crecimiento vegetal de cinco cepas de microorganismos sobre seis especies de plantas. Se seleccionó un hongo (Trichoderma harzianum) y un grupo de bacterias (Enterobacter aerogenes, Azotobacter sp., Bacillus mycoides y Microbacterium sp.) para evaluar su efecto sobre la germinación, crecimiento y asimilación de plantas de yuca Manihot esculenta, arroz Oryza sativa, girasol Helianthus annuus L., arveja Pisum sativum L., lechuga Lactuca sativa L. y papaya Carica papaya L. Para el montaje del bioensayo se dispuso de 18 macetas para cada planta. Cada tratamiento se realizó por triplicado incluyendo el control. Se elaboró el inoculo sembrando una alícuota de cada microorganismos en 200mL de caldo nutritivo, y de este se agregaron 20mL a cada maceta por tratamiento. Se evaluaron las siguientes variables: número y longitud de raíces, hojas y tallo, área foliar y biomasa raíz y tallo. Los biopreparados a partir de Microbacterium sp. y B. mycoides, aceleraron la germinación de las plantas sin importar la especie. Los tratamientos de Azotobacter sp., T. harzianum y E. aerogenes no fueron efectivos para las plantas de yuca y arroz. La bioinoculación de Microbacterium sp., B. mycoides y E. aerogenes favoreció el crecimiento, desarrollo y rendimiento de las plantas de arroz y arveja.

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Molecular characterization of a Xylanase-producing fungus isolated from fouled soil

Cited by 22 publications

References 38 publications

Diversity of cellulase- and xylanase-producing filamentous fungi from termite mounds

Diversity of cellulase- and xylanase-producing filamentous fungi from termite mounds

Identification of markers at various stages of batch fermentation and improved production of xylanase using Aspergillus niger (KP874102.1)

Mecanismo de acción de cinco microorganismos promotores de crecimiento vegetal

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