Molecular Characterization of Advanced Mutants for Early Detection of High β-carotene Concetration in Pepper Breeding Programms

Petrov, Veselin; Denev, Iliya; Draganov, M.; Timin, O.Yu.; Panchev, I.; Tomlekova, Nasya

doi:10.7546/cr-2013-66-2-13101331-20

Cited by 8 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lutein results were evaluated statistically and the differences between the results in both mutant lines and control were found to be insignificant (Table 1). Petrov et al (2013), Tomlekova et al (2009b) and Tomlekova et al (2021) obtained the same results on mutant pepper lines. On tomato mutant lines that were examined for b-carotene capacity, results showed the induced mutant lines have higher b-carotene content than control (Tomlekova et al, 2014).…”

Section: Carotenoid Analysissupporting

confidence: 63%

“…Though not widely understood by consumers, induced mutation provides an opportunity for plant breeders to create new varieties, including vegetable crops (lettuce, tomato, pepper, green bean, eggplant), field crops (wheat, barley, chickpea, rice, cotton), fruit crops (peach, cherry, lemon, mandarin, apple, banana), and ornamental crops (chrysanthemum, tulip, carnation). Mutants producing a high yield or increased vitamin C (ascorbic acid) or antioxidant capacity have been produced in bell and green pepper (Tomlekova et al, 2009a;Petrov et al, 2013) and tomato (Levin et al, 2006;Cebolla-Cornejo et al, 2013;Kornienko et al, 2014;Tomlekova et al, 2014) breeding programs. The economic value of a new variety can be assessed based on several parameters, such as yield, savings (e.g., water), and nutritional content.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Gamma rays induced enhancement in the phytonutrient capacities of tomato (Solanum Lycopersicum L.)

et al. 2023

View full text Add to dashboard Cite

One of the most important problems for the field-grown Ayaş tomato (Solanum lycopersicum Ayas population), which is preferred for consumption in Central Anatolia because of its aromatic taste, is that the shelf life is short because of insufficient fruit firmness. Therefore, a study was initiated to develop high-quality lines and variety candidates through mutation breeding in the current Ayaş population. In this study, the effective mutation dose (EMD50) was found to be 150 Gy for seeds using a Cobalt-60 gamma ray source. The main mutant population was generated by applying EMD50. During the study, mutant lines selected by following the classical mutation breeding stages were evaluated in terms of yield and quality traits (antioxidant content, total soluble solid amount, fruit firmness, Brix, etc.) starting from the M4 stage. One of our aims was to determine and improve the phytonutrient content of field-grown tomatoes. The fruits of selected lines at the M4 stage were extracted by liquid-liquid partition and accelerated solvent extraction techniques and analyzed by High Performance Liquid Chromatography (HPLC) in this study. It was found that 28 of the 29 mutant lines were significantly different from the control and mutant lines. Mutant lines 9-22, 8-90, 8-135, and 8-127 were determined to be the most promising for commercialization.

show abstract

Section: Carotenoid Analysissupporting

confidence: 63%

mentioning

confidence: 99%

Gamma rays induced enhancement in the phytonutrient capacities of tomato (Solanum Lycopersicum L.)

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Preliminary data mining for similar phenotypes had pointed to CrtZ as the most probable target gene [43,44]. The analysis of CrtZ expression by RT-PCR revealed that mRNA for CrtZ ch06 was presented in both genotypes, while CrtZ ch03 was expressed only in the initial and was absent in the mutant.…”

Section: Discussionmentioning

confidence: 99%

Mutation Associated with Orange Fruit Color Increases Concentrations of β-Carotene in a Sweet Pepper Variety (Capsicum annuum L.)

Tomlekova

Spasova-Apostolova²,

Pantchev

et al. 2021

Foods

Self Cite

View full text Add to dashboard Cite

Pepper is the second most important vegetable crop in Bulgarian agriculture and has become the subject of extensive breeding programs that frequently employ induced mutagenesis. The success of breeding programs can be enhanced by the efficient and integral application of different biochemical and molecular methods to characterize specific mutant alleles. On the other hand, identifying new cost-effective methods is important under a limited-resources environment. In this paper we compare the levels of five health-related carotenoid compounds of fruits (α-carotene, β-carotene, lutein, β-cryptoxanthin, zeaxanthin) between a mutant variety Oranzheva kapia (possessing high ß-carotene concentration) and a corresponding initial pepper variety Pazardzhishka kapia 794. Both varieties are intended for fresh consumption. Pepper is a major natural source of β-carotene. It was observed that fruit at both commercial and botanical maturity from mutant variety had greater α-carotene and β-carotene concentrations to the initial variety (7.49 and 1.94 times higher, respectively) meaning that the mutant was superior in fruit quality to the initial genotype. Two hydroxylase enzymes, converting α- and β-carotene to lutein and zeaxanthin, respectively, are known to exist in pepper and are encoded by two genes on chromosomes 3 and 6-CrtZchr03 and CrtZchr06. The molecular characterization of the mutant variety through locus-specific Polymerase chain reaction amplification, gene cloning and sequencing as well as expression was performed. Our results suggest that the increased ß-carotene accumulation in the mutant variety Oranzheva kapia results from a biosynthetic pathway breakdown due to deletion of CrtZchr03 gene.

show abstract

“…An additional orange-fruited induced mutant (of) with elevated levels of b-carotene accumulation was developed via X-ray mutagenesis of dry seeds of a red-fruited genotype. This mutation was later termed the ''orange-fruited'' trait and was found to be in the 3#-terminal region of CrtZ (Daskalov, 1986;Daskalov and Poulos, 1994;Petrov et al, 2013). This of mutation was transferred into several cultivars that subsequently displayed enhanced b-carotene levels at maturity.…”

Section: Carotenoids and Fruit Colormentioning

confidence: 99%

Capsicum—An Abbreviated Compendium

Jarret¹,

Barboza²,

Batista³

et al. 2019

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Pepper (Capsicum L.) is a major vegetable and spice crop worldwide. Global production of both fresh and dried fruit continues to increase steadily in terms of area harvested and yield. Various topics are addressed in this review, including recent additions to and clarification of Capsicum taxonomy, genetic resources of Capsicum, cytogenetic studies, the current status of our understanding of the mechanisms affecting the biosynthesis of capsaicinoids, the use of gene mutations to elucidate carotenoid biosynthetic pathways and their regulation, and recent advances in whole-genome sequencing and assembly.

show abstract

Molecular Characterization of Advanced Mutants for Early Detection of High β-carotene Concetration in Pepper Breeding Programms

Cited by 8 publications

References 5 publications

Gamma rays induced enhancement in the phytonutrient capacities of tomato (Solanum Lycopersicum L.)

Gamma rays induced enhancement in the phytonutrient capacities of tomato (Solanum Lycopersicum L.)

Mutation Associated with Orange Fruit Color Increases Concentrations of β-Carotene in a Sweet Pepper Variety (Capsicum annuum L.)

Capsicum—An Abbreviated Compendium

Contact Info

Product

Resources

About