Molecular characterization of African orthobunyaviruses

Yandoko, E. Nakouné; Gribaldo, Simonetta; Finance, C.; Faou, Alain Le; Rihn, Bertrand

doi:10.1099/vir.0.82643-0

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…Unlike for BWAV and PGAV, the relative position identified by our analysis for NDV was not consistent with a previous report examining the S segment sequence of NDV (strain ArB16055) [21]. This previous study had suggested a close genetic relationship to Bunyamwera virus, an observation that was apparently at odds with serological evidence assigning NDV to a distinct serogroup [19].…”

Section: Discussioncontrasting

confidence: 96%

“…Together with repeated isolations from mosquito pools [17], [18], [20] these findings indicate that, as with many of the African orthobunyaviruses, NDV virus might also be much more prevalent than is currently appreciated. Intriguingly, the limited sequencing data previously available for this virus suggests that Nyando virus (strain ArB16055; GenBank Accession AM709781) is closely related to Bunyamwera virus [21], a finding that appears to be at odds with the lack of serological cross-reactivity between these viruses. Indeed Nyando virus is classified into a distinct serogroup (Nyando serogroup), of which it is presently the only member [22].…”

Section: Introductionmentioning

confidence: 98%

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Molecular Characterization of Human Pathogenic Bunyaviruses of the Nyando and Bwamba/Pongola Virus Groups Leads to the Genetic Identification of Mojuí dos Campos and Kaeng Khoi Virus

et al. 2014

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BackgroundHuman infection with Bwamba virus (BWAV) and the closely related Pongola virus (PGAV), as well as Nyando virus (NDV), are important causes of febrile illness in Africa. However, despite seroprevalence studies that indicate high rates of infection in many countries, these viruses remain relatively unknown and unstudied. In addition, a number of unclassified bunyaviruses have been isolated over the years often with uncertain relationships to human disease.Methodology/Principal FindingsIn order to better understand the genetic and evolutionary relationships among orthobunyaviruses associated with human disease, we have sequenced the complete genomes for all 3 segments of multiple strains of BWAV (n = 2), PGAV (n = 2) and NDV (n = 4), as well as the previously unclassified Mojuí dos Campos (MDCV) and Kaeng Khoi viruses (KKV). Based on phylogenetic analysis, we show that these viruses populate 2 distinct branches, one made up of BWAV and PGAV and the other composed of NDV, MDCV and KKV. Interestingly, the NDV strains analyzed form two distinct clades which differed by >10% on the amino acid level across all protein products. In addition, the assignment of two bat-associated bunyaviruses into the NDV group, which is clearly associated with mosquito-borne infection, led us to analyze the ability of these different viruses to grow in bat (RE05 and Tb 1 Lu) and mosquito (C6/36) cell lines, and indeed all the viruses tested were capable of efficient growth in these cell types.Conclusions/SignificanceOn the basis of our analyses, it is proposed to reclassify the NDV strains ERET147 and YM176-66 as a new virus species. Further, our analysis definitively identifies the previously unclassified bunyaviruses MDCV and KKV as distinct species within the NDV group and suggests that these viruses may have a broader host range than is currently appreciated.

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Section: Discussioncontrasting

confidence: 96%

Section: Introductionmentioning

confidence: 98%

Molecular Characterization of Human Pathogenic Bunyaviruses of the Nyando and Bwamba/Pongola Virus Groups Leads to the Genetic Identification of Mojuí dos Campos and Kaeng Khoi Virus

et al. 2014

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“…mosquitoes collected in the Semliki Forest in Uganda [1]. Based on detection of antibodies to BUNV in human sera and isolations of BUNV from patients suffering febrile illness, the virus is widely distributed in several regions of sub-Saharan Africa [2]–[4]. BUNV is maintained in nature by a propagative cycle involving blood-feeding mosquitoes and susceptible vertebrate hosts, probably small rodents [5].…”

Section: Introductionmentioning

confidence: 99%

Role of Bunyamwera Orthobunyavirus NSs Protein in Infection of Mosquito Cells

2012

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BackgroundBunyamwera orthobunyavirus is both the prototype and study model of the Bunyaviridae family. The viral NSs protein seems to contribute to the different outcomes of infection in mammalian and mosquito cell lines. However, only limited information is available on the growth of Bunyamwera virus in cultured mosquito cells other than the Aedes albopictus C6/36 line.Methodology and Principal FindingsTo determine potential functions of the NSs protein in mosquito cells, replication of wild-type virus and a recombinant NSs deletion mutant was compared in Ae. albopictus C6/36, C7-10 and U4.4 cells, and in Ae. aegypti Ae cells by monitoring N protein production and virus yields at various times post infection. Both viruses established persistent infections, with the exception of NSs deletion mutant in U4.4 cells. The NSs protein was nonessential for growth in C6/36 and C7-10 cells, but was important for productive replication in U4.4 and Ae cells. Fluorescence microscopy studies using recombinant viruses expressing green fluorescent protein allowed observation of three stages of infection, early, acute and late, during which infected cells underwent morphological changes. In the absence of NSs, these changes were less pronounced. An RNAi response efficiently reduced virus replication in U4.4 cells transfected with virus specific dsRNA, but not in C6/36 or C7/10 cells. Lastly, Ae. aegypti mosquitoes were exposed to blood-meal containing either wild-type or NSs deletion virus, and at various times post-feeding, infection and disseminated infection rates were measured. Compared to wild-type virus, infection rates by the mutant virus were lower and more variable. If the NSs deletion virus was able to establish infection, it was detected in salivary glands at 6 days post-infection, 3 days later than wild-type virus.Conclusions/SignificanceBunyamwera virus NSs is required for efficient replication in certain mosquito cell lines and in Ae. aegypti mosquitoes.

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“…Comprehensive molecular genetic studies have involved viruses in only four serogroups, namely, Bunyamwera, group C, California, and Simbu (5,12,26,27), but recently S-segment nucleotide sequences have been obtained for one or two representatives of the Bakau, Bwamba, Nyando, and Turlock serogroups (20,35). In all cases the S genome segment encodes two proteins, N and, in an overlapping open reading frame (ORF), a small nonstructural protein termed NSs.…”

mentioning

confidence: 99%