Molecular characterization of allelic status of the Rpf1 and Rca2 genes in six cultivars of strawberries

2021

BIO Web Conf.

The results of the analysis of allelic polymorphism of the Rpf1 red stele root rot resistance gene in strawberry hybrid forms using diagnostic DNA markers were shown. The recessive homozygous genotype (rpf1rpf1) was identified in strawberry seedlings 932-29 (F. virginiana subsp. platypetala (Rydb.) Staudt × Feyerverk), 933-4 (F. virginiana subsp. platypetala (Rydb.) Staudt × Rubinovyy kulon), 62-7, 62-23 (Bylinnaya × Feyerverk), 65-2, 65-15 (Olimpiyskaya nadezhda × Bylinnaya) and 35-16 (922-67 × Maryshka). The heterozygous state of the Rpf1 gene was identified in strawberry forms 69-29 (Feyerverk × Bylinnaya), 62-41 (Bylinnaya × Feyerverk), 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 65-17, 65-24 (Olimpiyskaya nadezhda × Bylinnaya), which allows them to be used in breeding for resistance to P. fragariae var. fragariae as valuable initial forms.

Section: Resultsmentioning

confidence: 99%

“…The OPO-16C marker (438 bp amplicon) corresponds to the non-functional rpf1 allele. The combination of these markers makes it possible to identify the allelic state of the Rpf1 gene in the studied strawberry genotypes [13].…”

Section: Methodsmentioning

confidence: 99%

Marker controlled screening of resistant to red stele root rot (Rpf1 gene) strawberry selected forms

2021

BIO Web Conf.

“…In Europe and the Eurasian Economic Union, C. acutatum is a quarantine pathogen [11,12].According to the studies of European researchers, the strawberry resistance to some isolates of C. acutatum is controlled by the dominant Rca2 gene. Identification of genetic determinants of this trait allows for targeted selection of promising strawberry forms using diagnostic molecular markers [13][14][15].The purpose of the study was assess the allelic diversity of the Rca2 anthracnose resistance gene in strawberry varieties to identify promising genotypes for involvement in the strawberry breeding to create resistant to fungal pathogens forms.The studies were carried out in 2020-2021. Biological material was represented by promising strawberry varieties from genetic collection of the FSSI "I.V.…”

mentioning

confidence: 99%

“…According to the studies of European researchers, the strawberry resistance to some isolates of C. acutatum is controlled by the dominant Rca2 gene. Identification of genetic determinants of this trait allows for targeted selection of promising strawberry forms using diagnostic molecular markers [13][14][15].…”

mentioning

confidence: 99%

Assessment of strawberry varieties by anthracnose resistance gene

2021

BIO Web Conf.

The results of marker-assisted identification of the Rca2 anthracnose resistance gene in promising strawberry varieties were shown. The DNA marker STS-Rca2_240, linked to the Rca2 anthracnose resistance allele was identified in varieties Albion and Aprica (F. × ananassa), and variety Dar (F. × anashata). Strawberry varieties Alpha, Bereginya, Kokinskaya Zarya, Pamyati Zubova, Rubinovy kaskad, Slavutich, Yuniol, Big King, Brilla, Joly, Rumba and Vivara are characterized by the absence of the marker STS-Rca2_240. In strawberry varieties with the identified marker STS-Rca2_240 (Dar, Albion and Aprica), the Rca2 gene can be in a dominant homozygous or heterozygous state, which makes it possible to recommend these genotypes for use in breeding for anthracnose resistance.

“…(у 58,3 % форм). Возможность амплификации у отдельных генотипов земляники дополнительных продуктов маркера OPO-16C подтверждается другими исследователями: Sturzeanu et al [20] дополнительные фрагменты размером около 600 и более 1500 п.н. выявили у сортов Benton и Mira, в исследовании Gelvonauskienė et al [21] мономорфный фрагмент размером около 600 п.н.…”

unclassified

Analysis of polymorphism of strawberry genotypes (Fragaria L.) according to the strawberry red root spot resistance gene RPF1 for identification of strawberry forms promising for breeding and horticulture

Vescì Nacyânalʹnaj akadèmìì navuk Belarusì. Seryâ agrarnyh navu

2020

Red root spot (Phytophthora fragariae var. fragariae Hickman) is one of the most important strawberry diseases in the temperate climate zone. Identification of forms, carrying resistance genes, is an important stage in breeding programs aimed at obtaining red root spot resistant strawberry varieties. Diagnostic DNA markers of target genes will increase reliability of identification and efficiency of strawberry breeding for the creation of resistant genotypes. The purpose of this study is analysis of polymorphism of wild species of Fragaria L. genus and strawberry varieties (F. × ananassa Duch.) according to the strawberry red root spot resistance gene Rpf1 using molecular markers. The research sunjects were the wild species F. orientalis Los., F. moschata Duch., F. ovalis Rydb., F. virginiana Duch. ssp. platypetala and strawberry varieties (F. × ananassa Duch.) of different ecological and geographic origin. Total genomic DNA of strawberry was extracted from the fresh leaves using the Puchooa method. To assess the allelic state of Rpf1 gene, SCAR-R1A marker (linked to the Rpf1 dominant allele) and OPO-16C marker (linked to the rpf1 recessive allele) have been used. SCAR-R1A marker was identified in wild species F. virginiana Duch. ssp. platypetala (vegetation region: British Columbia, Canada), pineapple strawberry varieties Bylinnaya and promising selected forms 62-41 (Bylinnaya × Feyyerverk), 65-17 and 65-24 (Olimpiyskaya nadezhda × Bylinnaya). These genotypes are characterized by heterozygous Rpf1rpf1 genotype according to Rpf1 gene (both markers are present in genotype) and can be used as red root spot resistance source in marker-assisted selection. In the remaining studied genotypes of strawberry, SCAR-R1A marker was not detected, which presumably indicated their homozygous recessive genotype rpf1rpf1 according to Rpf1 gene. The research results can be useful for breeders of strawberry and researchers of plant biodiversity of p. Fragaria.