Molecular characterization of an Indian isolate of Banana bunchy top virus based on six genomic DNA components

Abstract

Banana bunchy top virus (BBTV) is a single-stranded circular DNA virus of the genus Babuvirus, belonging to family Nanoviridae. The six genomic DNA components of Indian (Lucknow) isolate of BBTV were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of banana bunchy top disease (BBTD). The resulting ~1.1 Kb amplicons were cloned and sequenced. Analysis of sequence data revealed the presence of six full-length components of BBTV: DNA-R (1111 bp), DNA-U3 (1060 bp), DNA-S (1075 bp), DNA-M (1048 bp), DNA-C (1018 bp), and DNA-N (1096 bp). Comparisons of sequence data of the six DNA components of the BBTV Lucknow isolate revealed highest identities with sequences of other BBTV isolates from the South Pacific group: [DNA-R (98%), DNA-U3 (93%), DNA-S (100%), DNA-M (98%), DNA-C (97%), and DNA-N (99%)]. A phylogenetic analysis revealed a close relationship of the Lucknow isolate with BBTV isolates of South Pacific group rather than those of the Asian group. Based on these analyses the virus has been classified as BBTV Lucknow, a new member of South Pacific group.

Molecular Characterization of Geographically Different Banana bunchy top virus Isolates in India

“…All the six components of HB-TN isolate clustered with isolates of the South Pacific group and the isolates of Asian group clustered as separate clade. Our results corroborated with the results of Vishoni et al [19] who have reported that the BBTV isolate occurring in Lucknow, North India was belong to the South Pacific group. The species demarcation criteria for nanoviruses was based on amino acid identities of the CP gene and the members of distinct species should be \85% similarity (Vetten et al 18).…”

“…In India, BBTV has been prevalent since 1943 and so it is possible that genetic variability such as that observed among isolates of the Asian group exists. Recently, the complete genomes of BBTV isolates occurring in Pakistan [1] and Lucknow, North India [19] were reported and found to belong to the South Pacific group.…”

Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

“…In the present study, all six components of BBTV-K isolate were evaluated. Sequence analysis of BBTV-K isolate revealed that it is as similar as other reported genomes of BBTV [41]. Burns et al [10] demonstrated that the genome of BBTV comprises at least five ssDNA components based on the restriction digests of putative full-length clones synthesized from purified virions.…”

“…Based on phylogenetic analysis of DNA-R, Karan et al [26] divided BBTV isolates into two groups such as a PIO group and South-East Asian (SEA) group. BBTV isolates from India were found to be belonging to the PIO group [6,37,41]. Similarly, Amin et al [3] characterized the Pakistan (Sindh) isolate of BBTV by analyzing of sequences of DNA components and indicated that BBTV present in Pakistan belongs to the PIO group.…”

Advances in Small Isometric Multicomponent ssDNA Viruses Infecting Plants

“…1). BBTV is widely distributed in banana growing countries in the Asia, Pacific region (Australia, China, Guam, India, Pakistan, Sri Lanka, Hawaii, Indonesia, Japan, the Philippines, Taiwan, Tonga, Samoa and Vietnam) and in a few countries in Africa (Egypt, Gabon, Angola and Burundi) [2,16,50,89,99]. There are many other banana growing regions, South and Central America, the Caribbean, Papua New Guinea, Thailand and in many countries in African and Asia, where BBTV has not been confirmed [43,89].…”