Molecular characterization of an uncommon multigene Reassortant G1P[4] rotavirus identified in China

Zeng, Yuanjun; Zhao, Biyan; Li, Tingdong; Zhang, Shiyin; Wang, Yingbin; Xu, Hongmei; Ge, Shengxiang; Xia, Ningshao

doi:10.1016/j.meegid.2020.104413

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…In China, a multireassortant G1P [4] strain was identi ed in 2013. Most likely, it originated from locally co-circulated G1P[8] and G2P [4] rotaviruses [52]. Multiple G1P[8], G2P [4], G2P[8], G4P [4], G9P [4], and G12P[8] reassortants were found in Rwanda in 2011-2016 [53,54].…”

Section: Discussionmentioning

confidence: 99%

Detection and full-genotype determination of rare and reassortant rotavirus A strains in Nizhny Novgorod in the European part of Russia

Sashina,

Velikzhanina,

Morozova

et al. 2023

Arch Virol

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Reassortant DS-1-like Rotavirus A strains have been shown to widely circulate in many countries around the world. In Russia, the real contribution of such strains remains unclear due to preferred use of the traditional binary classi cation only. In this work, we obtained data on partial sequencing of all 11 genome segments and presented the full-genotype constellations of rare and reassortant rotaviruses circulating in Nizhny Novgorod in 2016-2019. DS-1-like G3P[8] and G8P[8] strains were found, and it emphasized the global trend. Most likely, they were introduced into the territory of Russia from other countries, but subsequently underwent further evolutionary changes locally. G3P[8], G9P[8], and G12P[8] Wa-like strains withunusual for the territory of Russia subgenotypic lineages were also identi ed.Reassortant G2P[8], G4P[4], and G9P[4] strains with one Wa-like gene (VP4 or VP7) on a DS-1-like backbone were found and apparently had alocal origin. Feline-like G3P[9] and G6P[9] strains were phylogenetically close to BA222 isolated from Italian cat but carried some traces of reassortment with human strains from Russia and other countries. Thus, full-genotype investigation of Nizhny Novgorod Rotavirus A strains clari ed some questions related to their origin and evolution.

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Section: Discussionmentioning

confidence: 99%

Detection and full-genotype determination of rare and reassortant rotavirus A strains in Nizhny Novgorod in the European part of Russia

Sashina,

Velikzhanina,

Morozova

et al. 2023

Arch Virol

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Identification of a cooperative effect between amino acids 169 and 174 in the rotavirus NSP4 double-layered particle-binding domain

Herbert,

van Dijk

2024

Journal of General Virology

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Segmented RNA viruses are capable of exchanging genome segments via reassortment as a means of immune evasion and to maintain viral fitness. Reassortments of single-genome segments are common among group A rotaviruses. Multiple instances of co-reassortment of two genome segments, GS6(VP6) and GS10(NSP4), have been documented in surveillance. Specifically, a division between NSP4 genotypes has been observed in the NSP4 double-layered particle (DLP)-binding domain. A previously hypothesized mechanism for this co-reassortment has been suggested to be the interaction between VP6 and NSP4 during DLP transport from viroplasms for particle maturation. In this study, we used sequence analysis, RNA secondary structure prediction, molecular dynamics and reverse genetics to form a hypothesis regarding the role of the NSP4 DLP-binding domain. Sequence analysis showed that the polarity of NSP4 DLP-binding domain amino acids 169 and 174 is clearly divided between E1 and E2 NSP4 genotypes. Viruses with E1 NSP4s had 169A/I or 169S/T with 174S. E2 NSP4s had 169R/K and 174A. RNA secondary structure prediction showed that mutation in both 545 (aa169) and 561 (aa174) causes global structure remodelling. Molecular dynamics showed that the NSP4/VP6 interaction stability is increased by mutating both aa positions 169 and 174. Using reverse genetics, we showed that an R169I mutation alone does not prevent rescue. Conversely, 174A to 174S prevented rescue, and rescue could be returned by combining 174S with 169I. When compared to rSA11 NSP4-wt, both rSA11 NSP4-R169I and rSA11 NSP4-R169I/A174S had a negligible but significant reduction in titre at specific time points. This study suggests that amino acid 174 of NSP4 may be essential in maintaining the VP6/NSP4 interaction required for DLP transport. Our results suggest that maintenance of specific polarities of amino acids at positions 169 and 174 may be required for the fitness of rotavirus field strains.

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Detection and full-genotype characterization of rare and reassortant Rotavirus A strains in Nizhny Novgorod, European part of Russia

Сашина

Velikzhanina

Морозова

et al. 2023

Preprint

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Reassortant DS-1-like Rotavirus A strains have been shown to widely circulate in many countries around the world. In Russia, the real contribution of such strains remains unclear due to preferred use of the traditional binary classification only. In this work, we obtained data on partial sequencing of all 11 genome segments and presented the full-genotype constellations of rare and reassortant rotaviruses circulating in Nizhny Novgorod in 2016-2019. DS-1-like G3P[8] and G8P[8] strains were found, and it emphasized the global trend. Most likely, they were introduced into the territory of Russia from other countries, but subsequently underwent further evolutionary changes locally. G3P[8], G9P[8], and G12P[8] Wa-like strains withunusual for the territory of Russia subgenotypic lineages were also identified. Reassortant G2P[8], G4P[4], and G9P[4] strains with one Wa-like gene (VP4 or VP7) on a DS-1-like backbone were found and apparently had alocal origin. Feline-like G3P[9] and G6P[9] strains were phylogenetically close to BA222 isolated from Italian cat but carried some traces of reassortment with human strains from Russia and other countries. Thus, full-genotype investigation of Nizhny Novgorod Rotavirus A strains clarified some questions related to their origin and evolution.

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Molecular characterization of an uncommon multigene Reassortant G1P[4] rotavirus identified in China

Cited by 3 publications

References 12 publications

Detection and full-genotype determination of rare and reassortant rotavirus A strains in Nizhny Novgorod in the European part of Russia

Detection and full-genotype determination of rare and reassortant rotavirus A strains in Nizhny Novgorod in the European part of Russia

Identification of a cooperative effect between amino acids 169 and 174 in the rotavirus NSP4 double-layered particle-binding domain

Detection and full-genotype characterization of rare and reassortant Rotavirus A strains in Nizhny Novgorod, European part of Russia

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