Molecular Characterization of Ancylostoma braziliense Larvae in a Patient with Hookworm-Related Cutaneous Larva Migrans

Abstract

Abstract. We report a case of hookworm-related cutaneous larva migrans diagnosed microscopically. Viable hookworm larvae were found by microscopic examination of a skin scraping from follicular lesions. Amplification and sequencing of the internal transcribed spacer 2 allowed the specific identification of the larvae as Ancylostoma braziliense.

Dermoscopy and near‐infrared fluorescence imaging of cutaneous larva migrans

“…The two small areas of bright fluorescence seen in our patient under NIR fluorescence imaging are similar to those seen in pigmented skin lesions. In addition, the size of the bright dots is similar to the width of the larva reported in the literature (4). We hypothesize that the NIR fluorescence originated from melanin ingested by the larva while migrating within the epidermis.…”

“…Two small oval bright spots oriented in a similar fashion as the dermoscopic structures in relation to the white label. The size of the bright dots is similar to the width of the larva reported in the literature (4). The white label is 7 mm in diameter and appears black with near-infrared fluorescence imaging.…”

Aetiologies of creeping eruption: 78 cases

“…Hookworm folliculitis was defined by pruritic follicular papules and pustules associated with serpiginous burrows. The diagnosis was confirmed by microscopic examination of a skin scraping from follicular lesions as previously described …”

“…The diagnosis was confirmed by microscopic examination of a skin scraping from follicular lesions as previously described. 7 A diagnosis of gnathostomiasis was raised in cases with the following information: epidemiological characteristics involving a history of eating raw freshwater fish or shellfish during travel in an endemic country; clinical features of creeping eruption, oedema, migratory swellings and panniculitis; and blood eosinophilia. The diagnosis was confirmed by a serological test performed in Bangkok.…”

Case of Th2 Cytokines in a Japanese Girl Who Acquired Hookworm-Related Cutaneous Larva

“…We discussed regarding the type of parasite that may have caused HrCLM and transition of Th2 cytokines in the current case. DNA was extracted from paraffin-embedded tissue at resection, and polymerase chain reaction (PCR) was performed using specific primers for A. ceylanium [4][5][6], A. caninum [4][5][6], A. Brasiliense [6,7], A. duodenale [4], U. stenocephala [6], and N. americanus [4]; however, no amplification was detected. For further investigation, the eosinophil count, IgE level, and serum immune-related Th2 cytokine levels (IL-3, IL-4, IL-5, IL-13, IL-18, and IL-33) were measured at the first visit, after 2 days postoperatively, at the time of recurrence (after 9 days), after the first dose of ivermectin (after 16 days), and after the second dose of ivermectin (after 42 days).…”