Molecular characterization of begomovirus–betasatellite–alphasatellite complex associated with okra enation leaf curl disease in Northern Sri Lanka

Abstract

Okra enation leaf curl is a newly emerging disease in commercial okra cultivation fields in Northern Sri Lanka. The present study aimed to identify and characterize the causative begomovirus and associated satellites. Okra plants showing the enation leaf curl disease symptoms were collected from Vavuniya and Jaffna districts of Northern Province. The PCR diagnostic and genome sequencing revealed that the symptomatic okra plants are associated with begomovirus, betasatellite, and alphasatellite complex. The begomovirus isolates shared 98.2-99.7% nucleotide identity with Okra enation leaf curl virus. The betasatellites showed 96-98.8% nucleotide identity with Bhendi yellow vein mosaic betasatellite which is usually associated with Bhendi yellow vein mosaic disease. Two distinct alphasatellite species, Okra leaf curl alphasatellite and Bhendi yellow vein mosaic alphasatellite, were identified in leaf samples with enation leaf curl disease. The disease was transmitted by whiteflies from diseased plants to healthy plants. Hybrid varieties were more susceptible to the disease compared to cultivated varieties.

Resistant Genotypes and Botanicals Are the Potential Hindrances Against Vector Transmitted Okra Yellow Vein Mosaic Virus

“…OYVMV impairs the photosynthetic activity and frequency by disturbing light harvesting capacity of the chlorophyll that ultimately results in reduced energy for driving the metabolic activities. The reduced metabolism leads to disturbance in physiology of plants and lower growth, development and yield of okra plant (Emmanuel et al, 2020). The yield decrease and quality deterioration of okra fruits depend upon the virulence of the viral pathogen, susceptibility of the cultivar and duration of feasible weather conditions that prevail during OYVMV and okra interaction.…”