Molecular Characterization of Begomoviruses DNA-A and Associated Beta Satellites with New Host Ocimum sanctum in India

Genome of a known but distinct begomovirus associated with a novel satellite molecule infecting a new host bitter gourd (Momordica charantia)

“…All the members of the New World begomoviruses are mostly bipartite, and Old World begomoviruses have either monopartite or bipartite genomes that interact with single-stranded DNA (ssDNA) satellites namely alpha-and beta-satellite. Both the satellite molecules depend upon their helper virus for replication and/or movement (Rojas et al 2001;Marwal et al 2018). Mixed infections with two or more virus species have been observed in several hosts, and symptoms are more rigorous than those observed in single viral infections in some occasions; however, mixed infections with begomoviruses have not been studied comprehensively in India (Rentería-Canett et al 2011a, b).…”

“…To determine the existence of recombination events in the reported viral sequences, RDP analysis was conducted using RDP4.2 program (Nehra et al 2018). The analysis was based on alignments with full length sequences of selected begomoviruses available in the NCBI database.…”

Complete nucleotide sequence of a new geminivirus isolated from Vitis vinifera in India: a symptomless host of Grapevine red blotch virus

“…The entire 45 samples were subjected to Rolling Circle Amplification using a TempliPhi DNA amplification kit (GE Healthcare, Little Chalfont, Bukinghamshire, UK), as per the manufacturer's instructions [18]; remarkably, 1 of the samples was amplified. To identify the possible geminivirus for better assessment and to authenticate the results, entire grapevine plantation from the field number 4 was screened.…”