Molecular characterization of biosurfactant producing marine bacterium isolated from hydrocarbon-contaminated soil using 16S rRNA gene sequencing

Balakrishnan, Sethuramalingam; Arunagirinathan, Narasingam; Rameshkumar, Marimuthu Ragavan; Purushothaman, Indu; Vijaykanth, Nallusamy; Almaary, Khalid S.; Almutairi, Saeedah Musaed; Chen, Shen‐Ming

doi:10.1016/j.jksus.2022.101871

Cited by 13 publications

(2 citation statements)

References 17 publications

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“…al. [39], the amplicon size of the 16S rRNA gene is in the range of 1500 bp which consists of a conserved region. The amplification of the 16S rRNA gene was declared successful if the amplicon visualized by gel electrophoresis showed a size of 1500 bp.…”

Section: Molecular 16s Rrna Identificationmentioning

confidence: 99%

Antibacterial Activity and Molecular Identification of Soft Coral Sinularia sp. Symbiont Bacteria from Karimunjawa Island against Skin Pathogens Propionibacterium acnes and Staphylococcus epidermidis

Wulandari,

Amatullah,

Lunggani

et al. 2024

BIO Web Conf.

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Soft corals can produce bioactive compounds that act as antibacterials. Sinularia sp. is a soft coral that can produce antibacterial compounds. Symbion bacteria are bacteria that can produce bioactive compounds that tend to be the same as their host. The use of symbiont bacteria can be an alternative to exploit the potential of Sinularia sp. without harming marine life. This study aims to determine the antibacterial potential of the symbiont bacteria obtained from the soft coral Sinularia sp. in inhibiting the growth of Propionibacterium acnes and Staphylococcus epidermidis. The antibacterial activity test was carried out by the agar diffusion method using disc paper. Antibacterial activity was indicated by the formation of a clear zone around the disc paper. The results showed that LA7 isolate had the best antibacterial activity with an average clear zone size of 14.6 mm against Staphylococcus epidermidis and 16.6 mm against Propionibacterium acnes. LA7 isolates identified species based on the 16S rRNA gene with primers 27F and 1492R. Based on molecular species support, it was found that LA7 isolate is Bacillus aerius with a homology level of 98.17%.

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Section: Molecular 16s Rrna Identificationmentioning

confidence: 99%

Antibacterial Activity and Molecular Identification of Soft Coral Sinularia sp. Symbiont Bacteria from Karimunjawa Island against Skin Pathogens Propionibacterium acnes and Staphylococcus epidermidis

Wulandari,

Amatullah,

Lunggani

et al. 2024

BIO Web Conf.

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“…Pure cultures were further tested for their potential to form biosurfactants and later identified by molecular methods for the identification of all strains. Balakrishnan and workers 47 reported seven unique colonies based on their morphology from hydrocarbon-contaminated soil. These bacteria could utilize hydrocarbon as a nutrient source and grow and form colonies with different morphological features ranging from irregular, opaque, pigmented light green colonies to nonpigmented forms.…”

Section: Morphology Of Isolated Bacterialmentioning

confidence: 99%

Isolation, Screening, Characterization, and Optimization of Biosurfactant-Producing Bacteria for Removal of Micropollutants from Textile Effluents

Kumari,

Debnath,

Joshi

et al. 2024

Ind. Eng. Chem. Res.

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Biosurfactants are ecofriendly substitutes for chemical surfactants. In this study, biosurfactant-producing bacterial strains were isolated from soil and water samples from textile industry sites. Extracted biosurfactants could reduce the surface tension values from 72 to 39.3 mN/m demonstrating that the biosurfactants achieved the critical micelle concentration. Stable emulsifications with diesel and vegetable oil showed an emulsification index ranging from 61.76% to 76.47%. The presence of both rhamnolipid and surfactin types of biosurfactants was confirmed from our isolates using FTIR, LC-MS/MS, and GC-MS/MS analyses. These biosurfactant-producing bacteria act on dyes and heavy metals present in textile wastewater and reduce their concentrations by either degradation or biosorption. After the reaction with biosurfactant-producing bacterial isolates, FTIR spectra confirmed the breakdown of some compounds and their conversion into intermediates. There was also a reduction in the concentration of metals in textile effluents, as shown by EDAX analysis. These novel bacteria can be scaled up for their use in water reclamation and recycled for regular industrial use.

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Potential biological active bacteriocin production by Bifidobacterium via eco‐friendly, low‐cost solid state fermentation principle

Priyanka,

Namasivayam,

Sudha

et al. 2024

Environmental Quality Mgmt

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The present study is undertaken to evaluate the potential biological activities of bacteriocin produced by the Lactic acid bacteria Bifidobacterium sp. adopting solid state fermentation. Bifidobacterium was isolated from cooked fermented rice water. Isolated bacterial strain was identified by morphological, biochemical, and molecular characteristics. Sterilized cooked rice was inoculated with bacterial inoculum followed by extraction of bacteriocin under aseptic condition. Extracted bacteriocin was screened for antibacterial, antioxidant, and anti‐inflammatory activities under invitro condition. Cooked fermentation rice medium used for solid state fermentation (SSF) supported growth and bacteriocin production by Bifidobacterium longum. Potential antibacterial activity of the extracted bacteriocin was confirmed by growth inhibition of Bacillus subtilis, and Escherichia coli strain. Notable free radical scavenging activity at 120 μg/mL concentration of bacteriocin clearly indicated antioxidant activity. Anti‐inflammatory activity of the bacteriocin was determined which showed maximum inhibition at 120 μg/mL concentration. All these finding suggest the possible utilization of bacteriocin extraction from Bifidobacterium as an effective, safe bioactive agent.

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Molecular characterization of biosurfactant producing marine bacterium isolated from hydrocarbon-contaminated soil using 16S rRNA gene sequencing

Cited by 13 publications

References 17 publications

Antibacterial Activity and Molecular Identification of Soft Coral Sinularia sp. Symbiont Bacteria from Karimunjawa Island against Skin Pathogens Propionibacterium acnes and Staphylococcus epidermidis

Antibacterial Activity and Molecular Identification of Soft Coral Sinularia sp. Symbiont Bacteria from Karimunjawa Island against Skin Pathogens Propionibacterium acnes and Staphylococcus epidermidis

Isolation, Screening, Characterization, and Optimization of Biosurfactant-Producing Bacteria for Removal of Micropollutants from Textile Effluents

Potential biological active bacteriocin production by Bifidobacterium via eco‐friendly, low‐cost solid state fermentation principle

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