Molecular Characterization of Border Disease Virus, a Pestivirus from Sheep

Becher, Paul; Shannon, Anthony; Tautz, Norbert; Thiel, Heinz-Jürgen

doi:10.1006/viro.1994.1065

Cited by 128 publications

(106 citation statements)

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“…CSFV specific primers were designed considering sequences of the 5'NTR of the genome of 55 different pestivirus strains, available in GenBank or in the database of CSF European reference laboratory of Hanover (Germany), that includes 37 CSFV representing subgroups described by Lowings [20]: 1.1, 1.2, 2.1, 2.2, 2.3 and disparate virus outgrouped [12,13,15,25,27,[31][32][33], fourteen BVDV belonging to types I and II [5,8,32] and four BDV isolates [4,29]. Sequences were aligned, using Clustal W software, and five oligonucleotides were primarily selected from highly conserved sequences among the different CSFV strains but showing high divergence with the other pestiviruses.…”

Section: Primers and Restriction Endonuclease Selectionmentioning

confidence: 99%

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Agüero

Fernández²,

Romero

et al. 2004

Vet. Res.

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-The development and standardisation of a novel, highly sensitive and specific one-step hot start multiplex RT-PCR assay is presented for the simultaneous and differential diagnosis of African swine fever (ASF) and Classical swine fever (CSF). The method uses two primer sets, each one specific for the corresponding virus, amplifying DNA fragments different in length, allowing a gel-based differential detection of the PCR products. Universal detection of ASF and CSF virus strains was achieved through selection of primers in conserved viral genome regions. The detection range was confirmed by analysis of a large collection of isolates of the two viruses. The high specificity of the assay was proven by testing related viruses, uninfected cell line cultures and healthy pig tissues. Additional confirmatory tests of the ASF and CSF virus amplicon specificity, based on restriction endonuclease analysis with BsmA I or Ban II, respectively, are also described. The analysis of whole blood and serum samples from experimentally infected animals proved the usefulness of the method for an early diagnosis of both diseases, even before the appearance of the first clinical signs. A study of 150 positive field samples from several ASF and CSF outbreaks showed the suitability of this method for a rapid (less than five hours), sensitive and specific differential diagnosis in clinical samples. In addition, a highly sensitive and specific uniplex RT-PCR for CSFV was also developed and standardised as a powerful tool for fast and early diagnosis of the disease.

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Section: Primers and Restriction Endonuclease Selectionmentioning

confidence: 99%

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Agüero

Fernández²,

Romero

et al. 2004

Vet. Res.

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“…The genome is a positive singlestranded RNA molecule with a length of 12.3 kb which encodes a polyprotein of about 4000 amino acids [2]. Many advances have been realized in the molecular structure of BDV [20,32], and genetic diversity of pestiviruses and the relationship between isolates have been extensively studied [3,10,18].…”

Section: Introductionmentioning

confidence: 99%

Experimental model of Border Disease Virus infection in lambs: comparative pathogenicity of pestiviruses isolated in France and Tunisia

Thabti

Fronzaroli²,

Dlissi

et al. 2002

Vet. Res.

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-Pestiviruses have been isolated from live sheep pox Tunisian vaccines. Vaccination with these vaccines caused outbreaks of Border Disease in Tunisia. In order to study more precisely the pathogenicity of these isolates, three groups of eight four month old lambs from a pestivirus-free flock were infected by the intratracheal route with a French strain (AV) and two Tunisian isolates (SN3G and Lot21). Clinical, hematological, immunological and virological parameters were evaluated. The three groups developed mild fever and leucopaenia by day 3 to 6 post infection (pi). The differences in the weight curves were not significant. Viruses were isolated from the peripheral blood buffy coat cells by day 4 to 9 pi. Antibodies were present on day 16 pi following infection by the French strain and on day 21 pi with the Tunisian isolates. The results demonstrated that SN3G and Lot21 are almost similar to the French strain used as the reference strain. In field conditions, they could induce economical losses in naive flocks, alone or in association with other pathogens. (33) 492943701; e-mail: p.russo@sophia.afssa.fr 8 agneaux de 4 mois issus d'un troupeau indemne de pestivirus ont été infectés par voie intratrachéale avec une souche française de référence (AV) et 2 isolats tunisiens (SN3G et Lot21). Les données cliniques, hématologiques, immunologiques et virologiques ont été suivies. Les 3 groupes ont développé une légère hyperthermie et une leucopénie entre le 3 e et le 6 e jour post inoculation (pi). Les courbes de poids ne montraient pas de différence significative. Des pestivirus ont été isolés à partir des cellules blanches du sang périphérique entre le 4 e et le 9 e jour pi. Des anticorps ont été mis en évi-dence 16 jours pi avec la souche française et 21 jours pi avec les isolats tunisiens. L'ensemble des ré-sultats a montré que les 2 isolats tunisiens ont un pouvoir pathogène presque similaire à celui de la souche française; ils pourraient provoquer, dans les conditions du terrain, des pertes économiques, seuls ou en association avec d'autres agents pathogènes.Pestivirus / ovin / vaccins anti-clavelée / pouvoir pathogène

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“…BVDV strain 5250 isolated from pigs was efficiently inhibited by CSFV E rns , whereas several BVDV strains isolated from cattle were not, suggesting that the conformation of E rns plays a role in host tropism. (Becher et al, 1994 ;Collett et al, 1988 b ;Ru$ menapf et al, 1993 ;Stark et al, 1990). Although all three glycoproteins are associated with the virus envelope , up to now only antibodies against the two largest glycoproteins have been detected in animals infected with pestiviruses (Kwang et al, 1992 ;Terpstra & Wensvoort, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…The genome of pestiviruses varies in length from 12n5 to 16n5 kb (Becher et al, 1994 ;Collett et al, 1988 a ;Meyers et al, 1989 ;Moormann & Hulst, 1988 ;Qi et al, 1992 ;Renard et al, 1985) and contains a single large open reading frame which after translation is converted into mature proteins by virus and host cell proteases (Ru$ menapf et al, 1993). The core protein, C, and three glycoproteins, E rns , E1 and E2, which are the structural proteins, are encoded in the 5' region of the genome Author for correspondence : R. J. M. Moormann.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of pestivirus infection in cell culture by envelope proteins E(rns) and E2 of classical swine fever virus: E(rns) and E2 interact with different receptors.

Hulst¹,

Moormann²

1997

Journal of General Virology

125

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Pure preparations of envelope glycoproteins E rns and E2 of classical swine fever virus (CSFV) synthesized in insect cells were used to study infection of porcine and bovine cells with the pestiviruses CSFV and bovine viral diarrhoea virus (BVDV). Almost 100 % inhibition of infection of porcine kidney cells with CSFV was produced by 100 µg/ml E rns . After removal of the virus no E rns was needed in the overlay medium (growth medium) to maintain this level of inhibition. In contrast, 100 % inhibition of infection of porcine kidney cells with CSFV by 10 µg/ml E2 was only achieved when E2 was added to the overlay medium. When E2 was omitted, a maximum of 50 % inhibition was achieved. This indicated that after the virus and E2 were removed from the cells, infection still occurred, by virus

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Molecular Characterization of Border Disease Virus, a Pestivirus from Sheep

Cited by 128 publications

References 0 publications

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Experimental model of Border Disease Virus infection in lambs: comparative pathogenicity of pestiviruses isolated in France and Tunisia

Inhibition of pestivirus infection in cell culture by envelope proteins E(rns) and E2 of classical swine fever virus: E(rns) and E2 interact with different receptors.

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