Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers

Abstract

Buffalograss [ Buchloe dactyloides (Nutt.) Englem] germplasm has a broad resource of genetic diversity that can be used for turfgrass, forage and conservation. Buffalograss is the only native grass that is presently used as a turfgrass in the Great Plains region of North America. Its low growth habit, drought tolerance and reduced requirement for fertilizer and pesticides contribute to interest in its use. The objectives of this study were to use sequence-related amplified polymorphism (SRAP) markers in the evaluation of genetic diversity and phenetic relationships in a diverse collection of 53 buffalograss germplasms, and to identify buffalograss ploidy levels using flow cytometry. Based on their DNA contents, buffalograss genotypes were grouped into four sets, corresponding to their ploidy levels. Thirty-four SRAP primer combinations were used. This is the first report of the detection of differentiating diploid, tetraploid, pentaploid and hexaploid buffalograss genotypes, representing diverse locations of origin, using SRAP markers. Cluster analysis by the unweighted pair-group method with arithmetic averages based on genetic similarity matrices indicated that there were eight clusters. The coefficients of genetic distance among the genotypes ranged from 0.33 up to 0.99 and averaged D=0.66. The genetic diversity estimate, He, averaged 0.35. These results demonstrated that genotypes with potential traits for turfgrass improvement could readily be distinguished, based on SRAP. The use of PCR-based technologies such as SRAP is an effective tool for estimating genetic diversity, identifying unique genotypes as new sources of alleles for enhancing turf characteristics, and for analyzing the evolutionary and historical development of cultivars at the genomic level in a buffalograss breeding program.

Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

“…In addition, sequence-related amplified polymorphism (SRAP) screening was carried out on the parents (Budak et al 2004) for a total of 238 primer combinations. The PCR amplification conditions were as described by Li and Quiros (2001).…”

Mapping QTLs for mineral accumulation and shoot dry biomass under different Zn nutritional conditions in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

“…Seven Sequence Related Amplified Polymorphism (SRAP) primer combinations (me1/em1, me1/em6, me4/em1, me4/em6, BMe6/BEm12, BMe10/CoEm9, CoMe10/K6), distinguishing the parents, were used to test the DH lines following Li and Quiros (2001). The markers originated from B. oleracea , B. napus (Riaz et al 2001), buffalo grass (Budak et al 2004) and cotton (Lin et al 2003). SRAP markers were scored on the basis of the presence or absence of the band and were named using the primer combination followed by a number in descending molecularweight order.…”

Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]

“…SRAP markers are more powerful for revealing genetic diversity among closely related cultivars than SSR, ISSR, or RAPD markers (Budak et al 2004) and can be used for linkage map construction (Yeboah et al 2007), genomic and cDNA fingerprinting, gene tagging (Li and Quiros 2001), genetic diversity analysis (Li et al 2009), map-based cloning (Zhang et al 2010), hybrid identification (Mishra et al 2011) and sex determination (Zhou et al 2011). In our study, SRAP markers were used for the first time to analyse genetic diversity in important cultivars and advanced breeding lines of mungbean varying in micronutrient (Fe and Zn) content.…”