Molecular characterization of Carbapenem resistant Escherichia coli recovered from a tertiary hospital in Lebanon

Dagher, Christel; Salloum, Tamara; Alousi, Sahar; Arabaghian, Harout; Araj, George F.; Tokajian, Sima

doi:10.1371/journal.pone.0203323

Cited by 46 publications

(34 citation statements)

References 56 publications

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“…In the carbapenemase-producers, the clinical isolates belonged to groups C (50%) and D (30%) while the environmental isolates were mainly groups B1, D, and A (Fig 6B ). A previous study showed that carbapenemase positive isolates distributed in groups A, D, B2, and B1 in descending order [44]. Only one isolate was unassigned to any phylogenetic group.…”

Section: Plos Onementioning

confidence: 90%

Genetic characterization of extended-spectrum β-Lactamase- and carbapenemase-producing Escherichia coli isolated from Egyptian hospitals and environments

et al. 2021

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Over the past decades, Escherichia coli (E. coli) have acquired extensive resistance to antibiotics; especially β- lactams. This study aimed to investigate the frequency of Extended-spectrum β-lactamase (ESBL) and carbapenemase producers among E. coli isolates and their correlation with serotypes, phylogenetic background, and pathogenicity associated islands. A total of 105 E. coli strains were isolated and subjected to antimicrobial susceptibility testing against β-lactam antibiotics. All isolates showed a high resistance profile. Resistant isolates were tested for ESBL and carbapenemase production. Fifty-three and 18 isolates were positive for ESBL and carbapenemase producers, respectively. ESBL and carbapenemase genes were detected by PCR. TEM gene was the most prevalent gene among all isolates followed by SHV and CTX-M15. In carbapenemase-producers, OXA-48 and IMP were the predominant genes. Enteropathogenic E. coli (EPEC) and Enterohemorrhagic E. coli (EHEC) were the major producers of ESBL and carbapenemase, respectively as indicated by serodiagnosis. They were further assessed for the presence of pathogenicity islands (PAIs) and phylogenetic background. The most predominant DEC PAI and ExPEC PAI were HPI and IICFT073. Most clinically ESBL-producers were group D and B2 while environmentally ones were group B1 and A. On contrary, clinically carbapenemase-producers belonged to group C and D. In conclusion, our study confirms the importance of phylogenetic group D, B2, and C origin for antibiotic resistance in E. coli. Ultimately, our findings support the fact that environmental isolates contribute to the local spread of E. coli pathogenicity in Egypt and these isolates maybe serve as reservoirs for transmission of resistance.

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Section: Plos Onementioning

confidence: 90%

Genetic characterization of extended-spectrum β-Lactamase- and carbapenemase-producing Escherichia coli isolated from Egyptian hospitals and environments

et al. 2021

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“…Our finding agrees with studies conducted in Gambia, 41 Burkina Faso, 33 Ghana, 34 Saudi Arabia, 42 Israel, 43 Poland, 44 Sierra Leone, 45 and Lebanon. 46 This indicates ESBL-producing Enterobacteriaceae were rapidly emerging in developing and developed countries. In this study, ESBL-producing E. coli and K. pneumoniae were not only resistant to third-generation cephalosporins but also to other non-β lactams groups of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Fecal Carriage Rate of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Among Apparently Health Food Handlers in Dilla University Student Cafeteria

Diriba

Awulachew

Tekele

et al. 2020

IDR

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Background The rapid spread of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae among food handlers is a public health concern and has become a serious world threat. Klebsiella pneumoniae and Escherichia coli are the predominant ESBL-producing Enterobacteriaceae. This study aimed to assess the magnitude of ESBL producing K. pneumoniae and E. coli isolated from the stool of food handlers in the Dilla University Student Cafeteria. Methods A cross-sectional study was conducted from November to September 2018/2019. A total of 220 stool samples were cultured on MacConkey agar and a series of biochemical tests were performed for the identification of bacterial species. Double-disk synergy method was used for the detection of ESBL-producing strains. Results The rate of fecal carriage of E. coli and K. pneumoniae among the food handlers of the current study was 66.4%, of which 81.5% was E. coli , while 18.5% was K. pneumoniae . Of the total bacterial isolates, 25.3% were confirmed to be positive for ESBL production, of which 19.9% was E. coli , while 5.5% was K. pneumoniae . ESBL-producing fecal carriage showed high resistance to aztreonam, cefotaxime, ceftriaxone, cefuroxime, and ceftazidime, with resistance rates ranging from 88% to 100%, while the lowest resistance rate was observed against imipenem accounted <26.3%. In this study, 91.9% of ESBL-producers were co-resistant to β-lactams antibiotics plus at least one of the non-β-lactam antibiotics. ESBL carriers were more common among food handlers who used antibiotics repeatedly, drunk unpasteurized milk, and ate raw meat. Conclusion The magnitude of ESBL-producing E. coli and K. pneumoniae fecal carriage is alarmingly high and a threat to human health. MDR to third-generation cephalosporins and non-β-lactam antibiotics is more common among ESBL producers. Detecting ESBL-producing organisms has a remarkable importance in clinical decision-making. Hence, a coordinated effort should be implemented for the prevention and control of the disease.

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“…Strain EBJ003 belongs to ST405 and a different phylogenetic group D, suggesting distinct clonal dissemination from others. ST405 is a high-risk clone found in diverse hosts and it carries the bla NDM and bla CTX-M genes [35]. The bla NDM-5 -positive strains in this study were associated with ESBL/AmpC genes or CTX-M-types, demonstrating their ability to acquire different resistance genes.…”

Section: Discussionmentioning

confidence: 63%

Dissemination of Blandm-5 in Escherichia Coli via the Incx3 Plasmid From Different Regions in China

Hu¹,

Yang²,

Dong³

et al. 2021

Preprint

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Background: Recently, the spread of NDM-5-producing Escherichia coli has become a severe challenge in clinical therapy, which necessitates reliable detection and surveillance methods. However, limited information is available regarding the prevalence and dissemination of the blaNDM-5 gene in Escherichia coli in China. Therefore, we investigated the dissemination of the blaNDM-5 gene in carbapenem-resistant Escherichia coli isolates from different regions in China.Methods: A total of 1,180 carbapenem-resistant enterobacteriaceae strains were obtained from patients admitted to the 20 sentinel hospitals in eight cities. Strains positive for blaNDM-5 were detected using the Vitek 2 compact system, 16S rRNA gene sequencing, PCR, the S1-pulsed-field gel electrophoresis assay, and Southern blot hybridization. The horizontal-transfer capability of the blaNDM gene was assessed by filter mating with a standard E. coli J53 azide-resistant strain as the recipient. Genotyping, susceptibility testing, and whole genome sequencing were performed. Results: Seven strains of blaNDM-5-positive E.coli was detected in 1180 clinical strains from different regions in China. The blaNDM-5-carrying strains showed resistance to multiple tested antibiotics and belonged to two widespread sequence types, ST167 and ST405. Antimicrobial resistance genes including blaCTX-M, blaOXA, blaCMY, and two novel blaTEM variants (blaTEM-230 and blaTEM-231) were also identified. Southern blotting located the blaNDM-5 gene on 46-kb IncX3 plasmids in all isolates, which showed only two single nucleotide differences between EJN003 and the other strains. Conclusions: This study further confirms the increasing occurrence of blaNDM-5-carrying IncX3 plasmids and the dissemination of carbapenem resistance in E. coli isolates via the plasmid from different parts in China, which warrants stringent surveillance and control measures.

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Molecular characterization of Carbapenem resistant Escherichia coli recovered from a tertiary hospital in Lebanon

Cited by 46 publications

References 56 publications

Genetic characterization of extended-spectrum β-Lactamase- and carbapenemase-producing Escherichia coli isolated from Egyptian hospitals and environments

Genetic characterization of extended-spectrum β-Lactamase- and carbapenemase-producing Escherichia coli isolated from Egyptian hospitals and environments

<p>Fecal Carriage Rate of Extended-Spectrum Beta-Lactamase-Producing <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> Among Apparently Health Food Handlers in Dilla University Student Cafeteria</p>

Dissemination of Blandm-5 in Escherichia Coli via the Incx3 Plasmid From Different Regions in China

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