Molecular characterization of carbapenem-resistant Klebsiella pneumoniae in a tertiary university hospital in Turkey

Alp, Emine; Perçin, Duygu; Çolakoğlu, Şule; Durmaz, Süleyman; Kürkcü, C.A.; Ekincioğlu, Pınar; Güneş, Tamer

doi:10.1016/j.jhin.2013.03.002

Cited by 48 publications

(36 citation statements)

References 7 publications

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“…Recently we reported the first NDM-1 producing CRKP isolates, expressed from 4.3% of isolates obtained from pediatric units. However, OXA-48 type carbapenemases are still the predominant mechanism of resistance (91.5%) in our institution [10]. This recent study has also shown that there were three main clones causing several outbreaks clustered in our pediatric units [10].…”

Section: The Studymentioning

confidence: 64%

“…However, OXA-48 type carbapenemases are still the predominant mechanism of resistance (91.5%) in our institution [10]. This recent study has also shown that there were three main clones causing several outbreaks clustered in our pediatric units [10]. The open-plan layout of the pediatric units, with limited single rooms, excessive number of patients, high workload, inadequate staffing levels are the major limitations to colonization control among patients.…”

Section: The Studymentioning

confidence: 80%

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Risk factors for carbapenem resistant Klebsiella pneumoniae rectal colonization in pediatric units

Ulu-Kılıç

Alp

Perçin

et al. 2014

J Infect Dev Ctries

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Section: The Studymentioning

confidence: 64%

Section: The Studymentioning

confidence: 80%

Risk factors for carbapenem resistant Klebsiella pneumoniae rectal colonization in pediatric units

Ulu-Kılıç

Alp

Perçin

et al. 2014

J Infect Dev Ctries

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“…In Turkey, the first NDM-1 positive K. pneumoniae isolate was reported from the blood culture of a child patient in 2011 (34). In 2013, the study performed on 94 carbapenem-resistant K. pneumoniae isolates recovered from rectal swab samples of inpatients reported that 4.3% of the isolates produced only NDM-1, and 1% produced both blaOXA-48 and NDM-1 (35). Currently, a NDM-1 positive K. pneumoniae epidemic was described in a tertiary hospital and 2 of the isolates also harbored the blaOXA-48 (36).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Çelikbilek¹,

Ünaldı²,

Kırca³

et al. 2017

Jundishapur J Microbiol

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Background: Carbapenem-resistant Klebsiella pneumoniae isolates can spread among the hospitalized patients and result in serious infections and increase mortality. Molecular studies provide useful information about resistance mechanisms and crosstransmission among the resistant isolates. Objectives: The current study aimed at investigating phenotypic and molecular characteristics of the carbapenem resistance and clonal relationship among the K. pneumoniae isolates recovered from infection sites and rectal swabs of the patients hospitalized in a tertiary hospital. Methods: Resistance to carbapenems was investigated by disc-diffusion and E-test methods. Modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA)-combine disc (ECD) methods were performed to determine carbapenemases. Carbapenemase-encoding genes including blaOXA-48, blaNDM-1, blaKPC, blaIMP, and blaVIM were investigated by multiplex polymerase chain reaction (PCR). Clonal relationship among the isolates was determined by the pulsed-field gel electrophoresis (PFGE). Results: Carbapenem resistance was observed in 93 (5.8%) of the 1605 K. pneumoniae isolates. Majority (n = 66, 71%) of the resistant isolates were recovered from the patients in intensive care units (ICUs). Almost all carbapenem-resistant K. pneumoniae isolates (n = 91, 97.8%) were positive with MHT. Only 6 (6.5%) of the resistant isolates were positive with ECD method. The blaOXA-48 and blaNDM-1 were found in 90.3% (n = 84) and 6.5% (n = 6) of the resistant isolates, respectively. The pulsed-field gel electrophoresis yielded

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“…82 OXA-48-producing isolates were first described in and then spread from Turkey where frequent nosocomial outbreaks were reported. [101][102][103][104][105] These isolates have now been disseminated to many European countries. 82 OXA-48 producing K. pneumoniae strains have been reported in many Middle Eastern and African countries, but rarely from North and South Americas.…”

Section: Klebsiella Pneumoniaementioning

confidence: 99%

Epidemiology of antimicrobial resistance in bloodstream infections

2016

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Antimicrobial resistance in bacterial pathogens is a worldwide challenge leading high morbidity and mortality in clinical settings. Multidrug resistant patterns in gram-positive and -negative bacteria have resulted in difficult-to-treat or even untreatable infections with conventional antimicrobials. Since the early identification of causative microorganisms and their antimicrobial susceptibility patterns in patients with bacteremia and other serious infections is lacking in many healthcare institutions, broad spectrum antibiotics are liberally and mostly unnecessarily used. Such practice has, in turn, caused dramatic increases in emerging resistance and when coupled with poor practice of infection control, resistant bacteria can easily be disseminated to the other patients and the environment. Thus, availability of updated epidemiological data on antimicrobial resistance in frequently encountered bacterial pathogens will be useful not only for deciding on empirical treatment strategies, but also devising an effective antimicrobial stewardship program in hospitals.

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Molecular characterization of carbapenem-resistant Klebsiella pneumoniae in a tertiary university hospital in Turkey

Cited by 48 publications

References 7 publications

Risk factors for carbapenem resistant Klebsiella pneumoniae rectal colonization in pediatric units

Risk factors for carbapenem resistant Klebsiella pneumoniae rectal colonization in pediatric units

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Epidemiology of antimicrobial resistance in bloodstream infections

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