Molecular Characterization of Carbapenemase Producing Acinetobacter Baumannii and Pseudomonas aeruginosa from Tertiary Care Hospitals in Mecca - Saudi Arabia

Alhumaidy, A; Alahmadi, R; Eisa, S; Alotaibi, Meshal S.; Filfilan, S; Alzayer, Maha; Okdah, Liliane; Doumith, Michel; Taha, Omar A.; Albishri, B; Mulana, A; Alshelgani, A; Alghoribi, Majed F.; Alzeyadi, Zeyad Abdullah

doi:10.1016/j.jiph.2020.01.079

Cited by 2 publications

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“…The transmission of antibiotic-resistant strains of Enterobacteriaceae is a significant concern, and the large volume of human movement (pilgrim, tourism, work) within and outside the Gulf region is a major risk factor for their spread. These strains have been documented in several studies in various regions of Saudi Arabia [ 14 , 15 , 16 , 17 , 18 , 19 ]. There is a lack of data on the prevalence and patterns of antimicrobial resistant bacteria in hospital and municipal wastewater in Saudi Arabia.…”

Section: Introductionmentioning

confidence: 97%

Antimicrobial Resistance and β-Lactamase Production in Clinically Significant Gram-Negative Bacteria Isolated from Hospital and Municipal Wastewater

2023

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Hospital and municipal wastewater contribute to the spread of antibiotic-resistant bacteria and genes in the environment. This study aimed to examine the antibiotic resistance and β-lactamase production in clinically significant Gram-negative bacteria isolated from hospital and municipal wastewater. The susceptibility of bacteria to antibiotics was tested using the disk diffusion method, and the presence of extended-spectrum β-lactamases (ESBL) and carbapenemases was determined using an enzyme inhibitor and standard multiplex PCR. Analysis of antimicrobial resistance of total bacterial strains (n = 23) revealed that most of them were resistant to cefotaxime (69.56%), imipenem (43.47%), meropenem (47.82%) and amoxicillin-clavulanate (43.47%), gentamicin (39.13%), cefepime and ciprofloxacin (34.78%), trimethoprim-sulfamethoxazole (30.43%). A total of 8 of 11 phenotypically confirmed isolates were found to have ESBL genes. The blaTEM gene was present in 2 of the isolates, while the blaSHV gene was found in 2 of the isolates. Furthermore, the blaCTX-M gene was found in 3 of the isolates. In one isolate, both the blaTEM and blaSHV genes were identified. Furthermore, of the 9 isolates that have been phenotypically confirmed to have carbapenemase, 3 were confirmed by PCR. Specifically, 2 isolates have the blaOXA-48 type gene and 1 have the blaNDM-1 gene. In conclusion, our investigation shows that there is a significant rate of bacteria that produce ESBL and carbapenemase, which can promote the spread of bacterial resistance. Identifying ESBL and carbapenemase production genes in wastewater samples and their resistance patterns can provide valuable data and guide the development of pathogen management strategies that could potentially help reduce the occurrence of multidrug resistance.

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Section: Introductionmentioning

confidence: 97%

Antimicrobial Resistance and β-Lactamase Production in Clinically Significant Gram-Negative Bacteria Isolated from Hospital and Municipal Wastewater

2023

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“…Immunologically compromised patients are also obliged to stay in high-risk intensive care units for prolonged periods of time, during which they may be submitted to endotracheal intubation and/or catheterization of the blood vessels and bladder; also, in these units, both the air and environmental surfaces are heavily contaminated. That is why burn patients are high-risk groups for infection 2 .…”

Section: Introductionmentioning

confidence: 99%

Pan-Resistance; A Rising Challenge in Burns Patients

Khan,

Shahbazi,

Imran

et al. 2023

PJMHS

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Introduction: Burn injuries by fire and hot liquids and contact with hot surfaces have been recognized as a significant and major public health problem in economically developing countries. Objectives: The main objective of the study is to find the incidence of pan-resistant strain infections, in burns Khyber teaching hospital, Peshawar. Material and methods: A retrospective observational study was conducted to determine the incidence of pan-resistant strain infections in burn patients at Khyber Teaching Hospital, Peshawar. The study was conducted over a period of 12 months, from January 1, 2022, to December 31, 2022. Khyber Teaching Hospital is a tertiary care hospital located in Peshawar, the capital city of Khyber Pakhtunkhwa province in Pakistan. Results: Table 1 presents the demographic and clinical characteristics of the study population. The table shows that the study included 100 patients with burn injuries, with a mean age of 28.5 years (SD 14.7). The majority of patients were male (70.0%) and had burn injuries covering more than 10% of their total body surface area (78.0%). The most common cause of burn injury was flame (52.0%). The mean length of hospital stay was 15.4 days. Practical Implication: This study implies and plays a role in finding the proper antibiotics which will be used for burn wounds. Conclusion: The study concludes that a significant proportion of bacterial isolates obtained from burn wound cultures were resistant to multiple antibiotics, with Acinetobacter baumannii showing a pan-resistant pattern. Keywords: Significant, Acinetobacter, Surface, Resistance, Clinical, Wound

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Molecular Characterization of Carbapenemase Producing Acinetobacter Baumannii and Pseudomonas aeruginosa from Tertiary Care Hospitals in Mecca - Saudi Arabia

Cited by 2 publications

References 0 publications

Antimicrobial Resistance and β-Lactamase Production in Clinically Significant Gram-Negative Bacteria Isolated from Hospital and Municipal Wastewater

Antimicrobial Resistance and β-Lactamase Production in Clinically Significant Gram-Negative Bacteria Isolated from Hospital and Municipal Wastewater

Pan-Resistance; A Rising Challenge in Burns Patients

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