Molecular Characterization of Carbapenemase-Producing Pseudomonas aeruginosa of Czech Origin and Evidence for Clonal Spread of Extensively Resistant Sequence Type 357 Expressing IMP-7 Metallo-β-Lactamase

Abstract

The objective of this study was to perform molecular surveillance for assessing the spread of carbapenemase-producing in Czech hospitals. One hundred thirty-six carbapenemase-producing isolates were recovered from 22 hospitals located throughout the country. Sequence type 357 (ST357) dominated ( = 120) among carbapenemase producers. One hundred seventeen isolates produced IMP-type (IMP-7 [ = 116] and IMP-1 [ = 1]) metallo-β-lactamases (MβLs), 15 produced the VIM-2 MβL, and the remaining isolates expressed the GES-5 enzyme. The -like genes were located in three main integron types, with In-p110-like being the most prevalent ( = 115). The two other IMP-encoding integrons (In1392 and In1393) have not been described previously. -carrying integrons included In59-like, In56, and a novel element (In1391). was carried by In717. Sequencing data showed that In-p110-like was associated with a Tn-like transposon inserted in genomic island LESGI-3 in the chromosome. The other integrons were also integrated into the chromosome. These findings indicated the clonal spread of ST357 , carrying the IMP-7-encoding integron In-p110, in Czech hospitals. Additionally, the sporadic emergence of producing different carbapenemase types, associated with divergent or novel integrons, punctuated the ongoing evolution of these bacteria.

High-Risk International Clones of Carbapenem-Nonsusceptible Pseudomonas aeruginosa Endemic to Indonesian Intensive Care Units: Impact of a Multifaceted Infection Control Intervention Analyzed at the Genomic Level

“…aeruginosa high-risk international clones reported around the globe that are associated with MDR profiles. Of the four, ST235 and ST357 have been extensively reported in several countries (16–19), while the other two main STs, ST823 and ST446, emerged more recently. A recent study of MDR P.…”

High frequency of the exoU+/exoS+ genotype associated with multidrug-resistant “high-risk clones” of Pseudomonas aeruginosa clinical isolates from Peruvian hospitals

“…ST235 has been associated with a poor clinical outcome in part due to its high level of antibiotic resistance and the presence of the exoU + gene 12,29 . Regarding antibiotic resistance, ST235 and ST357 are usually resistant to FQ, aminoglycosides and β-lactams 12,25,46,47 . Likewise, all the isolates belonging to these STs were at least MR, with all ST357 isolates being XDR and displaying resistance to all antibacterial agents tested except colistin.…”

Spread of Carbapenem Resistance by Transposition and Conjugation Among Pseudomonas aeruginosa

“…Since pS04 90 is a conjugative plasmid, this illustrates how MBL-encoding integrons can be mobilized and transferred between strains. Interestingly, the entire composite transposon also appears to be present in a P. aeruginosa isolate of Czech origin, i.e., strain Pae-31448cz ( Papagiannitsis et al, 2017 ). However, the severely disrupted Tn 4661 , although present in the available nucleotide sequence (Genbank accession number KY860571.1 ), was not noticed and, therefore, the possibility that entire fragment could function as a composite mobile element was not considered.…”