Molecular characterization of cathelicidin in tiger frog (Hoplobatrachus rugulosus): Antimicrobial activity and immunomodulatory activity

Chen, Jie; Lin, Youfu; Chen, Jia-Hao; Chen, Xiang; Lin, Zhihua

doi:10.1016/j.cbpc.2021.109072

Cited by 18 publications

(13 citation statements)

References 39 publications

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“…Pig cathelicidin research by Ahn et al (3) indicated the potential use of genetic testing in animal breeding. Chen et al (8) described the characteristics of tiger frog cathelicidin, confirming a similar structure of the gene precursor to that of cathelicidin genes in other species, and at the same time observing a potential strong antibacterial effect against pathogenic microorganisms. Wang et al (35) investigated avian cathelicidins found in chickens, pheasants, quails, turkeys and pigeons as potential therapeutic agents with antimicrobial properties that could replace antibiotics following more detailed research.…”

Section: Introductionmentioning

confidence: 76%

Analysis of the effect of polymorphisms within the CATHL7 gene on dairy performance parameters

Hiller¹,

Kowalewska²,

Czerniawska-Piątkowska

et al. 2023

Journal of Veterinary Research

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Introduction Antimicrobial peptides, including cathelicidins, play a significant role in farm animals, influencing animal welfare, immunity, and thus the quality of animal products. Material and Methods The study used amplification-created restriction site and PCR-restriction fragment length polymorphism to analyse single nucleotide polymorphisms of the CATHL7 gene encoding the BMAP-34 protein in cattle, at positions 2,383 G > C and 2,468 G > C. The material was collected from 279 Polish Black-and-White Holstein-Friesian dairy cows. Results There were statistically significant differences between milk performance parameters in cows with the CATHL7/HhaI and CATHL7/HinfI genotypes. In the case of the CATHL7/HhaI polymorphism, the highest milk yield and protein and lactose content and the lowest somatic cell count in milk were observed for the CC genotype, while the fat content was the highest in milk from cows with the GG genotype. In the case of the CATHL7/HinfI polymorphism, the highest protein and lactose content in milk was observed for the CC genotype. Conclusion The results were statistically significant, which suggests that the search for relationships can be continued, and that the results can be used to improve selection programmes supporting dairy farming.

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Section: Introductionmentioning

confidence: 76%

Analysis of the effect of polymorphisms within the CATHL7 gene on dairy performance parameters

Hiller¹,

Kowalewska²,

Czerniawska-Piątkowska

et al. 2023

Journal of Veterinary Research

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“…Thus, amphibian skins are an important source of wound healing-promoting peptides. ,− Although two bioactive peptides with insulin-releasing, antimicrobial, and immunomodulatory activity have been identified from the skin of H. rugulosus, − no information about wound healing-promoting peptides. Here, we have identified that the 11 residue long linear peptide TK-HR possesses a strong capacity to promote wound closure.…”

Section: Discussionmentioning

confidence: 99%

“…rugulosus has long been utilized in Chinese folk medicine to promote diuresis and detumescence, detoxify, and stop coughing . To date, only HR-CATH and Tigerinin-1R with immunomodulatory activity have been identified from its skin extracts. − In the present study, a new TK, named TK-HR, was identified from the skin of H. rugulosus, and its ability to promote wound healing was analyzed in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 89%

Peptide TK-HR from the Skin of Chinese Folk Medicine Frog Hoplobatrachus Rugulosus Accelerates Wound Healing via the Activation of the Neurokinin-1 Receptor

Wu,

Xiong,

et al. 2023

J. Med. Chem.

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“…Detection of damage in the bacterial cell membrane was determined by the LDH Release Assay Kit (Beyotime, Shanghai, China) following the study of Chen et al [ 40 ]. 1 × 10 9 CFUs of A. hydrophila was obtained by centrifugation at 5000× g for 2 min, and re-suspended into 200 μL PBS.…”

Section: Methodsmentioning

confidence: 99%

Host defence peptide LEAP2 contributes to antimicrobial activity in a mustache toad (Leptobrachium liui)

et al. 2023

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Background The liver-expressed antimicrobial peptide 2 (LEAP2) is essential in host immunity against harmful pathogens and is only known to act as an extracellular modulator to regulate embryonic development in amphibians. However, there is a dearth of information on the antimicrobial function of amphibian LEAP2. Hence, a LEAP2 homologue from Leptobrachium liui was identified, characterized, and chemically synthesized, and its antibacterial activities and mechanisms were determined. Results In this study, LEAP2 gene (Ll-LEAP2) cDNA was cloned and sequenced from the Chong’an Moustache Toad (Leptobrachium liui). The predicted amino acid sequence of Ll-LEAP2 comprises a signal peptide, a mature peptide, and a prodomain. From sequence analysis, it was revealed that Ll-LEAP2 belongs to the cluster of amphibian LEAP2 and displays high similarity to the Tropical Clawed Frog (Xenopus tropicalis)‘s LEAP2. Our study revealed that LEAP2 protein was found in different tissues, with the highest concentration in the kidney and liver of L. liui; and Ll-LEAP2 mRNA transcripts were expressed in various tissues with the kidney having the highest mRNA expression level. As a result of Aeromonas hydrophila infection, Ll-LEAP2 underwent a noticeable up-regulation in the skin while it was down-regulated in the intestines. The chemically synthesized Ll-LEAP2 mature peptide was selective in its antimicrobial activity against several in vitro bacteria including both gram-positive and negative bacteria. Additionally, Ll-LEAP2 can kill specific bacteria by disrupting bacterial membrane and hydrolyzing bacterial gDNA. Conclusions This study is the first report on the antibacterial activity and mechanism of amphibian LEAP2. With more to uncover, the immunomodulatory functions and wound-healing activities of Ll-LEAP2 holds great potential for future research.

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Molecular characterization of cathelicidin in tiger frog (Hoplobatrachus rugulosus): Antimicrobial activity and immunomodulatory activity

Cited by 18 publications

References 39 publications

Analysis of the effect of polymorphisms within the CATHL7 gene on dairy performance parameters

Analysis of the effect of polymorphisms within the CATHL7 gene on dairy performance parameters

Peptide TK-HR from the Skin of Chinese Folk Medicine Frog Hoplobatrachus Rugulosus Accelerates Wound Healing via the Activation of the Neurokinin-1 Receptor

Host defence peptide LEAP2 contributes to antimicrobial activity in a mustache toad (Leptobrachium liui)

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