Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)

Zhao, Wei; Chen, Liqiao; Zhang, F.; Wu, Ping; Li, Erchao; Qin, Jianguang

doi:10.4238/2013.october.30.6

Cited by 22 publications

(14 citation statements)

References 36 publications

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“…In the current study, we found high occurrence of Vg and VgR in ovary of P. clarkii , and RT-PCR demonstrated that the expression level in ovary is significantly higher than in testis which provided some clues to further elucidate the function to ovary development of P. clarkii ( Table S1 ). Cathepsins belong to a family of proteases that cleave other proteins and are ubiquitously present in almost all organisms [41] , and involved in ovary maturation and embryo development [14] , [42] . There are two kind of cathepsins: cathepsin C and L in our study, and were largely expressed in ovary libraries.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of Red Swamp Crawfish Procambarus clarkii Reveals Genes Involved in Gonadal Development

Jiang

Xing

Lü³

et al. 2014

PLoS ONE

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BackgroundThe red swamp crawfish, Procambarus clarkii, has become one of the most economically important cultured species in China. Currently, little is known about the gonadal development of this species. Isolation and characterization of genes are an initial step towards understanding gonadal development of P. clarkii.ResultsUsing the 454 pyrosequencing technology, we obtained a total of 1,134,993 high quality sequence reads from the crawfish testis and ovary libraries. We aimed to identify different genes with a potential role in gonad development. The assembly formed into 22,652 isotigs, distributed by GO analysis across 55 categories in the three ontologies, ‘molecular function’, ‘cellular component’, and ‘biological processes’. Comparative transcript analysis showed that 1,720 isotigs in the ovary were up-regulated and 2138 isotigs were down-regulated. Several gonad development related genes, such as vitellogenin, cyclin B, cyclin-dependent kinases 2, Dmc1 and ubiquitin were identified. Quantitative real-time PCR verified the expression profiles of 14 differentially expressed genes, and confirmed the reliability of the 454 pyrosequencing.ConclusionsOur findings provide an archive for future research on gonadal development at a molecular level in P. clarkii and other crustacean. This data will be helpful to develop new ideas for artificial regulation of the reproductive process in crawfish aquaculture.

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Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of Red Swamp Crawfish Procambarus clarkii Reveals Genes Involved in Gonadal Development

Jiang

Xing

Lü³

et al. 2014

PLoS ONE

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“…Although PTGR1 is involved in the ovary development in the shrimp Penaeus monodon [80], it was not preferentially expressed in the ovaries of Palaemon serratus . Cathepsins have been also related with ovarian development in some crustaceans [22, 81, 82]. Cathepsin D is a needed protein for the formation of the yolk in vertebrates [83] and its gene was the only cathepsin gene up-regulated in the ovary of P. serratus .…”

Section: Discussionmentioning

confidence: 99%

De novo gonad transcriptome analysis of the common littoral shrimp Palaemon serratus: novel insights into sex-related genes

et al. 2019

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BackgroundThe common littoral shrimp Palaemon serratus is an economically important decapod resource in some European communities. Aquaculture practices prevent the genetic deterioration of wild stocks caused by overfishing and at the same time enhance the production. The biotechnological manipulation of sex-related genes has the proved potential to improve the aquaculture production but the scarcity of genomic data about P. serratus hinders these applications. RNA-Seq analysis has been performed on ovary and testis samples to generate a reference gonadal transcriptome. Differential expression analyses were conducted between three ovary and three testis samples sequenced by Illumina HiSeq 4000 PE100 to reveal sex-related genes with sex-biased or sex-specific expression patterns.ResultsA total of 224.5 and 281.1 million paired-end reads were produced from ovary and testis samples, respectively. De novo assembly of ovary and testis trimmed reads yielded a transcriptome with 39,186 transcripts. The 29.57% of the transcriptome retrieved at least one annotation and 11,087 differentially expressed genes (DEGs) were detected between ovary and testis replicates. Six thousand two hundred seven genes were up-regulated in ovaries meanwhile 4880 genes were up-regulated in testes. Candidate genes to be involved in sexual development and gonadal development processes were retrieved from the transcriptome. These sex-related genes were discussed taking into account whether they were up-regulated in ovary, up-regulated in testis or not differentially expressed between gonads and in the framework of previous findings in other crustacean species.ConclusionsThis is the first transcriptome analysis of P. serratus gonads using RNA-Seq technology. Interesting findings about sex-related genes from an evolutionary perspective (such as Dmrt1) and for putative future aquaculture applications (Iag or vitellogenesis genes) are reported here. We provide a valuable dataset that will facilitate further research into the reproductive biology of this shrimp.

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“…The activity of carboxypeptidases А and В, as well as that of leucine aminopeptidase (Dendinger, 1987;Glass & Stark, 1995) was detected also. In various shrimp tissues cDNA coding cathepsin L (Duan et al, 2013;Zhao et al, 2013), cathepsin B (Stephens et al, 2012;Li et al, 2013) and cathepsin С (Wang et al, 2012) were revealed. These data suggest that chironomid larvae may possess a wide range of peptidases.…”

Section: Discussionmentioning

confidence: 99%

Temperature characteristics of peptidase in chironomid larvae, potential fish prey, at various pH values

Кузьмина

Chornaya²,

Skvortsova

et al. 2018

Biosys. divers.

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The temperature dependence of casein- and hemoglobinlytic peptidases functioning in the whole organism of chironomid larvae Chironomus plumosus, food objects of adult benthophages and juvenile fish of various ecological groups, was studied within the temperature range of 0–70 ºС at different рН values (3.0, 5.0 and 7.4). The method of mixed samples was used to determine the activity and characteristics of enzymes. Homogenates of previously crushed and carefully mixed dozens of larvae were used as enzymatically active preparations. Activity of peptidases was assayed by the increase in tyrosine concentration using the Folin-Ciocalteu reagent. It is shown that the activity of peptidases that function in the tissues of chironomid larvae depends to a considerable extent on temperature and рН, but the pH has a smaller effect on the activity and the temperature dependence of casein- and hemoglobin-lytic peptidases than temperature. The temperature optimum of the studied peptidases of chironomid larvae corresponds to 40 ºС. The Q10 values in the zone of vital temperatures are slightly changed. They are, as a rule, increased in the zone of 30–40 ºС, and are sharply decreased in the zone of high temperatures. The values of activation energy of the process of hydrolysis of casein and hemoglobin in the zone of low and high temperatures are different. The Еact values of the process of hydrolysis of casein and hemoglobin at a temperature not exceeding 20 ºС are usually below those in the zone of higher temperatures (except for hemoglobin-lytic peptidases at pH 5.0). The data obtained indicate a significant effect of pH not only on the activity, but also on the temperature characteristics of peptidases that function in the body of chironomid larvae. Differences in the characteristics of casein- and hemoglobin-lytic peptidases in chironomid larvae at different temperatures and pH can influence the digestion in benthophages and fry of all fish species.

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Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)

Cited by 22 publications

References 36 publications

Transcriptome Analysis of Red Swamp Crawfish Procambarus clarkii Reveals Genes Involved in Gonadal Development

Transcriptome Analysis of Red Swamp Crawfish Procambarus clarkii Reveals Genes Involved in Gonadal Development

De novo gonad transcriptome analysis of the common littoral shrimp Palaemon serratus: novel insights into sex-related genes

Temperature characteristics of peptidase in chironomid larvae, potential fish prey, at various pH values

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