Molecular characterization of Caulobacter crescentus mutator strains

Martins-Pinheiro, Marinalva; Oliveira, Anderson Rodrigues de; Valencia, Alexy Orozco; Fernandez-Silva, Frank S.; Silva, Larissa G.; Lopes-Kulishev, Carina O.; Italiani, Valéria C. S.; Marques, Marilis V.; Menck, Carlos Frederico Martins; Galhardo, Rodrigo S.

doi:10.1016/j.gene.2017.05.038

Cited by 13 publications

(19 citation statements)

References 44 publications

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“…In a search for C. crescentus strains with increased spontaneous mutagenesis, we have previously identified a mutY insertion mutant (Martins-Pinheiro et al 2017). Nevertheless, the Rif R mutation rate of this strain is only a few-fold increase in comparison to the parental strain, which is different from previous observations in E. coli, where mutY inactivation causes more than a 20-fold increase in spontaneous mutant frequencies (Nghiem et al 1988;Fowler et al 2003).…”

Section: Introductioncontrasting

confidence: 82%

“…Nevertheless, we observed that the mutator phenotype displayed by this strain is modest when compared to E. coli data from the literature (Martins-Pinheiro et al 2017). Nevertheless, we observed that the mutator phenotype displayed by this strain is modest when compared to E. coli data from the literature (Martins-Pinheiro et al 2017).…”

Section: Lack Of Mutm and Muty Confers A Mild Mutator Phenotype To Cmentioning

confidence: 46%

“…In vitro data with Endo III homologs from E. coli and humans showed that these enzymes possess activity against some substrates containing this lesion (Matsumoto et al 2001). Although there still remains the formal possibility that other unknown repair pathways present in C. crescentus could help to prevent 8-oxodG mutagenesis and act as a backup for the GO system, this is unlikely given that no other mutators besides the canonical repair genes were found in C. crescentus (Martins-Pinheiro et al 2017). Although there still remains the formal possibility that other unknown repair pathways present in C. crescentus could help to prevent 8-oxodG mutagenesis and act as a backup for the GO system, this is unlikely given that no other mutators besides the canonical repair genes were found in C. crescentus (Martins-Pinheiro et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Genetic interaction analysis of spontaneous and MB-induced mutagenesis indicates that the Endo III enzymes of C. crescentus are not involved in protection against 8-oxodG mutagenesis. Although there still remains the formal possibility that other unknown repair pathways present in C. crescentus could help to prevent 8-oxodG mutagenesis and act as a backup for the GO system, this is unlikely given that no other mutators besides the canonical repair genes were found in C. crescentus (Martins-Pinheiro et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, we have identified a mutY mutant in a screening for mutators in C. crescentus. Nevertheless, we observed that the mutator phenotype displayed by this strain is modest when compared to E. coli data from the literature (Martins-Pinheiro et al 2017). To further explore the biological effects of spontaneously formed 8-oxoG in C. crescentus, we constructed a mutY mutM double mutant, and compared the spontaneous mutation rates to that of an E. coli strain with the same phenotype, using the same mutagenesis marker (Rif R mutations in rpoB).…”

Section: Lack Of Mutm and Muty Confers A Mild Mutator Phenotype To Cmentioning

confidence: 97%

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Contribution of GO System Glycosylases to Mutation Prevention in Caulobacter crescentus

Fernández-Silva

Schulz

Alves

et al. 2019

Environ and Mol Mutagen

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8‐oxo‐7,8‐dihydroguanine, commonly referred to as 8‐oxoG, is considered one of the most predominant oxidative lesions formed in DNA. Due to its ability to pair with adenines in its syn configuration, this lesion has a strong mutagenic potential in both eukaryotes and prokaryotes. Escherichia coli cells are endowed with the GO system, which protects them from the mutagenic properties of this lesion when formed both in cellular DNA and the nucleotide pool. MutY and MutM (Fpg) DNA glycosylases are crucial components of the GO system. A strong mutator phenotype of the Escherichia coli mutM mutY double mutant underscores the importance of 8‐oxoG repair for genomic stability. Here, we report that in Caulobacter crescentus, a widely studied alpha‐proteobacterium with a GC‐rich genome, the combined lack of MutM and MutY glycosylases produces a more modest mutator phenotype when compared to E. coli. Genetic analysis indicates that other glycosylases and other repair pathways do not act synergistically with the GO system for spontaneous mutation prevention. We also show that there is not a statistically significant difference in the spontaneous levels 8‐oxodGuo in E. coli and C. crescentus, suggesting that other yet to be identified differences in repair or replication probably account for the differential importance of the GO system between these two species. Environ. Mol. Mutagen. 61:246–255, 2020. © 2019 Wiley Periodicals, Inc.

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Section: Introductioncontrasting

confidence: 82%

Section: Lack Of Mutm and Muty Confers A Mild Mutator Phenotype To Cmentioning

confidence: 46%