2011
DOI: 10.5897/ajmr11.708
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Molecular characterization of Citrus tristeza virus strains in Peninsular Malaysia

Abstract: Sixty Malaysian Citrus tristeza virus (CTV) isolates were characterized by bi-directional polymerase chain reaction (BD-PCR) and restriction fragment length polymorphism (RFLP) analysis of their coat protein (CP) gene. In BD-PCR analysis, 392-bp fragments were amplified from seven isolates. The other 53 isolates produced only 320-bp fragments. RFLP patterns of RT-PCR products of CP gene digested with HinfI restriction enzyme were similar to I-IV, VI-VII and two new groups. Isolates AMK1, AMJ12, AMT38, AMT39 an… Show more

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