Molecular characterization of classic human astrovirus in eastern China, as revealed by environmental sewage surveillance

Zhou, Nan; Lin, Xiaojuan; Wang, Suting; Wang, Haiyan; Bi, Zhuanfang; Wang, Pei; Chen, Peng; Tao, Zexin; Song, Lizhi; Song, Yue; Xu, Aiqiang

doi:10.1111/jam.13109

Cited by 13 publications

(7 citation statements)

References 38 publications

(40 reference statements)

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“…In addition to Italy, the HAstV3/HAstV2 was also reported from Russia ( Babkin et al, 2014 ) and Kenya ( Wolfaardt et al, 2011 ). The HAstV4/HAstV5 was reported from South Africa ( Taylor et al, 2001 ) and China ( Zhou et al, 2016 ). In addition, the HAstV1/HAstV5, HAstV2/HAstV5, and HAstV1/HAstV2 were also reported from China ( Zhou et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

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Emergence of Multiple Novel Inter-Genotype Recombinant Strains of Human Astroviruses Detected in Pediatric Patients With Acute Gastroenteritis in Thailand

et al. 2021

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Objective: Human astrovirus (HAstV) is recognized as an important cause of acute gastroenteritis in children. Recombination between different genotypes of HAstV can contribute to diversity and evolution of the virus. This study aimed to investigate the emergence of HAstV recombinant strains in pediatric patients hospitalized with acute gastroenteritis in Chiang Mai, Thailand, spanning 2011–2020.Methods: A total of 92 archival HAstV strains collected from pediatric patients with acute gastroenteritis during 2011–2020 were further characterized to identify the recombinant strains. The ORF1b and ORF2 junction region of each strain was amplified and sequenced. The obtained sequences were analyzed in comparison with the reference sequences retrieved from GenBank database. Their genotypes were assigned using MEGA X software based on the partial ORF1b (RdRp) and ORF2 (capsid) regions, and the recombination breakpoints of recombinant strains were determined by SimPlot and RDP4 analyses.Results: Five inter-genotype recombinant strains with three recombination patterns of ORF1b/ORF2 of classic HAstV, HAstV8/HAstV1, HAstV8/HAstV3, and HAstV3/HAstV2, were detected. The recombination breakpoints of all strains were located at the 3′-end region of ORF1b close to the ORF1b/ORF2 junction.Conclusion: Several novel inter-genotype recombinant strains of classic HAstV genotypes were detected in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand, during the period of 10 years from 2011 to 2020.

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Section: Discussionmentioning

confidence: 99%

“…The HAstV4/HAstV5 was reported from South Africa ( Taylor et al, 2001 ) and China ( Zhou et al, 2016 ). In addition, the HAstV1/HAstV5, HAstV2/HAstV5, and HAstV1/HAstV2 were also reported from China ( Zhou et al, 2016 ). Furthermore, the HAstV2/HAstV8 was reported from South Korea ( Ha et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Emergence of Multiple Novel Inter-Genotype Recombinant Strains of Human Astroviruses Detected in Pediatric Patients With Acute Gastroenteritis in Thailand

et al. 2021

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“…2003; Zhou et al . 2016). In either reaction, 5 μ l of extracted RNA was added and the final reaction volume was 50 μ l. Both reaction conditions were: 50°C for 30 min followed by 94°C for 2 min plus 35 cycles of 94°C for 10 s, 50°C for 30 s and 72°C for 30 s with a final extension step of 72°C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Continuous detection of norovirus and astrovirus in wastewater in a coastal city of China in 2014–2016

Lin

Zou

Liu

et al. 2021

Lett Appl Microbiol

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Norovirus (NoV) and human astrovirus (HAstV) are important causative agents of acute gastroenteritis in children and adults. They are comprised of multiple genotypes and attention should be paid to genotype changes or emergence of new genetic variants. To study the prevalence and diversity of NoV GI, GII, and HAstV circulating in eastern China, we conducted a three‐year environmental surveillance in a coastal city of Yantai. Thirty‐six sewage samples were collected, processed, and examined for the presence of viral genomes by PCR. The results showed that NoV GI, GII, and HAstV were detected in all 36 samples. Six NoV GI genotypes, 11 NoV GII genotypes, and 5 HAstV serotypes were identified; GI.6, GII.17, and HAstV‐5 were the most prevalent types, respectively. Persistent existence of NoV GII.17 Kawasaki 308 variant was observed during whole study period. Phylogenetic analysis reflected multiple transmission lineages in local population for both viruses. Our results reflect continuous presence of enteric viruses in sewage, improve our understanding on their molecular epidemiology, and demonstrate surveillance on sewage is an effective approach in understanding the local circulation of enteric viruses.

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“…Some articles assessed the presence of poliovirus, [69][70][71][72] either wildtype or vaccine-derived, including potential neurovirulence of vaccine-derived poliovirus. [73][74][75] Many articles documented the diversity of non-polio enteroviruses found in wastewater, 73,[76][77][78][79][80][81][82][83][84][85][86][87][88][89][90][91][92] with a variety of focuses including rotavirus, [93][94][95][96][97] norovirus, 88,92,[98][99][100] astrovirus, 101,102 polyomavirus, 103 Saffold virus, 104 hepatitis A virus, 85 hepatitis E virus, 105 mastadenovirus, 106 Aichi virus, 107 and human bocavirus. 107 Surveillance of Giardia and/or Cryptosporidium was also documented.…”

Section: Figure 2 Global Distribution Of Studies Of Wastewater Surveillance For Infectious Diseasementioning

confidence: 99%

Wastewater surveillance for infectious disease: a systematic review

Kilaru

Hill

Anderson³

et al. 2021

Preprint

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Wastewater surveillance of SARS-CoV-2 has shown to be a valuable source of information regarding SARS-CoV-2 transmission and COVID-19 cases. Though the method has been used for several decades to track other infectious diseases, there has not been a comprehensive review outlining all of the pathogens surveilled through wastewater. The aim of this study is to identify what infectious diseases have been previously studied via wastewater surveillance prior to the COVID-19 Pandemic and identify common characteristics between the studies, as well as identify current gaps in knowledge. Peer-reviewed articles published as of August 1, 2020 that examined wastewater for communicable and infectious human pathogens on 2 or more occasions were included in the study. Excluded from this list were all reviews and methods papers, single collection studies, and non-human pathogens. Infectious diseases and pathogens were identified in studies of wastewater surveillance, as well as themes of how wastewater surveillance and other measures of disease transmission were linked. This review did not include any numerical data from individual studies and thus no statistical analysis was done. 1005 articles were identified but only 100 were included in this review after applying the inclusion criteria. These studies came from 38 countries with concentration in certain countries including Italy, Israel, Brazil, Japan, and China. Twenty-five separate pathogen families were identified in the included studies, with the majority of studies examining pathogens from the family Picornaviridae, including polio and non-polio enteroviruses. Most studies of wastewater surveillance did not link what was found in the wastewater to other measures of disease transmission. Among those studies that did compare wastewater surveillance to other measures of disease transmission the value observed was dependent upon pathogen and varied by study. Wastewater surveillance has historically been used to assess water-borne and fecal-orally transmitted pathogens causing diarrheal disease. However, numerous other types of pathogens have been surveilled using wastewater and wastewater surveillance should be considered as a potential tool for many infectious diseases. Wastewater surveillance studies can be improved by incorporating other measures of disease transmission at the population-level including disease incidence and hospitalizations.

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Molecular characterization of classic human astrovirus in eastern China, as revealed by environmental sewage surveillance

Abstract: The results demonstrate that sewage analysis is a useful approach in surveillance HAstVs in the context of limited clinical data.

Cited by 13 publications

References 38 publications

Emergence of Multiple Novel Inter-Genotype Recombinant Strains of Human Astroviruses Detected in Pediatric Patients With Acute Gastroenteritis in Thailand

Emergence of Multiple Novel Inter-Genotype Recombinant Strains of Human Astroviruses Detected in Pediatric Patients With Acute Gastroenteritis in Thailand

Continuous detection of norovirus and astrovirus in wastewater in a coastal city of China in 2014–2016

Wastewater surveillance for infectious disease: a systematic review

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