Molecular Characterization of Clinical Strains of Extended-Spectrum Βeta-Lactamases-Producing Klebsiella Pneumoniae Isolated in A Tertiary Hospital in Dakar-Senegal

Dossouvi, Komla Mawunyo; Ba, Bissoume Sambe

doi:10.26502/ami.93650099

Cited by 2 publications

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“…Almost half of the samples were pus (53.05%), followed by urine (40.69% [35,36] also reported high Enterobacteriaceae resistance rates to ciprofloxacin and low resistance rates to carbapenems and Amikacin. The high rate of resistance to fluoroquinolones in Enterobacteriaceae compromises the use of this class of antibiotics in probabilistic antibiotic therapy.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Profiles of Bacteria Isolated from Patients Hospitalized at the Sylvanus Olympio University Teaching Hospital in Lomé, Togo

Dossim,

Bawe,

Dossouvi

et al. 2024

MRJI

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Aims: Bacteria isolated from hospitalized patients are often responsible for healthcare-associated infections (HAI), particularly in developing countries. Thus, this study aimed to determine the antibiotic resistance profiles of bacteria isolated from patients hospitalized at the Sylvanus Olympio University Teaching Hospital (CHUSO) in Lomé, Togo. Study Design: This was a descriptive cross-sectional study carried out on laboratory data collected from January 1, 2018, to December 31, 2019. Methodology: The Kirby-Bauer disc diffusion method was used for antibiotic susceptibility testing and the results were interpreted according to the guidelines of the Antibiogram Committee of the French Society of Microbiology (CA-SFM, 2018). Results: A total of 639 samples were collected, including mainly pus (n = 339; 53.1%) and urine (n = 260; 40.7%). The samples were mainly from pediatrics (n=107; 16.7%), intensive care units (n=73; 11.4%) and surgical emergencies (n=72; 11.3%). A total of 698 bacteria were isolated, including mainly Escherichia coli (n=247, 35.4%), Staphylococcus aureus (n=123, 17.6%), and Klebsiella pneumoniae (n=114, 16.3%). Enterobacteriaceae strains were resistant to almost all antibiotics tested, except amikacin and ertapenem, which had respective resistance rates of 2.8% and 8.4%. None of the P. aeruginosa strains were resistant to piperacillin-tazobactam. whereas 37.9% were resistant to imipenem. Among A. baumannii strains, 4.2% and 22.9% were respectively resistant to amikacin and imipenem, whereas 56.3% were resistant to levofloxacin. Almost all S. aureus strains (99.2%) were resistant to penicillin, whereas only 2.4% were resistant to rifampicin. Of the 698 bacteria isolated, the prevalence of multidrug-resistant bacteria (MDR) was 41.3% (n = 288), whereas the prevalence of ESBL-producing Enterobacteriaceae was 51.3% (201/392). Conclusion: We reported various resistance profiles of bacterial species isolated from hospitalized patients at the CHU Sylvanus Olympio, Lomé, Togo. The study of bacterial ecology and resistance in every prefectural, regional, and university teaching hospital would be of great importance to reduce mortality associated with hospital-acquired infections throughout the Togolese territory.

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Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Profiles of Bacteria Isolated from Patients Hospitalized at the Sylvanus Olympio University Teaching Hospital in Lomé, Togo

Dossim,

Bawe,

Dossouvi

et al. 2024

MRJI

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Carbapenem resistance in West Africa: a systematic review

Dossouvi,

Bakpatina-Batako

2024

Microbiology Independent Research Journal (MIR Journal)

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OBJECTIVES: The World Health Organization (WHO) has reported carbapenem-resistant Enterobacteriaceae (CRE), carbapenem-resistant Acinetobacter baumannii (CRAb), and carbapenem-resistant Pseudomonas aeruginosa (CRPa) as critical priority pathogens for human health. Therefore, this study aimed to review clinical carbapenem resistance systematically and comprehensively in West Africa. DATA SOURCES: A total of 102 research articles on carbapenem resistance from the sixteen countries forming the West African region were included in this review. DATA SYNTHESIS: Carbapenem-resistant bacteria (CRB) were isolated mainly from urine 73/300 (24.3%) and pus/wounds of patients 69/300 (23%). The mean prevalence of CRB in West Africa was 4.6% (1902/41635), ranging from 1.6% to 18.6%. CRB identified were mainly Escherichia spp. (34/130; 26.1%), Klebsiella spp. (27/130, 20.8%), Pseudomonas spp. (26/130, 20%), and Acinetobacter spp. (25/130; 19.2%). Bacteria isolated in West African countries produced carbapenemases that belong to the four Ambler classes and include 13 types. The bla OXA-type (34/104; 32.7%), bla NDM (31/104; 29.8%), and bla VIM (13/104; 12.5%) were the most common carbapenemase genes. These genes are carried by plasmids, composite transposons, and integrons. The Kirby-Bauer disc diffusion method (74/172; 43.0%), PCR (38/172; 22.1%), and whole genome sequencing (17/172; 9.9%) were the most common methods for carbapenem resistance detection. The most reported alternative antibiotics active against CRB were amikacin, colistin, and fosfomycin. CONCLUSION: There is an urgent need to take synergistic action to delay, as much as possible, the occurrence of CRB epidemics in West Africa.

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Molecular Characterization of Clinical Strains of Extended-Spectrum Βeta-Lactamases-Producing Klebsiella Pneumoniae Isolated in A Tertiary Hospital in Dakar-Senegal

Cited by 2 publications

References 0 publications

Antibiotic Resistance Profiles of Bacteria Isolated from Patients Hospitalized at the Sylvanus Olympio University Teaching Hospital in Lomé, Togo

Antibiotic Resistance Profiles of Bacteria Isolated from Patients Hospitalized at the Sylvanus Olympio University Teaching Hospital in Lomé, Togo

Carbapenem resistance in West Africa: a systematic review

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