Molecular characterization of Cotton leaf curl Multan virus and DNA-satellites complex associated enation leaf curl and yellow vein mosaic disease of hollyhock

Abstract

Hollyhock is one important decorative plant grown in garden beds in different region of the the world. The ornamental plant is susceptible to many diseases caused by diverse pathogens. Among these viral pathogens can cause enormous damage to the ornamental plant. The aim of the present study was identification of begomovirus and DNA sateelites is associated with the yellow vein mosaic and enation leaf curl disease complex of hollyhock. The hollyhock plants showing the typical begomovirus-like symptoms were collected from Pusa campus, New Delhi (India). To know the status of the begomovirus, the total DNA isolated from the infected hollyhock was subjected to PCR amplification using primers specific to the begomovirus. The partial (1.2 kb) genome sequencing of ten hollyhock samples indicates the associated of begomovirus (nucleotide identities is more 95% among themselves). Therefore three representative samples (H1, H2, H3) full-length genome (DNA-A, betasatellite and alphasatellite) was amplified through RCA method. The pairwise comparision of complete genome of the begomoviruses, betasatellites and alphasatellites using Sequence Demarcation Tool (SDT) showed highest nucleotide (nt) identity of 88.0 to 92.7% (DNA-A) with Cotton leaf curl Multan virus, 92.5–96.7% with Ludwigia leaf distortion betasatellite and 90.4 to 93. 2% % with Ageratum enation alphasatellite. Further recombinantion analysis showed that the begomoviruses and DNA satellites under study was recombinants of previously reported begomoviruses and DNA sattelites. This is the first report of Cotton leaf curl Multan virus and DNA satellites associated complex disease of hollyhock in India.