Molecular characterization of CRBR2 fragment of TLR4 gene in association with mastitis in Vrindavani cattle

Panigrahi, Manjit; Kumar, Harshit; Nayak, Sonali Sonejita; Rajawat, Divya; Parida, Subhashree; Bhushan, Bharat; Sharma, Arjava; Dutt, Triveni

doi:10.1016/j.micpath.2022.105483

Cited by 10 publications

(2 citation statements)

References 33 publications

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“…The TLR4 consists of the composed of 16 to 28 leucine-rich repeats extracellular ligand-binding domain and the intracellular toll-interleukin I receptor domain (TIR). The cattle TLR4 gene located on chromosome 8 contains three exons and encodes 841 amino acids 14,15 . The TIR domain of TLR4 protein is 143 amino acids (between 673 and 816) long.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Computational Investigation of Polymorphisms in the TIR Domain of the TLR4 Protein in Holsteins Raised in Turkey

Atalay

2023

Journal of Research in Veterinary Medicine

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Increasing environmental temperatures as a result of climate change adversely affect livestock welfare and health. Moreover, temperatures increase the distribution and survival of parasites and infectious agents. Livestock diseases that cause significant economic losses are a worldwide concern. Toll like receptor 4 (TLR4) is an ideal marker gene candidate, due to the key role it plays in initiating the immune response against pathogens. In this study, the toll-interleukin-1 receptor (TIR) domain polymorphisms were investigated in the Turkish Holstein cattle by DNA sequencing. The effects of polymorphisms on the protein structure and function were evaluated by computational tools (I-Mutant Suite, Project Hope and PyMOL). The rs8193069 C>T polymorphism was detected in the TIR domain. The SNP causes threonine to isoleucine substitution at position 674 of the TLR4 protein which is the second amino acid of the TIR domain. The I-Mutant Suite predicted that Thr674Ile substitution can result in decreased protein stability (DDG= -0.40 Kcal/mol). Project Hope results showed that mutant and wild-type amino acids have different properties and that this can disturb the TIR domain. The Thr674Ile polymorphism observed in the TIR domain of TLR4 protein in Turkish Holstein cattle might affect the function of the TLR4 by causing physicochemical changes.

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Section: Introductionmentioning

confidence: 99%

Characterization and Computational Investigation of Polymorphisms in the TIR Domain of the TLR4 Protein in Holsteins Raised in Turkey

Atalay

2023

Journal of Research in Veterinary Medicine

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“…These native cattle breeds are particularly well suited to various agroclimatic conditions of India, and some of these kinds of cattle are also doing very well in other places across the world. Additionally, indigenous cattle breeds are essential due to their advantages in terms of disease resistance, low input management, and better survival in adverse weather conditions and climate change (Savalia et al, 2019;Panigrahi et al, 2022a). One of the key challenges in studying the signature of selection underlying climate adaptation is distinguishing between genetic changes that are driven by natural selection and those that result from other factors such as genetic drift or gene flow (Kumar et al, 2022).…”

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confidence: 99%

Deciphering climate resilience in Indian cattle breeds by selection signature analyses

Nayak,

Panigrahi,

Rajawat

et al. 2024

Trop Anim Health Prod

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The signature of selection is a crucial concept in evolutionary biology that refers to the pattern of genetic variation which arises in a population due to natural selection. In the context of climate adaptation, the signature of selection can reveal the genetic basis of adaptive traits that enable organisms to survive and thrive in changing environmental conditions. Breeds cultivated in diverse agroecological zones exhibit genetic "footprints" within their genomes that mirror the influence of climate-induced selective pressures, subsequently impacting phenotypic variance. We examined genotypic data from eight different cattle breeds (Gir, Hariana, Kankrej, Nelore, Ongole, Red Sindhi, Sahiwal, and Tharparkar) that are adapted to diverse regional climates in India. It is assumed that the genomes of animals residing in these regions have been altered through selection for various climatic adaptations. In this study, we utilized bovine 50K chip data and four genome scan techniques to assess the genetic regions of positive selection for high-temperature adaptation. We have also performed genome-wide investigation of genetic diversity, inbreeding, and effective population size in our target dataset. To identify selection signature regions in this work, we used four intra-population statistics: Tajima's D, CLR, iHS, and ROH. We identified potential regions for selection that is likely to be caused by adverse climatic conditions. We observed many adaption genes as several potential selection signature areas. These include genes like HSPB2, HSPB3, HSP20, HSP90AB1, HSF4, HSPA1B, CLPB, GAP43, MITF, and MCHR1, which have been reported in the cattle population that lives in varied climatic regions.

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Comprehensive selection signature analyses in dairy cattle exploiting purebred and crossbred genomic data

Nayak,

Panigrahi,

Rajawat

et al. 2023

Mamm Genome

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Molecular characterization of CRBR2 fragment of TLR4 gene in association with mastitis in Vrindavani cattle

Cited by 10 publications

References 33 publications

Characterization and Computational Investigation of Polymorphisms in the TIR Domain of the TLR4 Protein in Holsteins Raised in Turkey

Characterization and Computational Investigation of Polymorphisms in the TIR Domain of the TLR4 Protein in Holsteins Raised in Turkey

Deciphering climate resilience in Indian cattle breeds by selection signature analyses

Comprehensive selection signature analyses in dairy cattle exploiting purebred and crossbred genomic data

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