Molecular characterization of Cryptosporidium spp. from wild rats and mice from rural communities in the Philippines

Abstract

Accepted ManuscriptThis is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

Zoonotic Cryptosporidium spp. and Enterocytozoon bieneusi in pet chinchillas ( Chinchilla lanigera ) in China

“…hominis and C. parvum are the most common zoonotic Cryptosporidium species, and have been found in humans worldwide [4]. Rodents are naturally infected with several Cryptosporidium species or genotypes [9,10]. C. ubiquitum has been commonly detected in rodents including squirrels, chipmunks, woodchucks, beavers, porcupines, deer mice, house mice, and gerbils [18].…”

“…Rodents are naturally infected with several Cryptosporidium species or genotypes, and have been implicated as potential reservoirs of Cryptosporidium [7][8][9][10]. The genetic identity of E. bieneusi has been thoroughly investigated in humans and several domestic animals, but few studies have addressed the importance of this infection in rodents.…”

Specific and quantitative detection and identification of Cryptosporidium hominis andC. parvum in clinical and environmental samples

“…All DNA was extracted using a Powersoil DNA extraction kit (MOBIO, Carlsbad, California, USA), with minor modifications to the manufacturer's protocol as described by Ng-Hublin et al, (2013). The 10,000 oocyst/µl DNA stock was then serially diluted to create oocyst DNA concentrations equivalent to 1000, 100, 10, 1 oocysts/µl, respectively to be used for sensitivity testing and standard curve generation using Rotor-Gene 6.0.14 software.…”

Molecular Characterization of Cryptosporidium Species Isolated from Cattle in Southwest of Iran

“…Unfortunately, this method has a low sensitivity and is also not appropriate for determination of different species of Cryptosporidium (8). Molecular based techniques such as polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) have become popular worldwide (12, 13) due to high sensitivity and specificity in differentiate between Cryptosporidium species (7,(14)(15)(16)(17).…”