Molecular characterization of Cuban endemism Carica cubensis Solms using random amplified polymorphic DNA (RAPD) markers

Abstract

The objective of this work is to present an appropriate set of RAPD (random amplified polymorphic DNA) markers using single and multiplex PCR analysis suitable for the characterization of the endemic Cuban species Carica cubensis and the establishment of genetic relationships with the cultivated species Carica papaya. RAPD markers presented a high level of polymorphism. In addition, the incorporation of more than one RAPD primer in the PCR analysis increased the number of obtained bands and the polymorphism of these bands. A total of 73 RAPD bands were detected (45 of them polymorphic) with the nine RAPD markers assayed using single and multiplex PCR analysis. Results demonstrated a reduced genetic variability within the tested Carica cubensis accessions. The observed clustering in this species could be better explained according to geographic proximity and can indicate the similar precedence of the isolated studied populations. C. cubensis seem to be subspecies of C. papaya adapted to the environmental conditions of the mountains of Cuba or a endemic species close to C. papaya. The implications of these results in the creation of effective germplasm core collection in Carica species have been also discussed

Genetic variability and relationship studies in new Indian papaya (Carica papaya L.) germplasm using morphological and molecular markers

“…Among the molecular markers, RAPD and ISSR markers have been extensively used to study genetic diversity and relationships in papaya germplasm (Rodríguez et al, 2010;Sudha et al, 2013). Among these molecular markers, ISSRs are considered useful and have been extensively used for the identification of species or germplasms in a wide range of plants (Ahmad et al, 2010).…”

“…Several molecular markers, including random amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP), have been used for analysis of genetic diversity, relationships, and germplasm identification of papaya (de Jesus et al, 2013). Among the molecular markers, RAPD and ISSR markers have been extensively used to study genetic diversity and relationships in papaya germplasm (Rodríguez et al, 2010;Sudha et al, 2013). Among these molecular markers, ISSRs are considered useful and have been extensively used for the identification of species or germplasms in a wide range of plants (Ahmad et al, 2010).…”

Variabilidad morfoagronómica en especímenes de papayo silvestre (Carica cubensis Solms) en Cuba

“…La ubicación geográfica y aislamiento de la zona prospectada, el tipo de reproducción, los resultados de la caracterización molecular (Rodríguez et al, 2010) y la caracterización morfoagronómica en condiciones tradicionales de cultivo, indican que el papayo silvestre identificado constituye una población.…”

Use of morpho-agronomic traits and DNA profiling for classification of genetic diversity in papaya

“…The polymorphisms obtained from the analysis of these genotypes of C. papaya were more numerous than the 186 polymorphisms found by Kim et al (2002), when analyzing 63 papaya accessions with this same type of molecular marker. Moreover, Rodríguez et al (2010) reported only 45 polymorphic bands using nine RAPD primers when assessing the genetic diversity of the species Vasconcellea cubensis and C. papaya. Table 3.…”

“…Due to their many advantages, especially high polymorphism, reproducibility and wide genomic coverage in many species, amplified fragment length polymorphism (AFLP) markers have been used to characterize molecular diversity and to establish fingerprinting in various species (Kim et al, 2002, Rodríguez et al, 2010. Inter-simple sequence repeat (ISSR) markers constitute another PCR-based technique useful for genome studies.…”