Molecular characterization of duck interleukin-17

Molecular cloning, characterization and mRNA expression of duck interleukin-17F

“…Moreover, in a phylogenetic tree, duIL-17F formed a branch with chIL-17F in the same cluster as avian IL-17A (Fig. 1B) (Min and Lillehoj, 2002;Yoo et al, 2009;Wei et al, 2014b). The exon/intron organization of duIL-17F was quite similar to the chicken, mammalian, and piscine homologues (Fig.…”

“…This protein has a predicted molecular weight of 18.6 kDa (non-glycosylated) and a calculated isoelectric point of 9.81. Amino acid comparisons showed that duIL-17F shares 53.5% amino acid sequence identity with duIL-17A (Yoo et al, 2009), 77.5% with the chIL-17F (Kim et al, 2012) and 37-46% with corresponding mammalian homologues. Moreover, in a phylogenetic tree, duIL-17F formed a branch with chIL-17F in the same cluster as avian IL-17A (Fig.…”

“…The recent dramatic growth of the duck industry is anticipated to increase the occurrence of diseases including Salmonella infection (Cha et al, 2013). Recently, duIL-17A was identified in ConA-activated splenic lymphocytes (Yoo et al, 2009) leading us to become interested in identify and characterize the duIL-17F. In this study, we identified a full-length duIL-17F cDNA.…”

Comparative Immunology of Agricultural Birds

“…Chicken IL-17 was initially identified from an EST from Eimeriainfected intra-epithelial leukocytes (IEL) of chickens [129]. Primers based on the chicken sequence allowed identification of a duck IL-17 cDNA, which showed 84% similarilty to chicken IL-17 [130]. Transcripts for duIL-17 were detected in ConA-activated splenic lymphocytes.…”

“…Primers based on the chicken sequence allowed identification of a duck IL-17 cDNA, which showed 84% similarilty to chicken IL-17 [130]. Two chicken mAbs (IG8 and 2A2) [131] cross-reacted with epitopes from recombinant duck IL-17 in ELISA and on Western blots; they may prove useful as tools for characterization of the Th17 subpopulation in ducks [130]. Two chicken mAbs (IG8 and 2A2) [131] cross-reacted with epitopes from recombinant duck IL-17 in ELISA and on Western blots; they may prove useful as tools for characterization of the Th17 subpopulation in ducks [130].…”