Molecular characterization of enterohemorrhagic Escherichia coli hemolysin gene (EHEC- hlyA )-harboring isolates from cattle reveals a diverse origin and hybrid diarrheagenic strains

Badouei, Mahdi Askari; Morabito, Stefano; Najafifar, Arash; Mazandarani, Emad

doi:10.1016/j.meegid.2016.02.002

Cited by 17 publications

(12 citation statements)

References 37 publications

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“…This suggests that repeat sampling of individual animals within a closely related herd with a low turnover rate may contribute to decreased serotype diversity, particularly as the rumen microbiota stabilizes as the animal matures ( Jami et al, 2013 ). The predominance of phylotype B1 in this study is consistent with findings that suggest it is prevalent in ruminants ( Carlos et al, 2010 ), particularly among cattle isolates ( Coura et al, 2015 ; Askari Badouei et al, 2016 ).…”

Section: Discussionsupporting

confidence: 91%

“…Notably, serotypes with known pathogenic potential and encoding eae , shared a variant that clustered distinctly from other serotype-specific variants. This supports previous findings where RFLP patterns for the EHEC-haemolysin gene were generally serotype-specific and also delineated by the presence/absence of eae ( Boerlin et al, 1998 ; Askari Badouei et al, 2016 ). The identification of a number of other virulence-associated genes with roles in adherence, colonization, invasion, iron uptake and toxin production in the majority of strains suggests the potential to cause human disease exists amongst many of these commensal cattle isolates.…”

Section: Discussionsupporting

confidence: 91%

“…Indeed, VTEC/ETEC hybrids have been previously detected in humans and cattle ( Nyholm et al, 2015 ), and perhaps the most well-known example of an emerging pathogenic hybrid is the O104:H4 European outbreak of 2011, where an enteroaggregative E. coli O104:H4 strain acquired the Stx -2 bacteriophage and caused over 4,000 illnesses, and 50 deaths ( Grad et al, 2012 ). Cattle EHEC- hlyA encoding strains, which constituted the majority of strains in our study, may be a potential source of DEC strains of intermediate pathotypes ( Askari Badouei et al, 2016 ).…”

Section: Discussionmentioning

confidence: 97%

“…In this study, 88.7% of VTEC carried the EHEC haemolysin gene (EHEC- hlyA ), which is also known to be highly prevalent in human clinical VTEC ( Beutin et al, 2004 ). A survey of cattle herds in Iran detected EHEC- hlyA in 11.9% ( n = 452) of VTEC isolates ( Askari Badouei et al, 2016 ) and in a longitudinal study of closed cattle herds in Germany, the prevalence of EHEC- hlyA was 26.5% ( n = 1,647) ( Geue et al, 2002 ). In our study, genetic diversity was also observed within the EHEC- hlyA gene itself.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Multi-Year Persistence of Verotoxigenic Escherichia coli (VTEC) in a Closed Canadian Beef Herd: A Cohort Study

Wang

Jokinen²,

Laing

et al. 2018

Front. Microbiol.

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In this study, fecal samples were collected from a closed beef herd in Alberta, Canada from 2012 to 2015. To limit serotype bias, which was observed in enrichment broth cultures, Verotoxigenic Escherichia coli (VTEC) were isolated directly from samples using a hydrophobic grid-membrane filter verotoxin immunoblot assay. Overall VTEC isolation rates were similar for three different cohorts of yearling heifers on both an annual (68.5 to 71.8%) and seasonal basis (67.3 to 76.0%). Across all three cohorts, O139:H19 (37.1% of VTEC-positive samples), O22:H8 (15.8%) and O?(O108):H8 (15.4%) were among the most prevalent serotypes. However, isolation rates for serotypes O139:H19, O130:H38, O6:H34, O91:H21, and O113:H21 differed significantly between cohort-years, as did isolation rates for some serotypes within a single heifer cohort. There was a high level of VTEC serotype diversity with an average of 4.3 serotypes isolated per heifer and 65.8% of the heifers classified as “persistent shedders” of VTEC based on the criteria of >50% of samples positive and ≥4 consecutive samples positive. Only 26.8% (90/336) of the VTEC isolates from yearling heifers belonged to the human disease-associated seropathotypes A (O157:H7), B (O26:H11, O111:NM), and C (O22:H8, O91:H21, O113:H21, O137:H41, O2:H6). Conversely, seropathotypes B (O26:NM, O111:NM) and C (O91:H21, O2:H29) strains were dominant (76.0%, 19/25) among VTEC isolates from month-old calves from this herd. Among VTEC from heifers, carriage rates of vt1, vt2, vt1+vt2, eae, and hlyA were 10.7, 20.8, 68.5, 3.9, and 88.7%, respectively. The adhesin gene saa was present in 82.7% of heifer strains but absent from all of 13 eae+ve strains (from serotypes/intimin types O157:H7/γ1, O26:H11/β1, O111:NM/θ, O84:H2/ζ, and O182:H25/ζ). Phylogenetic relationships inferred from wgMLST and pan genome-derived core SNP analysis showed that strains clustered by phylotype and serotype. Further, VTEC strains of the same serotype usually shared the same suite of antibiotic resistance and virulence genes, suggesting the circulation of dominant clones within this distinct herd. This study provides insight into the diverse and dynamic nature of VTEC populations within groups of cattle and points to a broad spectrum of human health risks associated with these E. coli strains.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Multi-Year Persistence of Verotoxigenic Escherichia coli (VTEC) in a Closed Canadian Beef Herd: A Cohort Study

Wang

Jokinen²,

Laing

et al. 2018

Front. Microbiol.

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“…The virulence of this strain was assessed in vitro , showing that it induced A/E lesions and produced a functional heat-label toxin (LT) (Dutta et al, 2015 ). In 2016, a new type of EPEC/ETEC strain bearing the heat-stable toxin (ST) instead of LT-encoding genes was reported in healthy cattle (Askari Badouei et al, 2016 ), but the ST expression was not evaluated. Hazen et al ( 2017 ) reported the identification of four EPEC/ETEC hetero-pathogenic strains isolated from children, two of them being with diarrhea, one asymptomatic, and another with lethal outcome.…”

Section: Combinations Of Virulence Factors That Lead To More Severe Dmentioning

confidence: 99%

Diversity of Hybrid- and Hetero-Pathogenic Escherichia coli and Their Potential Implication in More Severe Diseases

Santos¹,

Santos²,

Silva³

et al. 2020

Front. Cell. Infect. Microbiol.

112

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Although extraintestinal pathogenic Escherichia coli (ExPEC) are designated by their isolation site and grouped based on the type of host and the disease they cause, most diarrheagenic E. coli (DEC) are subdivided into several pathotypes based on the presence of specific virulence traits directly related to disease development. This scenario of a well-categorized E. coli collapsed after the German outbreak of 2011, caused by one strain bearing the virulence factors of two different DEC pathotypes (enteroaggregative E. coli and Shiga toxin-producing E. coli ). Since the outbreak, many studies have shown that this phenomenon is more frequent than previously realized. Therefore, the terms hybrid- and hetero-pathogenic E. coli have been coined to describe new combinations of virulence factors among the classic E. coli pathotypes. In this review, we provide an overview of these classifications and highlight the E. coli genomic plasticity that results in some mixed E. coli pathotypes displaying novel pathogenic strategies, which lead to a new symptomatology related to E. coli diseases. In addition, as the capacity for genome interrogation has grown in the last few years, it is clear that genes encoding some virulence factors, such as Shiga toxin, are found among different E. coli pathotypes to which they have not traditionally been associated, perhaps foreshowing their emergence in new and severe outbreaks caused by such hybrid strains. Therefore, further studies regarding hetero-pathogenic and hybrid-pathogenic E. coli isolates are necessary to better understand and control the spread of these pathogens.

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Antibiotic resistance profiling and phylotyping of human-diarrheagenic Escherichia coli pathotypes detected from diarrheic and non-diarrheic calves in Iran

Naderi,

Ghanbarpour,

Jajarmi

et al. 2024

Mol Biol Rep

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Molecular characterization of enterohemorrhagic Escherichia coli hemolysin gene (EHEC- hlyA )-harboring isolates from cattle reveals a diverse origin and hybrid diarrheagenic strains

Cited by 17 publications

References 37 publications

Multi-Year Persistence of Verotoxigenic Escherichia coli (VTEC) in a Closed Canadian Beef Herd: A Cohort Study

Multi-Year Persistence of Verotoxigenic Escherichia coli (VTEC) in a Closed Canadian Beef Herd: A Cohort Study

Diversity of Hybrid- and Hetero-Pathogenic Escherichia coli and Their Potential Implication in More Severe Diseases

Antibiotic resistance profiling and phylotyping of human-diarrheagenic Escherichia coli pathotypes detected from diarrheic and non-diarrheic calves in Iran

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