Molecular characterization of enteroviruses detected in Gyeong-Ju and Po-Hang provinces of Korea in 2003

Jeong, Eunju; Lee, Jung‐Hee; Kim, Mi-Sun; Bae, Geun-Ryang; Jung, Cheoll; Lee, Kwan; Choi, Sung-Min; Kim, Doo Kwun; Lee, Dong‐Seok; Kim, Won-Duck; Jee, Youngmee; Cheong, Hae-Kwan; Lee, Seung–Hyun

doi:10.1007/s00705-010-0755-4

Cited by 21 publications

(10 citation statements)

References 13 publications

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“…In previous Korean studies 21 , 22 , 23 , 24 ), outbreaks of aseptic meningitis caused by various serotypes of EV such as Echo 6, Echo 9, Echo11, Echo13, Echo 18, Echo 25, Echo30, and EV 71 were reported. In this study, Echo 30 (9 patients) was the most frequently detected serotype, which has been previously associated with an epidemic in Gyeongbuk province in 2003 23 ). Some of the limitations of this study are that the study was retrospective, included one center study and used sample and subject information that was previously obtained by the Bank.…”

Section: Discussionmentioning

confidence: 94%

Prevalence of human parechovirus and enterovirus in cerebrospinal fluid samples in children in Jinju, Korea

et al. 2015

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PurposeHuman parechovirus (HPeV) and enterovirus (EV) are causative agents of a sepsis-like illness in neonates and of infections of the central nervous system in young children. The objectives of this study were to assess the prevalence of HPeV3 and EV infection in young children with a sepsis-like illness or with meningitis in Jinju, Korea.MethodsCerebrospinal fluid (CSF) samples were collected from 267 patients (age range, 1 day to 5 years) and assessed for HPeV and EV by performing reverse transcription polymerase chain reaction assay. Amplification products of the VP3/VP1 region of HPeV and of the VP1 region of EV were sequenced to identify the virus type.ResultsHPeV and EV were detected in 3.4% and 7.5% of the total CSF samples assessed, respectively. The age distribution of EV-positive patients (median age, 1.4 months) had a significantly broader range than that of HPeV-positive patients (median age, 7.8 months). The peak seasons for HPeV and EV infection were spring and summer, respectively. The clinical symptoms for HPeV and EV infection were similar, and fever was the most common symptom. Pleocytosis was detected in 22.2% of HPeV-positive patients and 35.5% of EV-positive patients. The VP3/VP1 gene sequence of the nine Korean strains clustered most closely with the Japanese strain (AB759202).ConclusionThe data indicate that HPeV infection is predominant in young infants (<6 months) and that meningitis without pleocytosis was caused by both HPeV and EV infection in children.

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Section: Discussionmentioning

confidence: 94%

Prevalence of human parechovirus and enterovirus in cerebrospinal fluid samples in children in Jinju, Korea

et al. 2015

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“…The earliest characterized subgenotype C4 strain SHZH98 is in cluster b, but for more systematic classification (usually based on chronological order), the older and recent strains should belong to cluster a and b respectively. Since the late 1990s, EV71 subgenotype C4 strains have been frequently reported in Asian countries [22,[32][33][34][35][36][37]. The subgenotype C4 was further divided into two clusters, C4b and C4a [22,37], which are better to be renamed as genotypes D1a and D1b respectively.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of EV71 isolates from 2004 to 2010 reveals predominance and persistent circulation of the newly proposed genotype D and recent emergence of a distinct lineage of subgenotype C2 in Hong Kong

Yip

Lau

et al. 2013

Virol J

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BackgroundEnterovirus 71 (EV71) is a common etiological agent of hand, foot and mouth disease (HFMD) in children. EV71 epidemics have been reported in Hong Kong in recent years, and yet the genetic information of EV71 strains circulating in our locality is limited. The objective of this study was to investigate the genetic evolution of these EV71 isolates in Hong Kong over a 7-year period.MethodsTwenty-two EV71 isolates from Hong Kong during 2004–2010 were included for phylogenetic analysis using partial VP2-VP3, 2C and 3D regions. Eight EV71 strains were selected for complete genome sequencing and recombination analysis.ResultsAmong the 22 EV71 isolates, 20 belonged to subgenotype C4 and 2 belonged to subgenotype C2 based on the phylogenetic analysis of partial VP2-VP3, 2C and 3D gene regions. Phylogenetic, similarity plot and bootscan analyses using complete genome sequences of seven EV71 isolates of subgenotype C4 supported that the “double-recombinant” strains of subgenotype C4 persistently circulating in Hong Kong should belong to a newly proposed genotype D. Further analysis revealed two clusters, subgenotypes C4b and C4a (proposed genotypes D1a and D1b respectively), with “genotype D1b” strains being predominant in recent years in Hong Kong. A distinct lineage of EV71 subgenotype C2 has emerged in Hong Kong in 2008. The evolutionary rate of EV71 was 3.1 × 10-3 nucleotide substitutions per site per year similar to that of other enterovirus, such as EV68, but was relatively lower than those of echovirus 30 and poliovirus. Molecular clock analysis using VP1 gene dated the time to the most recent common ancestor of all EV71 genotypes to 1900s, while the EV71 “double-recombinant” strains of “genotype D” were detected as early as 1998.ConclusionsThis study provides the molecular basis for proposing a new “genotype D” of EV71 and assigning a discrete lineage of subgenotype C2. EV71 strains of “genotype D” have been circulating in Hong Kong for over 7 years, with “genotype D1b” being predominant.

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“…44 In 1997, a large outbreak of EV71 in Sarawak, Malaysia, heralded the start of a new series of outbreaks across the Asia-Pacifi c region (table 2). [9][10][11][12][13][14][45][46][47][48][49][50][51][52][53] In Sarawak, 2618 HFMD cases and 34 deaths were recorded between May and July, 1997; around the same time EV71 caused four deaths in peninsular Malaysia and several cases of severe neurological disease in Japan. 4,11,54 In 1998, the largest EV71 epidemic so far occurred in Taiwan: 7 an estimated 1·5 million people were infected and 405 children were admitted to hospital for serious neurological complications, of whom 78 died.…”

Section: Asia-pacifi C Regionmentioning

confidence: 99%

Virology, epidemiology, pathogenesis, and control of enterovirus 71

Solomon

Lewthwaite

Perera

et al. 2010

The Lancet Infectious Diseases

1,153

977

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First isolated in California, USA, in 1969, enterovirus 71 (EV71) is a major public health issue across the Asia-Pacifi c region and beyond. The virus, which is closely related to polioviruses, mostly aff ects children and causes hand, foot, and mouth disease with neurological and systemic complications. Specifi c receptors for this virus are found on white blood cells, cells in the respiratory and gastrointestinal tract, and dendritic cells. Being an RNA virus, EV71 lacks a proofreading mechanism and is evolving rapidly, with new outbreaks occurring across Asia in regular cycles, and virus gene subgroups seem to diff er in clinical epidemiological properties. The pathogenesis of the severe cardiopulmonary manifestations and the relative contributions of neurogenic pulmonary oedema, cardiac dysfunction, increased vascular permeability, and cytokine storm are controversial. Public health interventions to control outbreaks involve social distancing measures, but their eff ectiveness has not been fully assessed. Vaccines being developed include inactivated whole-virus, live attenuated, subviral particle, and DNA vaccines.

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Molecular characterization of enteroviruses detected in Gyeong-Ju and Po-Hang provinces of Korea in 2003

Cited by 21 publications

References 13 publications

Prevalence of human parechovirus and enterovirus in cerebrospinal fluid samples in children in Jinju, Korea

Prevalence of human parechovirus and enterovirus in cerebrospinal fluid samples in children in Jinju, Korea

Genetic characterization of EV71 isolates from 2004 to 2010 reveals predominance and persistent circulation of the newly proposed genotype D and recent emergence of a distinct lineage of subgenotype C2 in Hong Kong

Virology, epidemiology, pathogenesis, and control of enterovirus 71

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