Molecular characterization of equine infectious anaemia virus from a major outbreak in southeastern France

Gaudaire, Delphine; Lecouturier, Fanny; Ponçon, Nicolas; Morilland, E.; Laugier, Claire; Zientara, Stéphan; Hans, Aymeric

doi:10.1111/tbed.12657

Cited by 19 publications

(17 citation statements)

References 22 publications

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“…Phylogenetic analysis of 22 Slovenian sequences covering 222 nucleotides of the 5' LTR region and the gag gene, showed clustering together and the separation of Chinese and American sequences [37]. Also, phylogenetic analysis of 50 French sequences covering about 1.400 nucleotides of the gag gene clustered separating Chinese, American and Asian sequences from other European region sequences [58].…”

Section: Discussionmentioning

confidence: 93%

Equine infectious anemia virus in naturally infected horses from the Brazilian Pantanal

et al. 2018

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Equine infectious anemia (EIA) has a worldwide distribution, and is widespread in Brazil. The Brazilian Pantanal presents with high prevalence comprising equine performance and indirectly the livestock industry, since the horses are used for cattle management. Although EIA is routinely diagnosed by the agar gel immunodiffusion test (AGID), this serological assay has some limitations, so PCR-based detection methods have the potential to overcome these limitations and act as complementary tests to those currently used. Considering the limited number of equine infectious anemia virus (EIAV) sequences which are available in public databases and the great genome variability, studies of EIAV detection and characterization molecular remain important. In this study we detected EIAV proviral DNA from 23 peripheral blood mononuclear cell (PBMCs) samples of naturally infected horses from Brazilian Pantanal using a semi-nested-PCR (sn-PCR). The serological profile of the animals was also evaluated by AGID and ELISA for gp90 and p26. Furthermore, the EIAV PCR amplified DNA was sequenced and phylogenetically analyzed. Here we describe the first EIAV sequences of the 5' LTR of the tat gene in naturally infected horses from Brazil, which presented with 91% similarity to EIAV reference sequences. The Brazilian EIAV sequences also presented variable nucleotide similarities among themselves, ranging from 93,5% to 100%. Phylogenetic analysis showed that Brazilian EIAV sequences grouped in a separate clade relative to other reference sequences. Thus this molecular detection and characterization may provide information about EIAV circulation in Brazilian territories and improve phylogenetic inferences.

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Section: Discussionmentioning

confidence: 93%

Equine infectious anemia virus in naturally infected horses from the Brazilian Pantanal

et al. 2018

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“…Penyakit ini disebabkan oleh virus EIA dan pertama kali ditemukan tahun 1840, kemudian dilaporkan di Asia seperti China (Wang et al 2018), Jepang (Dong et al 2013), Eropa seperti Perancis (Gaudaire et al 2018), Belgia (Caij & Tignon 2014), Brazil (Alice et al 2013), Amerika Serikat (Issel et al 2012), Argentina (Ricotti et al 2016) dan Irlandia (Quinlivan et al 2013). Lebih lanjut, penyebaran infeksi EIA di beberapa negara lainnya telah dilaporkan seperti tertuang pada Tabel 1.…”

Section: Situasi Penyakit Eia DI Duniaunclassified

Anticipation of the entry and spread of Equine Infectious Anemia in Horses in Indonesia

et al. 2019

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Horse is an attractive commodity in the world of horse matches, both for horse racing and equistrian. Recently, Asian countries including Indonesia have conducted international horse races. Horse traffic from several countries is unavoidable. This movement can lead to the entry of new diseases or the transmission of imported horses from local horses. At present, data on horse disease in Indonesia has not been widely reported, including Equine Infectious Anemia (EIA). This paper discusses EIA disease in horses about the etiology, characterization, spread of disease, its status in Indonesia and its treatment. EIA is a chronic disease characterized by high fever and thrombocytopenia. One of the factors that spread EIA infection, namely through traffic or horse movements. The highest risk factors for the spread of EIA disease are in the equestrian sector, followed by horse as a hobby, horse show or fattening horses for consumption. Wet environmental factors have higher risk to infection than dry areas. Applying the proper quarantine system, monitoring horse disease through surveillance, and implementing biosecurity at farms, location of events and at veterinary clinics, need to be done so that the entry of EIA disease can be anticipated as early as possible. This paper is expected to be useful and can be used as input for policy makers in the horse imports and movement.

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“…Equine infectious anemia virus (EIAV) is one of the most devastating and notifiable viral diseases of the family Equidae [1]. EIAV infection ranges from acute, subacute, and chronic infection.…”

Section: Introductionmentioning

confidence: 99%

“…EIAV belongs to the family Retroviridae and genus Lentivirus . EIAV was reported in many parts of the world, including North America [5], South America [6], Europe [1], and Asia [7-11]. Unfortunately, no medication that treats EIAV-infected horses is available.…”

Section: Introductionmentioning

confidence: 99%

Surveillance of the equine infectious anemia virus in Eastern and Central Saudi Arabia during 2014-2016

Alnaeem

Hemida

2019

Vet World

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Background: Equine infectious anemia virus (EIAV) is one of the most important threats to the equine industry globally. This is due to the poor performance of the affected horses, which requires euthanization of the infected animals upon the infection confirmation. Infected animals remain carriers throughout their life. EIAV infection has been reported in many parts of the world, including North America, Europe, Asia, and Africa. However, the EIAV status is never assessed in horses in the Gulf area, especially in the Kingdom of Saudi Arabia (KSA). Aim: This study aimed to perform molecular and serological surveillance among some horse populations in Eastern and Central Saudi Arabia. Materials and Methods: Sera and whole blood were collected from 361 horses and 19 donkeys from the eastern and central regions of Saudi Arabia during January 2014-December 2016. Sera were tested by the commercial EIAV enzyme-linked immunosorbent assay kits. Moreover, the collected blood samples were tested by the commercial real-time polymerase chain reaction kits. Results: Our serological surveillance revealed the absence of any antibodies against EIAV in the tested animals. Similar results were reported for the tested horses' plasma. This study confirms the absence of EIAV in horses and donkeys from Eastern and Central Saudi Arabia during the tenure of the current study. However, careful monitoring of the EIAV is highly recommended to avoid the emergence of such a virus in the horse population in Saudi Arabia. Conclusion: To the best of our knowledge, this is the first EIAV surveillance conducted not only in Saudi Arabia but also in the Gulf area. This study confirms the absence of EIAV in the tested equine population in the eastern and central regions of Saudi Arabia during 2014-2016.

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Molecular characterization of equine infectious anaemia virus from a major outbreak in southeastern France

Cited by 19 publications

References 22 publications

Equine infectious anemia virus in naturally infected horses from the Brazilian Pantanal

Equine infectious anemia virus in naturally infected horses from the Brazilian Pantanal

Anticipation of the entry and spread of Equine Infectious Anemia in Horses in Indonesia

Surveillance of the equine infectious anemia virus in Eastern and Central Saudi Arabia during 2014-2016

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