Molecular Characterization of ESBLs Genes among Multi-drug Resistant Klebsiella species in Ile-Ife South-West, Nigeria

Thonda, Oluwakemi Abike; Oluduro, A

doi:10.9734/jamb/2017/37925

Cited by 2 publications

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“…The reported distribution of Klebsiella pneumoniae in comparison to other Klebsiella spp in this study is closely in-line with that of Dallal et al, 13 who reported Klebsiella pneumoniae of (94%), and Klebsiella oxytoca (4%) in their study, but differs from that of Thonda and Oluduro 14 , who reported Klebsiella pneumoniae of (46%), Klebsiella oxytoca (19%), and K. ornithinolytica (6%). This observation may be due to differences in study population and sample source.…”

Section: Discussionsupporting

confidence: 88%

Prevalence of extended spectrum Beta-Lactamase producing Klebsiella species from patients’ specimens in a tertiary teaching hospital in Ile-Ife, Southwest Nigeria

Ajimuda¹,

Sanmi-Kayode²,

Adeniyi³

et al. 2022

Afr H. Sci.

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Objective: This study determined the prevalence of ESBL genes amongst Klebsiella species isolated from patients’ specimens attending a Tertiary Teaching Hospital, in Ile-Ife, Southwest Nigeria. Methods: A cross sectional study of presumptive isolates of Klebsiella (n=180) were collected, after ethical approval in the microbiology laboratory. Isolates were identified to species level by conventional biochemical tests and MicrobactTM 24E Identification Kits. Antibiotic susceptibility testing was performed by the Kirby Bauer’s disk diffusion method. ESBL production was detected by the double disc synergy and ESBL genes by the multiplex PCR protocol. Results: The Klebsiella species identified were Klebsiella pneumoniae 95.6%, Klebsiella oxytoca 3.3%, Klebsiella ornithinolytica 0.6% and Klebsiella terrigena 0.6%. The prevalence of ESBL genes among the Klebsiella isolates was 47.2% , and the most common ESBL gene was blaSHV (38.9%), also 75% of the study isolates had MAR index greater than 0.2. Conclusions: The study establishes the prevalence of Extended Spectrum Beta-Lactamase producing Klebsiella sp in the hospital and identified the most prevalent ESBL genes circulating as blaSHV followed by blaTEM and blaCTX-M in this environment. This underscores the need for regular and continuous surveillance of antimicrobial resistance trend as a strong component of the antibiotics stewardship and infection prevention and control programs in the hospital. Keywords: Antibiotic Resistance; Klebsiella; ESBL.

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Section: Discussionsupporting

confidence: 88%

Prevalence of extended spectrum Beta-Lactamase producing Klebsiella species from patients’ specimens in a tertiary teaching hospital in Ile-Ife, Southwest Nigeria

Ajimuda¹,

Sanmi-Kayode²,

Adeniyi³

et al. 2022

Afr H. Sci.

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“…Seventeen genes were detected in animals, 28 in humans, and 12 in the environment. These genes belong to the AmpC, 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 extended-spectrum, 10 , 11 , 12 , 13 , 14 , 15 , 17 , 19 , 20 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 narrow-spectrum, 13 , 14 , 15 , 16 , 19 , 24 , …”

Section: Resultsmentioning

confidence: 99%

Beta-lactamase resistance genes in Enterobacteriaceae from Nigeria

et al. 2022

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Background: Beta-lactamase genes are one of the most important groups of antimicrobial resistance genes in human and animal health. Therefore, continuous surveillance of this group of resistance genes is needed for a better understanding of the local epidemiology within a country and global dissemination.Aim: This review was carried out to identify different beta-lactamase resistance genes reported in published literature from Nigeria.Methods: Systematic review and meta-analysis was carried out on eligible Nigerian articles retrieved from electronic literature searches of PubMed ® , African Journals Online, and Google Scholar published between January 1990 and December 2019. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses method was adopted to facilitate clarity and transparency in reporting review findings.Results: Fifty-seven articles were included. All beta-lactamases reported were detected from Gram-negative bacteria, particularly from Enterobacteriaceae. Thirty-six different betalactamase genes were reported in Nigeria. These genes belong to the narrow-spectrum, AmpC, extended-spectrum and carbapenemase beta-lactamase resistance genes. The pooled proportion estimate of extended-spectrum beta-lactamase genes in Nigeria was 31% (95% confidence interval [CI]: 26% -36%, p < 0.0001), while the estimate of the bla CTX-M-15 gene in Nigeria was 46% (95% CI: 36% -57%, p < 0.0001). The proportion estimate of AmpC genes was 32% (95% CI: 11% -52%, p < 0.001), while the estimate for carbapenemases was 8% (95% CI: 5% -12%, p < 0.001). Conclusion:This study provides information on beta-lactamase distribution in Nigeria. This is necessary for a better understanding of molecular epidemiology of clinically important beta-lactamases, especially the extended-spectrum beta-lactamases and carbapenemases in Nigeria.

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Molecular Characterization of ESBLs Genes among Multi-drug Resistant Klebsiella species in Ile-Ife South-West, Nigeria

Cited by 2 publications

References 0 publications

Prevalence of extended spectrum Beta-Lactamase producing Klebsiella species from patients’ specimens in a tertiary teaching hospital in Ile-Ife, Southwest Nigeria

Prevalence of extended spectrum Beta-Lactamase producing Klebsiella species from patients’ specimens in a tertiary teaching hospital in Ile-Ife, Southwest Nigeria

Beta-lactamase resistance genes in Enterobacteriaceae from Nigeria

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