Molecular characterization of Escherichia coli isolates from patients with urinary tract infections in Palestine

Abstract

Antibiotic resistance of Escherichia coli isolated from urinary tract infections (UTIs) is increasing worldwide. A total of 41 E. coli isolates were obtained from urine samples from hospitalized patients with a UTI in three hospitals in the northern districts of the West Bank, Palestine during March and June 2011. Resistance rates were: erythromycin (95 %), trimethoprimsulfamethoxazole (59 %), ciprofloxacin (56 %), gentamicin (27 %), imipenem (22 %), amoxicillin (93 %), amoxicillin-clavulanic acid (32 %), ceftazidime (66 %) and cefotaxime (71 %). No meropenem-resistant isolates were identified in this study. Among the isolates, phylogenetic group B2 was observed in 13 isolates, D in 12 isolates, A in 11 isolates and B1 in five isolates. Thirty-five of the isolates were positive for an extended-spectrum b-lactamase phenotype. Among these isolates, the bla CTX-M gene was detected in 25, and eight harboured the bla TEM gene. None of the isolates contained the bla SHV gene. Transformation experiments indicated that some of the b-lactamase genes (i.e. bla CTX-M and bla TEM ) with co-resistance to erythromycin and gentamicin were plasmid encoded and transmissible. Apart from this, enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) revealed that the 41 isolates were genetically diverse and comprised a heterogeneous population with 11 ERIC-PCR profiles at a 60 % similarity level.

Detection of Metallo-?-Lactamasesencoding Genes Among Clinical Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia

“…In this present study, the maximum number (19 isolates) of urine carried 40% of MBLs producing gene which agreed with other study defined previously (Anago et al, 2015). Furthermore, a study in Indonesia in which sputum was the highest carriage of these genes (Karuniawati et al, 2013) (Adwan et al, 2014). In contrast, the higher resistant rate (82%) was observed in 2015 (Rezai et al, 2015) while 100% isolates were imipenem resistant (Liang et al, 2017) compared to this present study.…”

Phylogenetic analysis of multidrug resistant E. coli isolates from the urinary tract in Bushenyi district, Uganda using the new Clermont phylotyping method

“…Despite the fact that, some of the antimicrobial agents in the study demonstrated substantial sensitivities, some uropathogens showed extremely high level of AMR, more especially to erythromycin 74/86 (86.0%). The Gram negative bacteria demonstrated 56/56 (100.0%) resistance to erythromycin, this finding is more similar to previous reports by Kyabaggu et al (2007); Kibret and Abera, (2011) and Adwan et al (2014). The Gram positive bacteria resistance to erythromycin of 18/30 (60.0%) reported in this study is more comparable to previous report by Kabugo et al (2016).…”

Antimicrobial resistance among GLASS pathogens in conflict and non-conflict affected settings in the Middle East: A systematic review

“…From these tables we can observe that studies conducted in non-con ict affected countries appeared to be slightly more focused in terms of site of infection under investigation compared to studies conducted in con ict affected countries, where in a substantial proportion of cases the site of infection was not speci ed, or the authors were analysing resistance pro les of bacteria isolated from a miscellanea of specimens. Publication bias was assessed for a sub-sample of ten studies which presented the proportion of ESBL producers among E. coli isolates on urinary samples, six of which were conducted in con ict-affected countries (45,54,59,75,114,159) and four in non-con ict settings (154,155,164,165). Figure 4 shows the funnel plots elaborated on the logarithm of the proportion of ESBL producers amongst E. coli isolates in urine samples in all studies, and in studies conducted in con ict and non-con ict settings separately.…”