Molecular characterization of extended spectrum β-lactamase-producing Escherichia coli in a university hospital in Morocco, North Africa

Abstract

Introduction: ␤-Lactams are among the most widely prescribed antibiotics in human medicine. However, because of their massive and usually inappropriate use, resistance to these drugs has increased markedly, especially due to extended-spectrum ␤-lactamase (ESBL) production. Objectives: The aims of this study were to determine the prevalence of urinary Escherichia coli strains isolated from urine samples taken from patients diagnosed with urinary tract infections (UTIs), to evaluate their current antimicrobial susceptibility pattern and to look for blaSHV, blaTEM and blaCTX-M genes in these multi-drug resistant isolates. Subject and methods: A retrospective survey was made over 3 years from 2010 to 2012. It included all uropathogenic E. coli strains isolated from urine samples taken from consulting and hospitalized patients in the Avicenne Teaching Hospital in Marrakech, Morocco. Results: E. coli was the etiologic agent in 63% of reported UTIs due to Enterobacteriacae. In all, the prevalence of ESBL-producing E. coli reached 6% of all urinary Enterobacteriaceae isolates in 2012.

Prevalence of co-existence genes and clonal spread of ESBL-producing isolates causing hospital and community-acquired infections in Zenica-Doboj Canton, Bosnia and Herzegovina

“…Two (A and B) groups of Citrobacter freundii were identified by pulsed field gel electrophoresis typing using the 80% similarity (e). with the findings in Morocco, where E. coli was the most frequently noticed (23).…”

Extended spectrum beta‐lactamase producing Shiga‐toxin producing‐Escherichia coliin piglets, humans and water sources in North East region of India

“…Among the ESBLs genes, plasmid‐encoded temoneira (TEM), sulfhydryl variable (SHV) and cefotaximase (CTX‐M) are the most prevalent and clinically relevant. The distribution of ESBLs also varies according to the usage of antimicrobial agents in each geographical region, hospital set up or local community (El bouamri et al ; Ferjani et al ).…”

Antimicrobial susceptibility pattern of Klebsiella pneumoniae in the period 2013-2017

“…The high percentage of resistance to trimethoprim/ sulfamethoxazole is most likely due to a large use of this drug in the treatment of urinary infections. The resistance data confirm that this antibiotic should no longer be used as the first line therapy in uncomplicated urinary infections [24]. Over the past 20 years, a wide range of antimicrobial drugs has been used in the treatment of urinary tract infections caused by Gramnegative bacteria in the world.…”