Molecular Characterization of Fenugreek (Trigonella foenum-graecum L.) Genotypes Using Rapd Markers

Mamatha, N. C.; Tehlan, S. K.; Srikanth, M.; Shivaprasad, M.; Reddy, P. Karthik

doi:10.20546/ijcmas.2017.606.306

Cited by 6 publications

(1 citation statement)

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“…The polymorphism percentage and variation in the propagated plants' genetic stability could be explained for many reasons. Such reasons are explained by Sundaram and Purwar (2011) and Mamatha et al (2017) who applied RAPD-PCR to characterize the different fenugreek genotypes and accessions. Also, Hanafy and Akladious (2018) applied RAPD-PCR to estimate the effect of gamma radiation on fenugreek plant.…”

Section: Molecular Analysismentioning

confidence: 99%

Effects of ultrasonic waves on seedling growth, biochemical constituents, genetic stability of fenugreek (Trigonella foenum-graecum) under salinity stress

El-Sattar

Tawfik

2022

Vegetos

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Fenugreek is a globally important legume that is widely cultivated for its therapeutic benefits in most parts of the world. Seeds on the other hand have a poor germination and growth rate when exposed to salinity. The effect of ultrasonic exposure period on germination and early seedling behaviors of fenugreek seeds under salt stress was investigated in a laboratory experiment. During germination and early seedling stages, all tests were conducted at 40 kHz in a water bath ultrasonic device with two durations (10 and 20 min) under salinity stress using different concentrations of NaCl (0, 1000, 3000, and 5000 mg/l). The results revealed a substantial decrease in germination percentage, all growth criteria, with increasing NaCl concentration and a significant increase in biomass produced by the Fenugreek (total soluble protein, total soluble carbohydrate, and proline), all of which are thought to be mechanisms for salinity resistance. Ultrasonication of fenugreek seeds for 10 and 20 min has a significant impact on seed germination, early seedling development and biochemical constituents under normal and stress conditions. The genetic stability of fenugreek DNA content was affected by these different treatments. This variation was estimated by RAPD-PCR molecular marker, and resulted in a total polymorphism percentage of 49.72% from all the primers. All these different treatments caused variation in the physiological responses and DNA content. This variation enhanced with more ultrasonic and salt treatments. Hence, these stresses can be used for enhancing the variable metabolic processes in fenugreek plant and stimulate its medicinal properties.

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Section: Molecular Analysismentioning

confidence: 99%