Molecular characterization of fertile hydatid cysts from the liver of the sheep and cows and associated environmental influence factors

Abstract

The aim of the study is characterizing of hydatid cysts that have been isolated from sheep and cow liver fertile hydatid cysts using mitochondrial cytochrome c oxidase subunit 1(cox1) gene. DNA samples of nineteen sheep and one cow were extracted from protoscoleces and germinal layers of the parasites in Koya city-Erbil, Iraq, using specific extraction procedures. Mitochondrial cox1 gene region was amplified by polymerase chain reaction (PCR), and the expected gene sizes were confirmed by gel electrophoresis. All DNA isolates were then sequenced. Nucleotide sequence alignments were then performed to verify the sequenced isolated according to the database, which showed that all samples were belonging to the (G1) sheep strain. Phylogenetic analysis was also carried out for the sequenced isolated to find out the highest similarities with closest organisms to E. granulosus' conserved gene and to reveal sharing common ancestor, which has been confirmed. Electrocatalytic reduction of DNA where detected through applying cyclic-voltammetry technique, which referred to the environmentally strong protection features of these strains against any effects of external factors, such as heavy metals and has revealed the secret behind the potent preservation of the DNA structure of this parasite from being affect by mutations, or alterations, along the different lineages over a long period of time.

A study of the incidence of Lucilia sericata fly in ovine in Mosul city

“…The behavior of infested animal depends on the localization and the numbers of larvae (7). The incidence rate of Lucilia sericata is determined by several factors such as wool length, faecal soiling, sheep susceptibility and flies abundance, high rainfall and high temperature (8).…”

Nanoparticles as a New Approach for Treating Hydatid Cyst Disease

“…In this regard, and most recently, nucleotide sequence alignments of mitochondrial cytochrome c oxidase subunit 1(cox1) gene were performed for characterizing the liver hydatid cysts of sheep and cows in Iraq. The finding showed that all samples belonged to the G1 sheep strain (Abdulla et al 2020). Based on the reports of Thompson (1986), Rausch (1997), andRoming et al (2015), E. granulosus belongs to the following classification: Phylum: Platyhelminthes.…”

Molecular differentiation of Thysaniezia (Helictometra) giardi and Moniezia species based on 18s rRNA gene in small ruminants

“…Nevertheless, the molecular techniques revealed two different clades of T. ovilla from sheep and T. connochaeti from cattle. This suggests that using morphological methods is not useful for all cases in which certain molecular approaches should be followed for complete and accurate diagnosis (15)(16)(17).…”