Molecular characterization of field isolates of Pseudomonas syringae pv. glycinea differing in coronatine production

Abstract

Coronatine-producing and non-producing strains of Pseudomonas syringae pv. gfycinea have been examined. We found a connection between copper resistance and synthesis of coronatine. Published data implied that these properties may be encoded on different plasmids. Production of coronatine and copper resistance were also found to be correlated for pv. glycinea in 19 field-isolates from leaf spots of plants in a soybean field and in 28 strains of a bacterial culture collection. Genomic diversity within pv. glycinea was investigated by plasmid profiling, DNA hybridization studies and PCR analysis. All strains unable to produce coronatine (cor-) were sensitive to copper ions and showed no homology to DNA from plasmid pSAY1, which carries a gene cluster for steps in coronatine production. In addition, cor-strains could be distinguished from coronatine-producing strains by a single unique band when amplified by random primer PCR. Plasmid profiles of strains isolated from field-populations during 1983, 1985 and 1990 showed that coronatine-producing and non-producing strains were present. The plasmid patterns also varied in 28 strains examined from a culture collection. No correlation between plasmid patterns and race specificity was observed. Cosmid pSAYl proved to be an effective probe for detection of the coronatine synthesis genes and also revealed polymorphisms in coronatine producing strains of pv. glycinea.

Characterization and Mutational Analysis of Three Allelic lsc Genes Encoding Levansucrase in Pseudomonas syringae

“…2B). A clear signal was detected with a band representing the approximately 60-kb plasmid p4180D (3,46). When plasmid DNA from PG4180 was digested with the restriction enzyme SalI, a 10.5-kb fragment hybridized to the probe (data not shown).…”

The use of PCR melting profile for typing of Pseudomonas syringae isolates from stone fruit trees

“…Although 4 other Psm race 1 isolates do not posses this gene, they appeared to be pathogenic to cherry fruitlets. Some COR-defective (COR -) mutants were still pathogenic, however, the severity of symptoms was significantly less than those for COR producers (Xu and Gross 1988;Ullrich et al 1993;Bender et al 1999). The yersiniabactin gene was detected only in Pseudomonas syringae pv.…”

Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen Pseudomonas syringae pv. maculicola ES4326

“…Numerous plasmid-encoded virulence genes have been identified, including the gene cluster that directs the biosynthesis of the phytotoxin coronatine. Coronatine enables P. syringae to grow to higher densities in planta and move systemically (7,8,14,50,52,81). P. syringae plasmids can also encode secreted virulence proteins referred to as type III effectors (2,11,13,30,39,45,64).…”