Molecular characterization of Fiji disease fijivirus genome segment 9.

Soo, Hui Meng; Handley, J. A.; Maugeri, Marco; Burns, Parichart; Smith, Grant R.; Dale, James L.; Harding, Robert M.

doi:10.1099/0022-1317-79-12-3155

Cited by 28 publications

(15 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 3a shows 22% identity and 39% similarity with an S2-encoded P2 protein of rice ragged stunt virus (RRSV), a member of the genus Oryzavirus (38), 17% identity and 33% similarity with an S4-encoded 130-kDa protein of Nilaparvata lugens reovirus (NLRV), a putative member of the genus Fijivirus (28), and 17% identity and 36% similarity with an S4-encoded protein of Fiji disease fijivirus (FDV), a member of the genus Fijivirus (37). There appears to be little sequence similarity between reoviruses of different genera (13).…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Bombyx mori Cytoplasmic Polyhedrosis Virus Genome Segment 4

Ikeda

Nagaoka

Winkler

et al. 2001

J Virol

View full text Add to dashboard Cite

The complete nucleotide sequence of the genome segment 4 (S4) of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) was determined. The 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kDa. Western blot analysis showed that S4 encodes BmCPV protein VP3, which is one of the outer components of the BmCPV virion. Sequence analysis of the deduced amino acid sequence of BmCPV VP3 revealed possible sequence homology with proteins from rice ragged stunt virus (RRSV) S2, Nilaparvata lugens reovirus S4, and Fiji disease fijivirus S4. This may suggest that plant reoviruses originated from insect viruses and that RRSV emerged more recently than other plant reoviruses. A chimeric protein consisting of BmCPV VP3 and green fluorescent protein (GFP) was constructed and expressed with BmCPV polyhedrin using a baculovirus expression vector. The VP3-GFP chimera was incorporated into BmCPV polyhedra and released under alkaline conditions. The results indicate that specific interactions occur between BmCPV polyhedrin and VP3 which might facilitate BmCPV virion occlusion into the polyhedra.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Bombyx mori Cytoplasmic Polyhedrosis Virus Genome Segment 4

Ikeda

Nagaoka

Winkler

et al. 2001

J Virol

View full text Add to dashboard Cite

show abstract

“…The functions of MRCV S5-S7 and S9-encoded proteins are still unknown [1,[9][10][11]. Nevertheless, PROSITE and Pfam-HMMs analysis of MRCV S7 and S9-encoded putative proteins allowed their classification within the group of non-structural fijivirus proteins [11], as suggested for Rice black streaked dwarf virus (RBSDV) S7 and S9, and Fiji disease virus (FDV) S9 ORF-2 [12,13]. With the exception of the major core capsid protein [14], encoded by MRCV S3, for which the subcellular localization has been experimentally demonstrated, function and cellular localization of proteins encoded by MRCV have not been elucidated.…”

Section: Introductionmentioning

confidence: 98%

Immunodetection and subcellular localization of Mal de Río Cuarto virus P9-1 protein in infected plant and insect host cells

et al. 2010

View full text Add to dashboard Cite

Mal de Río Cuarto virus (MRCV), a member of the genus Fijivirus, family Reoviridae, has a genome consisting of 10 dsRNA segments. The segment 9 (S9) possesses two non-overlapping open reading frames (ORF-1 and ORF-2) encoding two putative proteins, MRCV P9-1 and MRCV P9-2, both of unknown function. The MRCV S9 ORF-1 was RT-PCR amplified, expressed in pET-15b vector, and the recombinant protein produced was used to raise an antiserum in rabbit. Western blot with the specific MRCV P9-1 antiserum detected a protein of about 39 kDa molecular weight present in crude protein extracts from infected plants and insects. However, no reaction was observed when this antiserum was tested against purified virus. In contrast, only virus particles were detected by a MRCV-coat antiserum used as a validation control. These results suggest that MRCV S9 ORF-1 encodes a non-structural protein of MRCV. Immunoelectron microscopy assays confirmed these results, and localized the MRCV P9-1 protein exclusively in electron-dense granular viroplasms within the cytoplasm of infected plants and insects cells. As viroplasms are believed to be the replication sites of reoviruses, the intracellular location of MRCV P9-1 protein suggests that it might be involved in the assembly process of MRCV particles.

show abstract

“…The inserts from selected clones were subsequently sequenced. The terminal sequences of FDV S2, S4 and S7 were amplified using anchor-ligated PCR [14]. Amplicons were cloned into pGEM-T (Promega) or pGEM-3Zf(+) and were either sequenced directly or cloned into pBlueScript SK+ (Clonetech).…”

Section: Methodsmentioning

confidence: 99%

“…The complete sequence of seven FDV genome segments, S1, S3, S5, S6 and S8-S10 have been published [14][15][16]. This paper reports the molecular characterisation and possible gene functions of the remaining three FDV segments (S2, S4 and S7), and discusses the possible relationship of FDV to other reoviruses.…”

Section: Introductionmentioning

confidence: 96%

Molecular Analysis of Fiji Disease Virus Segments 2, 4 and 7 Completes the Genome Sequence

et al. 2006

View full text Add to dashboard Cite

The complete nucleotide sequences of Fiji disease virus (FDV) genome segments S2, S4 and S7 were determined. This now completes the sequencing of all ten dsRNA genome segments of the Fijivirus type member, FDV, which comprises a total of 29339 nt. FDV S2, S4 and S7 comprised 3820, 3568 and 2194 nt, respectively. S2 and S4 each contained a single open reading frame (ORF), which encoded putative proteins of 137 and 133 kDa, respectively, while S7 contained two ORFs, which encoded putative proteins of 42 and 37 kDa. The putative amino acid sequences of FDV S2 and S4 showed most similarity to the gene products of Rice black-streaked dwarf virus (RBSDV) S2 and RBSDV S3, respectively. The putative amino acid sequences of FDV S7 ORF I and II showed most similarity to Maize rough dwarf virus (MRDV) S6 ORF I and RBSDV S7 ORF II, respectively. Phylogenetic analyses showed that FDV was most closely related to the group 2 fijiviruses.

show abstract

Molecular characterization of Fiji disease fijivirus genome segment 9.

Cited by 28 publications

References 23 publications

Molecular Characterization of Bombyx mori Cytoplasmic Polyhedrosis Virus Genome Segment 4

Molecular Characterization of Bombyx mori Cytoplasmic Polyhedrosis Virus Genome Segment 4

Immunodetection and subcellular localization of Mal de Río Cuarto virus P9-1 protein in infected plant and insect host cells

Molecular Analysis of Fiji Disease Virus Segments 2, 4 and 7 Completes the Genome Sequence

Contact Info

Product

Resources

About