Molecular characterization of FMRFamide-like peptides in Meloidogyne graminicola and analysis of their knockdown effect on nematode infectivity

Kumari, C. Gyana; Rao, Uma; Chaudhary, Sakshi; Banakar, Prakash; Papolu, Pradeep K.; Rao, Uma

doi:10.1016/j.gene.2017.03.042

Cited by 26 publications

(33 citation statements)

References 44 publications

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“…Previously, we had reported the expression site of Mi-flp12 and Mi-flp18 in the nerve cells, involved in the various biological and physiological process of M. incognita J2s (Banakar et al, 2015;Banakar and Rao, 2016). These findings support the involvement of the three FLPs in locomotion, host finding, reproduction and other physiological roles in the nematodes (White et al, 1986;Kimber et al, 2002Kimber et al, , 2007Rogers et al, 2003;Johnston et al, 2010;Kumari et al, 2017).…”

Section: Discussionsupporting

confidence: 80%

“…rostochiensis, G. pallida and M. incognita and M. graminicola (Atkinson et al, 1988;Maule et al, 2002;McVeigh et al, 2005;Kimber et al, 2007;Dalzell et al, 2010;Holden-Dye and Walker, 2011;Atkinson L. E. et al, 2013;Papolu et al, 2013;McCoy et al, 2014;Kumari et al, 2017;Warnock et al, 2017). So far, nineteen FLPs have been identified in the M. incognita and out of which six have been established to have transcriptional evidence (Abad et al, 2008) and host-delivered RNAi of Mi-flp14 and Mi-flp18 in N. tabacum provided excellent reduction in nematode parasitism and reproductive potential (Papolu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Investigation on the FLPs genes function through RNAi has shown reduced migration, penetration and aberrant behavioral phenotypes in M. incognita, Meloidogyne graminicola, and Globodera pallida (Urwin et al, 2002;Kimber et al, 2007;Dalzell et al, 2010;Atkinson L. E. et al, 2013;Papolu et al, 2013;Dong et al, 2014;Banakar et al, 2015;Kumari et al, 2017). Further, effectiveness of in vitro RNAi of FLP genes (Gp-flp1, Gp-flp12, and Gp-flp18) in G. pallida (Kimber et al, 2007) and host-delivered RNAi of Mi-flp14, Mi-flp18 in M. incognita (Papolu et al, 2013) indicate that it would be intriguing to investigate the synergistic or additive impacts, if any, of concomitant knockdown of more than one flp gene.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simultaneous RNAi Knockdown of Three FMRFamide-Like Peptide Genes, Mi-flp1, Mi-flp12, and Mi-flp18 Provides Resistance to Root-Knot Nematode, Meloidogyne incognita

et al. 2020

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simultaneous RNAi Knockdown of Three FMRFamide-Like Peptide Genes, Mi-flp1, Mi-flp12, and Mi-flp18 Provides Resistance to Root-Knot Nematode, Meloidogyne incognita

et al. 2020

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“…graminicola has been reported in Oryza glaberrima and O . longistaminata , but limited resistant source has been reported in O. sativa ( Soriano et al., 1999 ; Cabasan et al., 2012 ; Kumari et al., 2016 ; Kumari et al., 2017 ; Hatzade et al., 2020 ). The recent advancements in PPN genomics and transcriptomics have enabled us to identify the propitious molecular targets in nematodes that can be exploited for their management and also for drug designing ( Danchin et al., 2013 ; Taylor et al., 2013 ; Shivakumara et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…These observations putatively indicated the potential of the genes as molecular target(s) against M. graminicola . Subsequently, Kumari et al. (2017) characterized nine flp genes from M. graminicola including flp-1 and flp-12 , and in vitro silencing of the said genes resulted in significantly reduced penetration of J2s and their infection potential in rice.…”

Section: Introductionmentioning

confidence: 99%

Host-Induced Silencing of FMRFamide-Like Peptide Genes, flp-1 and flp-12, in Rice Impairs Reproductive Fitness of the Root-Knot Nematode Meloidogyne graminicola

et al. 2020

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Rice ( Oryza sativa L.) is one of the major staple food crops of the world. The productivity of rice is considerably affected by the root-knot nematode, Meloidogyne graminicola . Modern nematode management strategies targeting the physiological processes have established the potency of use of neuromotor genes for their management. Here, we explored the utility of two FMRFamide like peptide coding genes, Mg-flp-1 and Mg-flp-12 of M. graminicola for its management through host-induced gene silencing (HIGS) using Agrobacterium- mediated transformation of rice. The presence and integration of hairpin RNA (hpRNA) constructs in transgenic lines were confirmed by PCR, qRT-PCR, and Southern and Northern hybridization. Transgenic plants were evaluated against M. graminicola , where phenotypic effect of HIGS was pronounced with reduction in galling by 20–48% in the transgenic plants. This also led to significant decrease in total number of endoparasites by 31–50% for Mg-flp - 1 and 34–51% for Mg-flp-12 transgenics. Likewise, number of egg masses per plant and eggs per egg mass also declined significantly in the transgenics, ultimately affecting the multiplication factor, when compared to the wild type plants. This study establishes the effectiveness of the two M. graminicola flp genes for its management and also for gene pyramiding.

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Micro RNA‐induced gene silencing strategy for the delivery of siRNAs targeting Meloidogyne incognita in a model plant Nicotiana benthamiana

Hada

Patil

Bajpai

et al. 2021

Pest Management Science

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BACKGROUND: Occurrence of multiple biotic stresses on crop plants result in drastic yield losses which may have severe impact on the food security. It is a challenge to design strategies for simultaneous management of these multiple stresses. Hence, establishment of innovative approaches that aid in their management is critical. Here, we have introgressed a micro RNAinduced gene silencing (MIGS) based combinatorial gene construct containing seven target gene sequences of cotton leaf curl disease (CLCuD), cotton leaf hopper (Amrasca biguttula biguttula), cotton whitefly (Bemisia tabaci) and root-knot nematode (Meloidogyne incognita).RESULTS: Stable transgenic lines of Nicotiana benthamiana were generated with the T-DNA harboring Arabidopsis miR173 target site fused to fragments of Sec23 and ecdysone receptor (EcR) genes of cotton leaf hopper and cotton whitefly. It also contained C2/replication associated protein (C2/Rep) and C4 (movement protein) along with ⊎C1 gene of betasatellite to target CLCuD, and two FMRFamide-like peptide (FLP) genes, Mi-flp14 and Mi-flp18 of M. incognita. These transgenic plants were assessed for the amenability of MIGS approach for pest control by efficacy evaluation against M. incognita. Results showed successful production of small interfering RNA (siRNA) through the tasiRNA (trans-acting siRNA) pathway in the transgenic plants corresponding to Mi-flp18 gene. Furthermore, we observed reduced Mi-flp14 and Mi-flp18 transcripts (up to 2.37 ± 0.12-fold) in females extracted from transgenic plants. The average number of galls, total endoparasites, egg masses and number of eggs per egg mass reduced were in the range 27-62%, 39-70%, 38-65% and 34-49%, respectively. More importantly, MIGS transgenic plants showed 80% reduction in the nematode multiplication factor (MF).CONCLUSION: This study demonstrates successful validation of the MIGS approach in the model plant, N. benthamiana for efficacy against M. incognita, as a prelude to translation to cotton.

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Molecular characterization of FMRFamide-like peptides in Meloidogyne graminicola and analysis of their knockdown effect on nematode infectivity

Cited by 26 publications

References 44 publications

Simultaneous RNAi Knockdown of Three FMRFamide-Like Peptide Genes, Mi-flp1, Mi-flp12, and Mi-flp18 Provides Resistance to Root-Knot Nematode, Meloidogyne incognita

Simultaneous RNAi Knockdown of Three FMRFamide-Like Peptide Genes, Mi-flp1, Mi-flp12, and Mi-flp18 Provides Resistance to Root-Knot Nematode, Meloidogyne incognita

Host-Induced Silencing of FMRFamide-Like Peptide Genes, flp-1 and flp-12, in Rice Impairs Reproductive Fitness of the Root-Knot Nematode Meloidogyne graminicola

Micro RNA‐induced gene silencing strategy for the delivery of siRNAs targeting Meloidogyne incognita in a model plant Nicotiana benthamiana

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