Molecular Characterization of Fusarium Oxysporum F. Sp. Cubense of Banana

Abstract

Problem statement: Morphological characterization of Fusarium species which emphasize on microscopic and cultural characteristics are not sufficient to characterize Fusarium Oxysporum F. sp. Cubense (FOC) from banana as these characteristics could easily influence by environmental factors. As an alternative molecular methods were used to characterize and to assess genetic variation of FOC from different banana cultivars. Knowledge on the genetic variation is important to determine the genetic relationship between FOC isolates from different banana cultivars. Approach: Two PCRbased methods, Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) and restriction analysis of the Internal Transcribed Spacer and 5.8S regions (ITS+5.8S regions) were used to characterize Fusarium Oxysporum F. sp. Cubense (FOC) isolates from different banana cultivars. The genetic relationship of the FOC isolates were analyzed using Un-weighted Pair-Group Method with Arithmetic Averages (UPGMA) cluster analysis based on Jaccard Similarity Coefficient. Results: Restriction patterns of the ITS+5.8S regions using nine restriction enzymes namely, Alu I, Eco RI, Eco 88I, Bsu RI, Bsu 15I, Hin fI, Hin 6I, Msp I and Taq I and ERIC-PCR showed low variation among the FOC isolates studied, indicating close relationship among the isolates. Unweighted Pair-group Method with Arithmetic Averages (UPGMA) cluster analysis based on Jaccard Similarity Coefficient showed that the FOC isolates were grouped into two main clusters with similarity value of 41.4-100% in PCR-RFLP of ITS + 5.8S and 45-100% similarity based on ERIC-PCR analysis, respectively. Cluster analysis of the combined data also showed that the FOC isolates were grouped into two clusters, sharing 42.9-100% similarity. Conclusion/Recommendations: The results of the present study indicate that the FOC isolates were closely related regardless of banana cultivars and location.

Variabilidade genética de isolados de Fusarium oxysporum f. sp. cubense obtidos de bananais do norte de Minas Gerais

“…Sugeriram ainda que esta variação genética poderia ser devido à adaptação e coevolução do fungo com o hospedeiro e com os fatores ambientais do local. Leong et al (2009) …”

Diversity and identity of Fusarium species occurring on fruits, vegetables and food grains

“…ciceris species recovered from wilted chickpea plants using ITS-1 and ITS-4 species specific primers and sequenced. Similarly, other studies carried out by Leong et al (2009), Dissanayake et al (2009), Palmore et al (2010, Chehri et al (2011) on identification of different Fusarium species using ITS-rDNA as a molecular marker provide evidences that this marker was helpful for the identification of Fusarium species. Our findings corroborate with these studies, which confirmed that ITS-rDNA markers can be used for the rapid and accurate identification of Fusarium species.…”

“…Banana (Musa sapientum) is susceptible to the infection by several fungal diseases of which Fusarium-wilt caused by F. oxysporum is considered to be the most important and responsible for severe economic losses in bananaproducing countries (Butler 2013;Moretti et al 2004). Leong et al (2009) also reported the infection caused by F. oxysporum in banana and successfully isolated 13 isolates of F. oxysporum from Malaysia and two isolates from Indonesia. Recently, Triest et al (2016) reported that F. musae was responsible for the infection of banana fruits and the consumption of such infected fruits may cause fusariosis in human being.…”

Effects of iron and boron combinations on the suppression of Fusarium wilt in banana

“…cubense (FOC) is a serious vascular disease. The pathogen is divided into four races based on differences in pathogenicity on Musa cultivars; race 4 is ranked as the most virulent race and can infect all Cavendish cultivars1. Fusarium oxysporum can survive for long periods in the absence of the host, mainly in the form of thick-walled chlamydospores.…”