Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis

Goswami, Lalit; Prasad, Pramod Kumar; Tandon, Veena; Chatterjee, Anupam

doi:10.1007/s00436-009-1354-8

Cited by 25 publications

(9 citation statements)

References 23 publications

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“…Previous studies have used the easily obtained ITS2 sequence as a molecular marker to distinguish among paramphistomoid species (Itagaki et al 2003 ; Rinaldi et al 2005 ; Goswami et al 2009 ; Lotfy et al 2010 ; Sanabria et al 2011 ; Ichikawa et al 2013 ; Shylla et al 2013 ; Ghatani et al 2014 ; Dube et al 2015 ). ITS2 is helpful for distinguishing paramphistomoid genera and differentiating more divergent species within a genus (Rinaldi et al 2005 ; Ghatani et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Loads of trematodes: discovering hidden diversity of paramphistomoids in Kenyan ruminants

et al. 2016

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SUMMARYParamphistomoids are ubiquitous and widespread digeneans that infect a diverse range of definitive hosts, being particularly speciose in ruminants. We collected adult worms from cattle, goats and sheep from slaughterhouses, and cercariae from freshwater snails from ten localities in Central and West Kenya. We sequenced cox1 (690 bp) and internal transcribed region 2 (ITS2) (385 bp) genes from a small piece of 79 different adult worms and stained and mounted the remaining worm bodies for comparisons with available descriptions. We also sequenced cox1 and ITS2 from 41 cercariae/rediae samples collected from four different genera of planorbid snails. Combining morphological observations, host use information, genetic distance values and phylogenetic methods, we delineated 16 distinct clades of paramphistomoids. For four of the 16 clades, sequences from adult worms and cercariae/rediae matched, providing an independent assessment for their life cycles. Much work is yet to be done to resolve fully the relationships among paramphistomoids, but some correspondence between sequence- and anatomically based classifications were noted. Paramphistomoids of domestic ruminants provide one of the most abundant sources of parasitic flatworm biomass, and because of the predilection of several species use Bulinus and Biomphalaria snail hosts, have interesting linkages with the biology of animal and human schistosomes to in Africa.

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Section: Discussionmentioning

confidence: 99%

Loads of trematodes: discovering hidden diversity of paramphistomoids in Kenyan ruminants

et al. 2016

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“…Because of such problems, the development of alternative approaches to better delineate species in this group is needed. PCR-based techniques providing rDNA ITS2 sequences have proven to be a reliable tool to identify digenean species and to recover their phylogenetic relationships (Blair et al, 1999;Itagaki et al, 2003;Reinitz et al, 2007;Lotfy et al, 2008;Goswami et al, 2009;Van Van et al, 2009). Moreover, ITS2 has been found to be a useful marker for species identification of paramphistomes (Itagaki et al, 2003;Rinaldi et al, 2005;Goswami et al, 2009).…”

Section: Introductionmentioning

confidence: 98%

A molecular approach for identification of paramphistomes from Africa and Asia

Lotfy

Brant

Ashmawy

et al. 2010

Veterinary Parasitology

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“…In recent years, several molecular tools including sequence data related to conserved domains have unveiled their utility in molecular identification, taxonomy, systematics and phylogeny of floral, faunal and microbial species, too (Huang et al 2006;Prasad et al 2009a, b;Wiemers et al 2009;Yao et al 2010;Sharma et al 2012). Since Woese and Fox (1977), the nuclear ribosomal repeat cistrons have also been widely implied for parasites (Littlewood and Olson 2001;Waeschenbach et al 2007;Jyrwa et al 2009;Goswami et al 2009;Tandon et al 2010;Prasad et al 2011;Ghatani et al 2012). Molecular phylogeny has advanced our understanding of comparative studies in basic and applied biology and more generally of their position within the phylum Platyhelminthes (Brooks and Hoberg 2001;Tandon et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA internal transcribed spacer 2 region

et al. 2012

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The nodular tapeworm, Raillietina echinobothrida is a well studied avian gastrointestinal parasite of family Davaineidae (Cestoda: Cyclophyllidea). It is reported to be the largest in size and second most prevalent species infecting chicken in north-east India. In the present study, morphometrical methods coupled with the molecular analysis of the second internal transcribed spacer (ITS2) region of ribosomal DNA were employed for precise identification of the parasite. The annotated ITS2 region was found to be 446 bp long and further utilized to elucidate the phylogenetic relationships and its species-interrelationships at the molecular level. In phylogenetic analysis similar topology was observed among the trees obtained by distance-based neighbor-joining as well as character-based maximum parsimony tree building methods. The query sequence R. echinobothrida is well aligned and placed within the Davaineidae group, with all Raillietina species well separated from the other cyclophyllidean (taeniid and hymenolepid) cestodes, while Diphyllobothrium latum (Pseudophyllidea: Diphyllobothriidae) was rooted as an out-group. Sequence similarities indeed confirmed our hypothesis that Raillietina spp. are neighboring the position with other studied species of order Cyclophyllidea against the out-group order Pseudophyllidea. The present study strengthens the potential of ITS2 as a reliable marker for phylogenetic reconstructions.

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Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis

Cited by 25 publications

References 23 publications

Loads of trematodes: discovering hidden diversity of paramphistomoids in Kenyan ruminants

Loads of trematodes: discovering hidden diversity of paramphistomoids in Kenyan ruminants

A molecular approach for identification of paramphistomes from Africa and Asia

Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA internal transcribed spacer 2 region

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