1995
DOI: 10.1007/bf00020464
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Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress

Abstract: A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 49.4% and 58.5% identity with glyoxalase-I isolated from bacteria and human, respectively. Furthermore, yeast cells expressing GLX1 showed a glyoxalase-I specific activity 20-fold higher than non-t… Show more

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Cited by 163 publications
(109 citation statements)
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“…Under stress conditions, the increased level of methylglyoxal could further lower the glutathione level required for scavenging of reactive oxygen species, which increase under stress conditions [25]. Since the glyoxalase I has been shown to be upregulated under different abiotic stresses [14,15] and an increase in the glyoxalase II transcript has been observed under various abiotic stresses in Brasssica juncea [17] and in rice [18], the overexpression of the enzymes of the glyoxalase system is presumed to be involved in stress tolerance by detoxifying methylglyoxal and elevating the level of glutathione. This has been recently demonstrated in transgenic tobacco plants overexpressing the glyoxalase I and glyoxalase II gene.…”
Section: Resultsmentioning
confidence: 99%
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“…Under stress conditions, the increased level of methylglyoxal could further lower the glutathione level required for scavenging of reactive oxygen species, which increase under stress conditions [25]. Since the glyoxalase I has been shown to be upregulated under different abiotic stresses [14,15] and an increase in the glyoxalase II transcript has been observed under various abiotic stresses in Brasssica juncea [17] and in rice [18], the overexpression of the enzymes of the glyoxalase system is presumed to be involved in stress tolerance by detoxifying methylglyoxal and elevating the level of glutathione. This has been recently demonstrated in transgenic tobacco plants overexpressing the glyoxalase I and glyoxalase II gene.…”
Section: Resultsmentioning
confidence: 99%
“…An inverse correlation between glyoxalase I activity and differentiation in vitro was also observed in plants [12,13]. Glyoxalase I from tomato and Brassica juncea has been shown to be upregulated under different abiotic stresses [14,15]. Moreover; the overexpression of glyoxalase I from B. juncea has been shown to impart tolerance in tobacco plants under salinity and heavy metal stress [15].…”
Section: Introductionmentioning
confidence: 99%
“…The physiological significance of the glyoxalase system has not been clearly defined in plants; however, this system has been often regarded as a ''marker for cell growth and division'' (11). Gly I has been shown to be up-regulated in tomato in response to salt and osmotic stress and to phytohormonal stimuli (12).…”
mentioning
confidence: 99%
“…When plant exposed to stress conditions, enzymes of glycolysis and TCA cycles cells showed increased activity due to rapid functioning of cell (Umeda et al, 1994;Espartero et al, 1995;Sommer et al, 2001) and as a result increased triose phosphates are converted to MG instead of pyruvate only. So higher amount of methylglyoxal production under drought and salinity stresses confirmed by , Alam et al (2014) and Nahar et al (2015).…”
Section: Figmentioning
confidence: 99%
“…During conversion of glyceraldehyde 3-phosphate (G3P) from dihydroxyacetone phosphate (DHAP) in glycolysis, MG is formed spontaneously in plants by non-enzymatic mechanisms under physiological conditions (Espartero et al, 1995;Yadav et al, 2005a). The rate of glycolysis increases under stress conditions, leading to an imbalance (in the initial and latter five reactions) in the pathway.…”
Section: Introductionmentioning
confidence: 99%