Molecular Characterization of Hairy Fleabane Using Rapd

Silva, D.R.O.; Agostinetto, Dirceu; Várgas, Leandro

doi:10.1590/s0100-83582016340300004

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…Em plantas daninhas, esta técnica é atrativa em um primeiro momento, pois não se necessita conhecer sobre a sequência nucleotídica da planta, fato comum às plantas daninhas. A técnica RAPD foi eficiente para agrupar diferentes biótipos de Conyza bonariensis devido a variabilidade genética, além de que todos os biótipos são mesmo da mesma espécie apresentado variedade distinta (Silva et al, 2016).…”

Section: Marcadores Molecularesunclassified

Biologia molecular aplicada à ciência das plantas daninhas

et al. 2018

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Resumo -As plantas daninhas possuem elevada variabilidade genética, e principalmente por este motivo, são adaptadas a ambientes com intensa atividade humana. Embora o controle de plantas daninhas tenha evoluído de maneira positiva nos últimos anos, elas continuam a interferir na produção agrícola. O objetivo desta revisão bibliográfica é apresentar a contribuição da biologia molecular nos estudos aplicados a herbologia. Há lacunas entre o que aprendemos sobre genômica de plantas daninhas e como esses conhecimentos poderiam nos auxiliar no manejo e melhorar a competividade de culturas agrícolas frente às plantas daninhas. Muitos estudos na área da ciência das plantas daninhas podem ser realizados com o emprego de técnicas de biologia molecular, sendo eles: caracterização do genoma de espécies de plantas daninhas, visando à identificação destes com maior acurácia, identificação de espécies resistentes a herbicidas e seu mecanismo de resistência, variabilidade e similaridade genética entre populações de plantas daninhas, identificação de genes envolvidos nos processos de interação entre plantas, dentre outros. Palavras-chave: herbicidas, variabilidade genética, genômica, DNA, biotecnologia Abstract -The weeds have high genetic variability, mainly for this, they are adapted to environments disturbed by humans. Although weed control has evolved positively in recent years, they continue to interfere with agricultural production. The objective of this bibliographic review is to present the contribution of molecular biology in the studies applied to herbology. There area gap in what we learn about weed genomics, which could help us manage and improve the competitiveness of weed crops. Many studies in the field of weed science can be carried out using molecular biology techniques. The characterization of the genome of weed species, aiming to identify species with higher accuracy, identification of herbicide resistant species and its mechanism of resistance, genetic variability and similarity among weed populations, identification of genes involved in the interaction processes between plants, among other.

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Section: Marcadores Molecularesunclassified

Biologia molecular aplicada à ciência das plantas daninhas

et al. 2018

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“…In Brazil, horseweed and hairy-fleabane (C. bonariensis) were first reported as GR weeds in 2005 [12], followed by Sumatran fleabane (C. sumatrensis) in 2010 [9]. Despite the importance of all Conyza species in Brazilian agricultural fields, molecular analyses demonstrated that GR C. bonariensis predominates in Southern Brazil [13]. Currently, the estimated area of GR Conyza spp.…”

Section: Introductionmentioning

confidence: 99%

Glyphosate and Saflufenacil: Elucidating Their Combined Action on the Control of Glyphosate-Resistant Conyza bonariensis

et al. 2020

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Synergic effects of glyphosate and saflufenacil have been reported to control Conyza spp. However, the mechanism of this synergic combination is unknown. The objectives of this study were to investigate the effects of the combination of glyphosate and saflufenacil on the control and resistance status of glyphosate-resistant (GR) C. bonariensis, as well as on oxidative stress and lipidic peroxidation. Glyphosate-resistant and glyphosate-sensitive (GS) biotypes were treated with different rates of saflufenacil, glyphosate, and glyphosate combined with saflufenacil. The combination of glyphosate (1480 g ae ha−1) and saflufenacil (≥15 g ha−1) presented the best control of GR plants compared with their individual effects. It also reduced the resistance factor from 19.6 to 4.6 (4.3-fold) and represents a good alternative for resistance management. The combination of glyphosate and saflufenacil resulted in higher oxidative stress and lipidic peroxidation compared with the effects of either herbicide alone. The oxidative stress and lipid peroxidation promoted by the combination of the herbicides occurred earlier after treatment and remained at higher levels over a longer period (12–192 h) compared with their individual results. The combined oxidative stress and lipid peroxidation at high levels over a long time is likely to be one reason that the combination of glyphosate and saflufenacil effectively controls GR C. bonariensis.

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“…(Souza et al, 2008); sugar apple (Annona squamosa L.) (Bharad;Kulwal;Bagal, 2009;Ahmad et al, 2010;Guimarães et al, 2013); Aloe ssp. (Shahira et al, 2016); Conyza bonariensis (Silva;Agostinetto;Vargas, 2016) and Abelmoschus species (Pravin;Shrikant;Venkataraman, 2018). The existence of the most recent molecular marker techniques does not underestimate the RAPD utility as a tool for the development of strategies, for the conservation of individuals and their seed collections (Santana et al, 2008;Gois, 2014a;Belarmino et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Forest inventory and the genetic diversity of the remaining fragment of Hymenaea courbaril L.

et al. 2018

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Hymenaea courbaril is a rare species in the riparian areas of the state of Sergipe, Brazil. This species is known as Brazilian cherry or Brazilian copal and it occurs in fragments of the Rainy Forest and in the transition zones between the Rainy Forest and the Caatinga Biomes. This work was carried out, in order to quantify the genetic diversity of the remaining population of Brazilian cherry, by RAPD markers. In a studied area of 100 ha in the low course of the São Francisco River, only 15 individuals were observed. The analyzed parameters were as follows: Jaccard’s genetic similarity, the number of observed alleles, the number of effective alleles, the genetic diversities of Nei, the percentages of the polymorphic loci, the genetic diversities when using the Shannon index and the coancestry coefficient. There was no correlation between the genetic distances and the geographical distances. Despite the low number of individuals, high genetic diversity was observed, a fact that contributes to the development of strategies for the conservation of the species.

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Molecular Characterization of Hairy Fleabane Using Rapd

Cited by 3 publications

References 27 publications

Biologia molecular aplicada à ciência das plantas daninhas

Biologia molecular aplicada à ciência das plantas daninhas

Glyphosate and Saflufenacil: Elucidating Their Combined Action on the Control of Glyphosate-Resistant Conyza bonariensis

Forest inventory and the genetic diversity of the remaining fragment of Hymenaea courbaril L.

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