Molecular Characterization of Hand Flora and Environmental Isolates in a Community Setting

Pancholi, Preeti; Healy, Mimi; Bittner, Tracy; Webb, Renee; Wu, Fan; Aiello, Allison E.; Larson, Elaine; Latta, Phyllis Della

doi:10.1128/jcm.43.10.5202-5207.2005

Cited by 19 publications

(7 citation statements)

References 40 publications

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“…Case 3 had pre-necrotizing enterocolitis with increased intestinal permeability that could favor passage of the bacteria to the blood. A recent study in a community setting revealed that P. agglomerans was isolated from the hands and environment of individuals in households where it might serve as a potential pathogen for either community-or hospital-acquired infections [11] . In analogy, the hands of healthcare workers might offer transient carriage for the organism though, in our case, such speculation was not confirmed.…”

Section: Discussionmentioning

confidence: 99%

<i>Pantoea agglomerans</i> Bloodstream Infection in Preterm Neonates

Aly

Salmeen

Lila³

et al. 2008

Med Princ Pract

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Objective: To report an uncommon incidence of sporadic bloodstream infection (BSI) caused by Pantoea agglomerans in preterm neonates. Case Presentation and Intervention: Fives cases of nosocomial BSI with P. agglomerans in preterm neonates (weight ≤1,500 g; age 8–17 days; gestational age 26–30 weeks) are presented. All cases were late onset neonatal sepsis (>7 days of age). Lethargy, skin mottling and bradycardia were often present. Although there was no evidence of pneumonia, desaturation was a common feature. Thrombocytopenia developed in 4 patients, metabolic acidosis in 2 and jaundice in 2. No bleeding tendency or disseminating intravascular coagulation was recorded. Organisms cultured from blood were identified by the Vitek-2 system (bioMérieux, France) and the findings confirmed by testing the isolate on the API 20E system. All isolates shared in vitro susceptibility to gentamicin, amikacin, ciprofloxacin, piperacillin/tazobactam and meropenem. One patient was treated with a cefotaxime/amikacin combination, 2 with meropenem and the remaining 2 with tazocin. All patients responded well to antibiotic treatment and survived. Conclusion:P. agglomerans is an unusual pathogen in the etiology of neonatal sepsis. Despite significant clinical deterioration, early detection and proper antibiotic therapy carry a favorable outcome.

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Section: Discussionmentioning

confidence: 99%

<i>Pantoea agglomerans</i> Bloodstream Infection in Preterm Neonates

Aly

Salmeen

Lila³

et al. 2008

Med Princ Pract

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“…Other studies identified hand flora in both community and hospital settings (Blank 1965, Williamson 1965, Larson et al. 2000, Pancholi et al. 2005) and scrubbing time was tested for its ability to reduce microbial counts (Pereira et al.…”

Section: Water Quality Hand Flora and Behaviourmentioning

confidence: 99%

A review of hand‐washing techniques in primary care and community settings

Smith

2009

Journal of Clinical Nursing

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“…We compared two solutions of S. aureus of different concentrations by colonizing the surface of a blood culture bottle (while instilling sterile fluids), which simulates better the real daily practice, instead of instilling a contaminated fluid directly through the catheter. Besides, we tested not only a known concentration of a single known microorganism, but also the microorganisms from the flora of the manipulators' hands, as it has been demonstrated that these are highly colonized and could be a potential source of contamination [ 12 ]. With this model based on a simple daily handling, we have demonstrated that manipulation of connectors using gloves impregnated with a 0.05 McFarland solution of S. aureus while instilling sterile saline had longer times of positivity, allowing us to prove this model in the comparison of other types of connectors with time enough to detect differences between them regarding their efficacy to prevent the entry of microorganisms through the catheter.…”

Section: Resultsmentioning

confidence: 99%

A simple and easy in vitro model to test the efficacy of IV lines' needleless connectors against contamination

Guembe

Pérez‐Granda

Alcalá

et al. 2014

ICMx

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BackgroundHub colonization after manipulation is responsible for 29% to 60% of catheter-related bloodstream infections (C-RBSI). Prevention can be achieved by the use of hub connectors, but its efficacy is generally based on instillation of high concentrations of microorganisms, which do not reflect the real contamination in daily practice. Our purpose was to create an in vitro model lasting long enough to be used for the comparison of the efficacy between various connectors against contamination simulating the real daily handling.MethodsThe model consisted of 40 blood culture bottles with an inserted cannula with a needle-free closed connector. Twice a day, each line was manipulated while instilling 1 mL of two different fluids (saline and propofol). We manipulated the bottles as follows: ten bottles with clean gloves and disinfecting connectors with alcohol (controls), ten bottles with hands (no gloves), ten bottles with gloves impregnated with a 0.5 McFarland (MF) solution of Staphylococcus aureus (SA), and ten bottles with gloves impregnated with a 0.05 MF solution of SA. The bottles were incubated in a BACTEC System at 37°C under continuous agitation up to 10 days. When a bottle turned positive, 100 μL of the fluid was cultured and incubated followed by microorganism identification using standard procedures.ResultsOverall, all bottles in the control group were negative at the end of the incubation time. In the three contamination experiments, almost all (38/40) bottles were positive during the incubation time. We only found differences regarding the median time to positivity (interquartile range (IQR)) between saline and propofol in the manipulation with SA 0.05 MF: 240 h (154.82 to 360.00) vs. 66 h (58.01 to 69.11), p = 0.008.ConclusionsA daily connector handling with 0.05 McFarland S. aureus solution while instilling saline proved to be a useful model lasting long enough to be used for the comparison of the efficacy of different types of closed needleless connectors against contamination.Electronic supplementary materialThe online version of this article (doi:10.1186/s40635-014-0027-9) contains supplementary material, which is available to authorized users.

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Molecular Characterization of Hand Flora and Environmental Isolates in a Community Setting

Cited by 19 publications

References 40 publications

<i>Pantoea agglomerans</i> Bloodstream Infection in Preterm Neonates

<i>Pantoea agglomerans</i> Bloodstream Infection in Preterm Neonates

A review of hand‐washing techniques in primary care and community settings

A simple and easy in vitro model to test the efficacy of IV lines' needleless connectors against contamination

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