2010
DOI: 10.1007/s10658-010-9703-0
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Molecular characterization of Hawaiian Sugarcane yellow leaf virus genotypes and their phylogenetic relationship to strains from other sugarcane-growing countries

Abstract: Sugarcane yellow leaf virus (SCYLV) is the causal agent of the sugarcane disease Yellow leaf (YL), which was first reported in Hawaii. The presence of SCYLV was detected by tissue blot immunoassay and the Hawaiian sugarcane cultivars fell into susceptible cultivars (with SCYLV) and resistant cultivars (without SCYLV). RT-PCR showed recently that the resistant cultivars also contain the virus, however with a 100-fold lower virus titre than in the susceptible cultivars. SCYLV is present as whole genome (6 kb) or… Show more

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Cited by 32 publications
(41 citation statements)
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“…It is well known that the topology of a SCYLV phylogenetic tree will be slightly varied depending on the targeted region (gene or the whole genome) (ElSayed et al 2011;Lin et al 2014). This difference in clustering could be due to the fact that the SCYLV genes used for phylogenetic analysis have experienced different evolutionary histories (Lin et al 2014).…”
Section: Discussionmentioning
confidence: 99%
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“…It is well known that the topology of a SCYLV phylogenetic tree will be slightly varied depending on the targeted region (gene or the whole genome) (ElSayed et al 2011;Lin et al 2014). This difference in clustering could be due to the fact that the SCYLV genes used for phylogenetic analysis have experienced different evolutionary histories (Lin et al 2014).…”
Section: Discussionmentioning
confidence: 99%
“…Among these ORFs, ORF0 encodes the P0 protein, which is an RNA-silencing suppressor and plays roles in viral pathogenicity and the expression of disease symptoms after infection (Mangwende et al 2009). So far, at least 10 SCYLV genotypes have been reported based on phylogenetic analysis at full genome or individual gene levels and geographic origins: BRA (from Brazil), PER (from Peru), CUB (from Cuba), REU (from Réunion Island), HAW (from Hawaii), COL (from Colombia), IND (from India), CHN1, CHN2, and CHN3 (from China) (Abu Ahmad et al 2006a, b;ElSayed et al 2011;Moonan and Mirkov 2002;Gao et al 2012;Chinnaraja et al 2013;Wang and Zhou 2010;Wang et al 2012;Lin et al 2014). SCYLV genotypes from different geographical locations may have highly heterogeneous sequences at genome level (Lin et al 2014) and distinguishing virulence characteristics by aphid transmission assays (Abu Ahmad et al 2007).…”
Section: Introductionmentioning
confidence: 99%
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“…In addition, the so-called resistant cultivars (H78-4153 and H78-7750), which previously were thought to be virus-free based on a tissue blot immunoassay (TBIA) (Schenck and Lehrer 2000), also contained the virus. Obviously, the immunological assay is less sensitive than RT-PCR, and only the cultivar line that was generated by meristem tip culture was indeed virus-free (ElSayed et al 2011). The ORF0 fragment was amplified in all SCYLV-preparations and revealed high virus titers.…”
Section: Discussionmentioning
confidence: 99%
“…The deduced amino acid sequence of P0 proteins is highly conserved among different geographic isolates of SCYLV (Abu Ahmad et al 2006;ElSayed et al 2011). However, the amino acid sequence identity between the P0s of poleroviruses is very low.…”
Section: Introductionmentioning
confidence: 99%