Molecular characterization of heat shock protein 70 gene in Iraqi buffalo

Habib, Hassan Nima

doi:10.33899/ijvs.2019.125633.1116

Cited by 6 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, Sosa et al (17) reported three nucleotide substitutions in GnRHR gene associated with ovarian inactivity in Egyptian buffaloes. So the genetic variation is needed for buffalo selection (34).…”

Section: Discussionmentioning

confidence: 99%

GnRHR gene polymorphism and its correlation with semen quality in Buffalo bulls (Bubalus bubalis)

Mahmoud¹,

Sakr²,

Ibrahim³

et al. 2021

IJVS

View full text Add to dashboard Cite

Fresh semen from fifty bulls was evaluated for ejaculate volume, individual motility, concentration, lives sperm and abnormalities as well as acrosome integrity. Bulls were classified according to semen motility into two groups; good and poor. DNA was extracted from semen of both groups, then the PCR followed by single-strand conformation polymorphism (SSCP) techniques were performed for mutation detection in gonadotropin releasing hormone receptor (GnRHR) gene through multiple sequence alignment. The results showed that the percentages of sperm motility, sperm concentration and live sperm, were significantly higher in good than poor semen quality bulls. However, semen volume, percentage of acrosome integrity and abnormalities did not differ between good and poor. The PCR amplification of 240-bp fragment and the results of SSCP appeared a genetic polymorphism with two patterns. Pattern I was seen in all good bulls, with incidence of 100%. Pattern II appeared only in poor semen quality bulls with a frequency of 31.25%. The sequence analysis of the PCR product for the two patterns showed two single nucleotide polymorphisms (SNPs) as a transversion base substitution mutation at positions 20 (T/A) and 193 (A/T). The GnRHR gene could be used as a genetic marker related to semen quality in buffalo due to the good semen bulls had a unique pattern.

show abstract

Section: Discussionmentioning

confidence: 99%

GnRHR gene polymorphism and its correlation with semen quality in Buffalo bulls (Bubalus bubalis)

Mahmoud¹,

Sakr²,

Ibrahim³

et al. 2021

IJVS

View full text Add to dashboard Cite

show abstract

“…The HSP70 protein, an important member of HSP family, is used as a significant indicator and molecular marker of adaptation to harsh environmental stress, since it is the most excitable protein against environmental stress among all HSPs. This protein has a variety of functions, such as resistance to stress and disease, protecting living cells and ensuring their survival (Patir and Upadhyay, 2010;Habib, 2020). In addition, the HSP70 protein is used as a selection parameter in developing of heat stress resistant breeds in cattle.…”

Section: Heat Tolerance and Genesmentioning

confidence: 99%

Physiological and Pyhsical Responses of Dairy Cattle to Heat Stress

Okuyucu

Akdağ

Erdem

et al. 2023

Black Sea Journal of Agriculture

View full text Add to dashboard Cite

Herd management and nutrition strategies against increasing negative effects of global warming on farm animals have been the subject of significant debates in recent years. The fact that the changes in the environmental conditions are directly related to the habitats of the animals and the conditions inside the barn can affect the farm animals negatively. Although effects of heat stress differ according to species, especially high-yielding dairy cattle exposed to heat stress, respond with various interactive mechanism such as physiological, biochemical, immunological, anatomical and behavioral. Therefore, with the selection practices that have been going on for many years to improve the yield characteristics of the animals significantly increased. In this respect, the increased heat load in the body of dairy cattle due to the high productivity decreases their tolerance to environmental conditions. This situation adversely affects the productivity of cows with high breeding value. Yield losses, varying according to some factors about heat stress, can be partially explained by decreasing feed intake as a result of a series of hormonal responses affecting appetite region. However, the physiological requirements of cattle must be defined in order to develop appropriate strategies to reduce or eliminate the negative effects of heat stress. In this review, the variations in physiological, biochemical and behavioral mechanisms originating from heat stress in dairy cattle and the care, nutrition and her management strategies that can be applied to eliminate or reduce the negative effects were discussed.

show abstract

“…In goats, the gene coding for the HSP70 protein comprises 1926bp nucleotide sequences corresponding with 641 amino acids and is located on chromosome 23 (Gade et al, 2010). Although HSP70 genes are highly conserved molecules (Pawar et al, 2013), several studies suggest the possibility of genetic polymorphisms of the HSP70 gene [Fatima et al (2019) and Nikbin et al (2014) in goat; Habib et al (2017) in bulls; Habib et al (2018a) in rams; Habib (2020) in buffaloes and Habib et al (2020) in poultry]. Iraqi local goats generally suffer from many problems directly affecting their production.…”

Section: /9mentioning

confidence: 99%

Genetic profiling of HSP70 gene in local Iraqi goats

2022

View full text Add to dashboard Cite

Animals display numerous physiological and behavioral responses that reduce the effects of heat stress. Moreover, genetic variance is strongly associated with responses to heat stress, including variants of heat shock proteins (HSPs) that are necessary for thermoregulation and stress resistance. Herein, we performed the molecular profiling of the HSP70 gene, and its polymorphism was demonstrated as a possible factor in the stress tolerance of local Iraqi goats. A number of different mutations were found owing to seven main polymorphisms. Results indicated the occurrence of silent and missense mutations in sequences obtained for Iraqi local goats. Genetic diversity was observed in the HSP70 gene of Iraqi local goats on the basis of phylogenetic-tree analysis as some mutations occurred once whereas others occurred multiple times. The polymorphisms LC616787, LC616788, and LC616791 were combined with the reference gene in the same branch, whereas polymorphisms (LC616785 and LC616786) and (LC616789 and LC616790) met in different branches, respectively. Moreover, all studied proteins had mismatches in their threedimensional structures. Therefore, the presence of specific genetic differences within the HSP70 gene in Iraqi goats can increase the possibility of selecting animals more suitable to various levels of stress.

show abstract

Molecular characterization of heat shock protein 70 gene in Iraqi buffalo

Cited by 6 publications

References 18 publications

GnRHR gene polymorphism and its correlation with semen quality in Buffalo bulls (Bubalus bubalis)

GnRHR gene polymorphism and its correlation with semen quality in Buffalo bulls (Bubalus bubalis)

Physiological and Pyhsical Responses of Dairy Cattle to Heat Stress

Genetic profiling of HSP70 gene in local Iraqi goats

Contact Info

Product

Resources

About