Molecular characterization of Helicobacter pylori strains isolated from cynomolgus monkeys (M. fascicularis)

Doi, Sonia Q.; Kimbason, Tara; Reindel, J. F.; Dubois, A.

doi:10.1016/j.vetmic.2005.04.003

Cited by 13 publications

(10 citation statements)

References 20 publications

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“…However, the isolation of the H. suis HS1 strain from the gastric mucosa of an infected swine [ 64 ] invokes more speculative models of tfs3 ICE acquisition, requiring either transient infection of the source animal with H. pylori , or human to animal (re)transmission of the HS1 strain. These models of anthroponotic infection remain unexplored, although are plausible as H. pylori has proven capacity to infect other animal species [ 75 , 76 ] and is the likely ancestral origin of H. acinonychis [ 18 ]. Important clarity is therefore required regarding contemporary tfs3 gene flow between H. pylori and H. suis , since a human adapted H. pylori tfs3 could conceivably confer attributes which influence colonisation and/or virulence by H. suis within a human or porcine host.…”

Section: Discussionmentioning

confidence: 99%

Phylogeographic diversity and mosaicism of the Helicobacter pylori tfs integrative and conjugative elements

2018

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BackgroundThe genome of the gastric pathogen Helicobacter pylori is characterised by considerable variation of both gene sequence and content, much of which is contained within three large genomic islands comprising the cag pathogenicity island (cagPAI) and two mobile integrative and conjugative elements (ICEs) termed tfs3 and tfs4. All three islands are implicated as virulence factors, although whereas the cagPAI is well characterised, understanding of how the tfs elements influence H. pylori interactions with different human hosts is significantly confounded by limited definition of their distribution, diversity and structural representation in the global H. pylori population.ResultsTo gain a global perspective of tfs ICE population dynamics we established a bioinformatics workflow to extract and precisely define the full tfs pan-gene content contained within a global collection of 221 draft and complete H. pylori genome sequences. Complete (ca. 35-55kbp) and remnant tfs ICE clusters were reconstructed from a dataset comprising > 12,000 genes, from which orthologous gene complements and distinct alleles descriptive of different tfs ICE types were defined and classified in comparative analyses. The genetic variation within defined ICE modular segments was subsequently used to provide a complete description of tfs ICE diversity and a comprehensive assessment of their phylogeographic context. Our further examination of the apparent ICE modular types identified an ancient and complex history of ICE residence, mobility and interaction within particular H. pylori phylogeographic lineages and further, provided evidence of both contemporary inter-lineage and inter-species ICE transfer and displacement.ConclusionsOur collective results establish a clear view of tfs ICE diversity and phylogeographic representation in the global H. pylori population, and provide a robust contextual framework for elucidating the functional role of the tfs ICEs particularly as it relates to the risk of gastric disease associated with different tfs ICE genotypes.Electronic supplementary materialThe online version of this article (10.1186/s13100-018-0109-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Phylogeographic diversity and mosaicism of the Helicobacter pylori tfs integrative and conjugative elements

2018

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“…Within this sub-domain, it was possible to design an ISH probe and a set of real-time RT-PCR primers and a TaqMan probe that are 100% homologous with over 100 H. pylori strains isolated from humans residing in four continents, from monkeys [14], [32], and from cats [33]. In addition, 100% homology was found with many Helicobacter sp.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, we used an in silico approach to demonstrate that a 546-bp domain of H. pylori 16S rRNA is highly conserved in most H. pylori 16S rRNA sequences registered in the NCBI GenBank and that a 229-bp sub-domain of this conserved region is specific to H. pylori . Within this sub-domain, it was possible to design an ISH probe and a set of real-time RT-PCR primers and a TaqMan probe that are 100% homologous with over 100 H. pylori strains isolated from humans residing in four continents, from monkeys [14] , [32] , and from cats [33] . In addition, 100% homology was found with many Helicobacter sp.…”

Section: Discussionmentioning

confidence: 99%

Specific and Sensitive Detection of H. pylori in Biological Specimens by Real-Time RT-PCR and In Situ Hybridization

et al. 2008

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PCR detection of H. pylori in biological specimens is rendered difficult by the extensive polymorphism of H. pylori genes and the suppressed expression of some genes in many strains. The goal of the present study was to (1) define a domain of the 16S rRNA sequence that is both highly conserved among H. pylori strains and also specific to the species, and (2) to develop and validate specific and sensitive molecular methods for the detection of H. pylori. We used a combination of in silico and molecular approaches to achieve sensitive and specific detection of H. pylori in biologic media. We sequenced two isolates from patients living in different continents and demonstrated that a 546-bp domain of the H. pylori 16S rRNA sequence was conserved in those strains and in published sequences. Within this conserved sequence, we defined a 229-bp domain that is 100% homologous in most H. pylori strains available in GenBank and also is specific for H. pylori. This sub-domain was then used to design (1) a set of high quality RT-PCR primers and probe that encompassed a 76-bp sequence and included at least two mismatches with other Helicobacter sp. 16S rRNA; and (2) in situ hybridization antisense probes. The sensitivity and specificity of the approaches were then demonstrated by using gastric biopsy specimens from patients and rhesus monkeys. This H. pylori-specific region of the 16S rRNA sequence is highly conserved among most H. pylori strains and allows specific detection, identification, and quantification of this bacterium in biological specimens.

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“…Macaques are naturally infected with several strains closely related to that of humans and are also highly susceptible to the human strain (Doi et al 2005;Dubois et al 1994Dubois et al , 1995Solnick et al 1999Solnick et al , 2003. They develop gastritis and antral erosions indistinguishable from those that occur in humans (Reindel et al 1999;Shuto et al 1993).…”

Section: Gastritis/gastric Cancer (Helicobacter Pylori)mentioning

confidence: 99%

Macaque Models of Human Infectious Disease

Gardner¹,

Luciw²

2008

ILAR Journal

174

133

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Macaques have served as models for more than 70 human infectious diseases of diverse etiologies, including a multitude of agents-bacteria, viruses, fungi, parasites, prions. The remarkable diversity of human infectious diseases that have been modeled in the macaque includes global, childhood, and tropical diseases as well as newly emergent, sexually transmitted, oncogenic, degenerative neurologic, potential bioterrorism, and miscellaneous other diseases. Historically, macaques played a major role in establishing the etiology of yellow fever, polio, and prion diseases. With rare exceptions (Chagas disease, bartonellosis), all of the infectious diseases in this review are of Old World origin. Perhaps most surprising is the large number of tropical (16), newly emergent (7), and bioterrorism diseases (9) that have been modeled in macaques. Many of these human diseases (e.g., AIDS, hepatitis E, bartonellosis) are a consequence of zoonotic infection. However, infectious agents of certain diseases, including measles and tuberculosis, can sometimes go both ways, and thus several human pathogens are threats to nonhuman primates including macaques. Through experimental studies in macaques, researchers have gained insight into pathogenic mechanisms and novel treatment and vaccine approaches for many human infectious diseases, most notably acquired immunodeficiency syndrome (AIDS), which is caused by infection with human immunodeficiency virus (HIV). Other infectious agents for which macaques have been a uniquely valuable resource for biomedical research, and particularly vaccinology, include influenza virus, paramyxoviruses, flaviviruses, arenaviruses, hepatitis E virus, papillomavirus, smallpox virus, Mycobacteria, Bacillus anthracis, Helicobacter pylori, Yersinia pestis, and Plasmodium species. This review summarizes the extensive past and present research on macaque models of human infectious disease.

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Molecular characterization of Helicobacter pylori strains isolated from cynomolgus monkeys (M. fascicularis)

Cited by 13 publications

References 20 publications

Phylogeographic diversity and mosaicism of the Helicobacter pylori tfs integrative and conjugative elements

Phylogeographic diversity and mosaicism of the Helicobacter pylori tfs integrative and conjugative elements

Specific and Sensitive Detection of H. pylori in Biological Specimens by Real-Time RT-PCR and In Situ Hybridization

Macaque Models of Human Infectious Disease

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