Molecular Characterization of High-Level Mupirocin Resistance in Staphylococcus pseudintermedius

Matanović, Krešimir; Pérez-Roth, Eduardo; Pintarić, Selma; Martinec, Branka Šeol

doi:10.1128/jcm.02904-12

Cited by 20 publications

(12 citation statements)

References 16 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This structural group is similar to the structure previously reported for the plasmid-borne ileS2 gene identified in S. pseudintermedius isolated from a dog with pyoderma in Croatia (6). The resultant PCR products were sequenced and compared to the previously published ileS2 sequences from S. pseudintermedius, JX186508 and JX186509 (6). Sequences from this study were deposited in GenBank as KJ000545, KJ000546, and KJ000547.…”

mentioning

confidence: 62%

“…Template DNA used for PCR was plasmid DNA from isolate 39-045 (lanes 2 and 5) or genomic DNA from ATCC 29213 (lanes 3 and 6). Water was substituted for DNA in lanes 4 and 7. published primers (6). The resultant 945-bp product was sequenced and analyzed using MEGA5.1 software, and the sequence was deposited in GenBank as KJ000544.…”

mentioning

confidence: 99%

“…Resultant plasmids containing the upstream IS257-ileS2 junction, the downstream ileS2-IS257 junction, and the 945-bp fragment of the native ileS gene were submitted to the Texas A&M Gene Technologies Laboratory for sequencing. Resultant sequences were compared to sequences in GenBank (JX186508, JX186509, JX186511, JX186512, JX186513, and JX186514) using MEGA5.1 software (6,12).…”

mentioning

confidence: 99%

“…The primers IS257 F , ileS2-5=, ileS2-3=, and IS257 R (Table 1) were used in various combinations as previously described under the following conditions: 94°C for 5 min; 30 cycles of 94°C for 30 s, 60°C for 40 s, and 72°C for 60 s; then 72°C for 10 min; and then a hold at 4°C (11). PCR was performed to identify the native ileS gene using the primers ileS-F1 and ileS-R1 (Table 1) (6). Conditions for the thermal cycler were as follows: 94°C for 5 min; 30 cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 60 s; then 72°C for 7 min; and a hold at 4°C.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Prevalence of Mupirocin Resistance in Staphylococcus pseudintermedius

2014

View full text Add to dashboard Cite

In the United States, veterinary use of mupirocin is primarily limited to the treatment of canine pyoderma caused by methicillinresistant Staphylococcus pseudintermedius (MRSP). In this study, only 1 of 581 S. pseudintermedius isolates tested was resistant to mupirocin and carried the high-level mupirocin resistance gene, ileS2, on a plasmid. Staphylococcus pseudintermedius is the primary bacterial pathogen isolated from canine pyoderma and also causes postsurgical infections in dogs (1, 2). Methicillin resistance and multidrug resistance are increasing in S. pseudintermedius, thus limiting the options for therapeutic treatment of canine skin infections (2). Mupirocin is a bacteriostatic antibiotic that reversibly binds to isoleucyl-tRNA synthetase to disrupt protein synthesis and is widely used to eliminate nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) in human MRSA carriers (3). Mupirocin has been used on only a limited basis in veterinary medicine but is approved in the United States for the treatment of bacterial skin infections and superficial pyoderma in dogs (4).In S. aureus, two levels of mupirocin resistance have been identified. Low-level mupirocin resistance occurs due to a point mutation to the chromosomal ileS gene that encodes the native isoleucyl-tRNA synthetase. The MIC for mupirocin for staphylococci carrying the low-level resistance is Ն8 g/ml but Յ256 g/ml (5). Conversely, high-level mupirocin resistance (MIC of Ն512 g/ml) is usually conferred by the plasmid-borne ileS2, although a chromosomal location of ileS2 has been reported (5). Recently, ileS2 plasmid-mediated mupirocin resistance was found in a mupirocin-resistant, methicillin-susceptible S. pseudintermedius strain isolated from a dog in Croatia (6). The goal of the present study was to determine the prevalence of mupirocin resistance in S. pseudintermedius isolated from patients presented to a veterinary hospital in Texas.In this study, 581 isolates of S. pseudintermedius were screened for phenotypic low-level mupirocin resistance. Isolates were collected from veterinary patients, predominantly dogs (n ϭ 446), but also included isolates from cats (n ϭ 9). Some patients were cultured at multiple sites and contributed more than one isolate, and of these, 21 patients contributed more than two isolates. The isolates included a historical collection of 403 isolates from clinical infections and contained both methicillin-resistant S. pseudintermedius (MRSP) isolates (n ϭ 153) and methicillin-susceptible S. pseudintermedius (MSSP) isolates (n ϭ 250). The isolates from clinical infections were collected from the following anatomic sites: skin (n ϭ 96), external ear canal (n ϭ 31), wounds (n ϭ 79), postoperative infections (n ϭ 33), urine or the urinary tract (n ϭ 87), and other sources (n ϭ 77). Additional isolates were collected during a study of MRSP prevalence in canine patients without clinical staphylococcal infection that presented for elective orthopedic procedures. The MRSP prevalence study yielded 178 S. pseudin...

show abstract

mentioning

confidence: 62%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Prevalence of Mupirocin Resistance in Staphylococcus pseudintermedius

2014

View full text Add to dashboard Cite

show abstract

“…The genus Staphylococcus comprises Gram‐positive bacteria that are normal skin flora in humans and animals . Some species serve as opportunistic pathogens; for example, Staphylococcus pseudintermedius is the primary cause of canine pyoderma . The emergence of meticillin‐resistant S. pseudintermedius (MRSP) is a major concern in veterinary medicine .…”

Section: Introductionmentioning

confidence: 99%

Low prevalence of mupirocin resistance in Staphylococcus pseudintermedius isolates from canine pyoderma in Korea

Park

Kang

Hyun

et al. 2018

Veterinary Dermatology

View full text Add to dashboard Cite

show abstract

Diagnostic microbiology in veterinary dermatology: present and future

Guardabassi

Damborg

Stamm

et al. 2017

Advances in Veterinary Dermatology

View full text Add to dashboard Cite

Background -The microbiology laboratory can be perceived as a service provider rather than an integral part of the healthcare team.Objectives -The aim of this review is to discuss the current challenges of providing a state-of-the-art diagnostic veterinary microbiology service including the identification (ID) and antimicrobial susceptibility testing (AST) of key pathogens in veterinary dermatology.Methods -The Study Group for Veterinary Microbiology (ESGVM) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) identified scientific, technological, educational and regulatory issues impacting the predictive value of AST and the quality of the service offered by microbiology laboratories.Results -The advent of mass spectrometry has significantly reduced the time required for ID of key pathogens such as Staphylococcus pseudintermedius. However, the turnaround time for validated AST methods has remained unchanged for many years. Beyond scientific and technological constraints, AST methods are not harmonized and clinical breakpoints for some antimicrobial drugs are either missing or inadequate. Small laboratories, including in-clinic laboratories, are usually not adequately equipped to run up-to-date clinical microbiologic diagnostic tests.Conclusions and clinical importance -ESGVM recommends the use of laboratories employing mass spectrometry for ID and broth micro-dilution for AST, and offering assistance by expert microbiologists on pre-and post-analytical issues. Setting general standards for veterinary clinical microbiology, promoting antimicrobial stewardship, and the development of new, validated and rapid diagnostic methods, especially for AST, are among the missions of ESGVM.

show abstract

Molecular Characterization of High-Level Mupirocin Resistance in Staphylococcus pseudintermedius

Cited by 20 publications

References 16 publications

Prevalence of Mupirocin Resistance in Staphylococcus pseudintermedius

Prevalence of Mupirocin Resistance in Staphylococcus pseudintermedius

Low prevalence of mupirocin resistance in Staphylococcus pseudintermedius isolates from canine pyoderma in Korea

Diagnostic microbiology in veterinary dermatology: present and future

Contact Info

Product

Resources

About