Molecular characterization of high-risk humanpapillomavirus genotypes in women with or without cervical lesions at VIA/VILI in Kara, Togo

Dolou, Essolakina Dolou; Kuassi-Kpede, Akouélé P.; Zohoncon, Théodora Mahoukèdè; Traoré, Issouf; Katawa, Gnatoulma; Ouedraogo, Rogomenoma Alice; Traoré, Esther Mah Alima; Bado, Prosper; Ouédraogo, C.M.R.; Djigma, Florencia Wendkuuni; Aboubakari, Abdoul‐Samadou; Karou, Simplice Damintoti; Simporé, Jacques

doi:10.4314/ahs.v21i4.27

Cited by 4 publications

(7 citation statements)

References 15 publications

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“…This implies that HPV genotype distribution is diverse at different places and periods even within the same population [54]. Similarly, the distribution of HR-HPVs among women in other African countries was reported to heterogeneous [25,49,52,55,56].…”

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

“…For instance, HPV16 is not the first-ranked genotype from cervical samples in Kenya [53], Togo [51,56], and Nigeria [22]. According to studies, not only HPV16 but also HPV18 is not that important in some African countries [51,56]. However, systematic reviews in Africa, Asia, Latin America, and North America consistently reported that HPV16 is the most frequently identified genotype among women with various degrees of cervical lesions [57][58][59][60].…”

Section: Discussionmentioning

confidence: 99%

“…The time to the first result is less than two hours [43]. Using real-time PCR and ReadiFlex ® technology, the Alinity m HR HPV assay is a qualitative in vitro test targeting the conserved L1 region for the detection of DNA from 14 HR-PV genotypes; HPV16, 18,31,33,35,39,45,51,52,56,58,59, 66, and 68 from clinical specimen [42]. HR-HPVs were detected with genotype-specific probes in five distinct channels: HPV16, HPV18, HPV45, and other high-risk genotypes group A (HPV31, 33, 52, and 58), and other high-risk genotypes group B (HPV35, 39, 51, 56, 59, 66, and 68) at clinically relevant infection levels [41].…”

Section: Hpv Dna Detection and Genotypingmentioning

confidence: 99%

“…Of all types, about 40 are sexually transmitted [8,[11][12][13][14]. HPV16, 18,31,33,35,39,45,51,52,56,58,59 and 68 grouped under HR-HPV because of their strong association in carcinogenesis. Infection with HPV type 16 or HPV18 is associated with a higher risk of disease progression compared to other HR-HPVs causing more than 50% of all cervical cancer burdens globally [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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High-risk human papillomavirus genotype distribution among women with gynecology complaints in northwest Ethiopia

Derbie

Maier

Amare

et al. 2023

Infect Agents Cancer

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Background Human papillomavirus (HPV) genotypes differ by geographic location. With the advent of HPV vaccination and HPV-based cervical screening tests in Ethiopia, a nationwide dataset on the genotype distribution of HPV among women has paramount importance in the fight against cervical cancer. However, there is limited data in this regard in the northwest part of the country. Therefore, this study aimed to identify the genotype distribution of high-risk HPVs among women presenting with cervical abnormalities. Methods A health facility-based cross-sectional study was conducted at Felege Hiwot Comprehensive Specialized Hospital (FHCSH), Bahir Dar–Ethiopia. Women aged ≥ 30 years who visited the hospital gynecology unit from 01 March 2019 to 30 October 2021 were included. Following general and pelvic examinations, a senior gynecologist collected cervical punch biopsies for histopathological examinations and cervical swabs for HR-HPV detection using the Abbott Alinity m system (Abbott Molecular, Des Plaines, IL, USA). Extended genotyping was carried out with the INNO-LiPA HPV Genotyping Extra II assay (INNO-LiPA; Fujirebio Europe, Ghent, Belgium) as per the manufacturer protocols at the Institute of Virology, Leipzig University Hospital, Germany. Results We included 355 women with a mean age of 46.4 ± 11.4 years. The majority of the participants, 277 (79.4%) were sexually active before the age of 18 years and 180 (51.6%) had multiple sexual partners. Forty-eight (13.5%) of the participants were HIV positive. The proportion of HR-HPV was 53.0% (n = 188; 95%CI: 47.8–58.1%). From these samples, 13 different HR-HPV types with a total of 258 sequences were identified. The detection of HR-HPV increased significantly with an increase in the age of the participants. The predominant identified HR-HPV was HPV16, 50.4% followed by HPV31 (9.7%), HPV33 (8.5%), HPV39, and HPV68 each (5.8%) and HPV18 (4.7%). Of the total HR-HPV-positive women, 23.9% (45/188) were infected with multiple HR-HPV types. All HPV16, HPV18, HPV35, and HPV45 genotypes (as a single or in coinfections) were found to be associated with either high-grade lesions or cervical cancer. Conclusions HR-HPV infection was reportedly higher among women in the present study area. Based on our findings, we strongly recommend the nonavalent HPV vaccine for immunization and any HPV-based screening method to take into consideration the predominant genotypes circulating in the country. The role of multiple HPV infections in high-grade cervical lesions entails further study in Ethiopia.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Hpv Dna Detection and Genotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-risk human papillomavirus genotype distribution among women with gynecology complaints in northwest Ethiopia

Derbie

Maier

Amare

et al. 2023

Infect Agents Cancer

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Distribution of high- and low-risk human papillomavirus genotypes and their prophylactic vaccination coverage among West African women: systematic review

Ouedraogo,

Kande,

Nadembega

et al. 2023

J Egypt Natl Canc Inst

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Introduction The second most deadly gynecological cancer worldwide, cervical cancer is steadily on the rise in sub-Saharan Africa, while vaccination programs are struggling to get off the ground. This systematic review’s aim was to assess the prevalence and distribution of high- and low-risk HPV genotypes in West African women. Methods Original studies were retrieved from PubMed/Medline, Embase, Scopus, Google Scholar, and Science Direct. In these studies, Human papillomavirus (HPV) DNA was assessed in cervical samples by polymerase chain reaction (PCR), Hybrid capture, and sequencing. The quality of the articles was assessed and the results were extracted and reviewed. Results Thirty-nine studies from 10 West African countries were included for the systematic review including 30 for the pooled analysis. From an overall of 17358 participants, 5126 of whom were infected with at least one HPV genotype, the systematic review showed a prevalence varying from 8.9% to 81.8% in the general population. In contrast, the pooled prevalence of infection was 28.6% (n = 3890; 95% CI 27.85–29.38), and HPV-52 (13.3%), HPV-56 (9.3%), and HPV-35 (8.2) were the most frequent. Quadrivalent and nonavalent vaccines covered 18.2% and 55.8% of identified genotypes respectively. Conclusion Faced with this growing public health challenge in West Africa, it would be necessary for all its countries to have reliable data on HPV infection and to introduce the nonavalent vaccine. A study of the genotypic distribution of HPV in high-grade precancerous lesions and cervical cancer would be very useful in West Africa.

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Prevalence and Genotype Distribution of High-Risk Human Papillomavirus Infection Among Sub-Saharan African Women: A Systematic Review and Meta-Analysis

Seyoum

Assefa

Gure

et al. 2022

Front. Public Health

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BackgroundAmong sub-Saharan African women, cervical cancer is steadily increasing with more than 75,000 new cases and 50,000 deaths annually. Due to the vast ethno geography variation, Africa harbors heterogeneous genotypes of HPV. High-risk HPV [hr HPV] genotypes such as hr HPV-16,−18,-35, and−52 are abundantly reported in sub-Saharan Africa. The purpose of this systematic review and meta-analysis is to generate an evidence on the prevalence and the genotype distribution of hr HPV among sub-Saharan African countries.MethodsThe review was conducted by following the preferred reporting items for systematic reviews and Meta-analysis. PubMed/Medline, Embase, Scopus, Google Scholar, Heath Technology assessment and Cochrane Library databases were used to retrieve published original studies between 2001 and 2021. It included studies that used PCR-based or hybrid testing to assess the presence of HPV DNA in a cervical biopsy, cervical swelling, and vaginal swelling. Statistical software for data science (STATA V16) software using a random-effects model was used to determine the pooled prevalence and type-specific distribution of HPV with 95% confidence intervals (CI). The I-squared statistic was used to describe the level of heterogeneity. The study protocol is registered on PROSPERO with reference number CRD42022311157.ResultsThe review included 27 studies conducted in 19 sub-Saharan countries. A total of 16,506 study participants from 27 studies were included in a systematic review and 5,303 of them were infected with the hr HPV infection. Out these, only 3,075 of them were eligible for meta-analysis. The incidence proportion of estimatesof hr HPV infection among study participants with different health conditions ranges from 10.7 to 97.2% while the pooled incidence proportion of estimates is 34% (95%CI: 29–39). Among 3,075 women, 424 (13.8%), 305 (9.9%) and 279 (9%) were infected with HPV-16,−52 and−18, respectively. HPV-16 and−52 are the main genotypes causing the hr HPV infection in the Eastern and Southern African sub-contents, whereas HPV-16 and−35 are the main genotypes in the Western African countries.ConclusionsDepending on several factors, especially women's health conditions, the high rate of hr HPV infection with inconsistent genotype distribution shows that it is a growing public health challenge in sub-Saharan African countries. Therefore, to implement a vaccination-based prevention strategy and be effective, considering factors associated with hr HPV infection is crucial.

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Molecular characterization of high-risk humanpapillomavirus genotypes in women with or without cervical lesions at VIA/VILI in Kara, Togo

Cited by 4 publications

References 15 publications

High-risk human papillomavirus genotype distribution among women with gynecology complaints in northwest Ethiopia

High-risk human papillomavirus genotype distribution among women with gynecology complaints in northwest Ethiopia

Distribution of high- and low-risk human papillomavirus genotypes and their prophylactic vaccination coverage among West African women: systematic review

Prevalence and Genotype Distribution of High-Risk Human Papillomavirus Infection Among Sub-Saharan African Women: A Systematic Review and Meta-Analysis

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