Abstract:The 18S rDNA gene analysis with Internal Transcribed Spacers 1 and 2, and Mit. coi gene analyses of 12 sequences from the host-specific nematodes parasitizing liver of garfish, Xenentodon cancila (Belonidae) revealed their monophyletic characterization. The purification of PCR products was done using Centri-Sep spin columns (Princeton Separations, Adelphia, New Jersey, USA) and sequenced by ABI 3730 auto sequencer Applied Biosystems. Their genetic heterogeneity on the basis of comparable sequences from gnathos… Show more
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