Molecular Characterization of Human Clinical Isolates of<i>Yersinia enterocolitica</i>Bioserotype 1B/O8 in Poland: Emergence and Dissemination of Three Highly Related Clones

Gierczyński, Rafał; Szych, J; Rastawicki, Waldemar; Wardak, Sebastian; Jagielski, M

doi:10.1128/jcm.01321-08

Cited by 11 publications

(5 citation statements)

References 20 publications

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“…Biotype 1A isolates are considered nonpathogenic, which is concordant with a lack of the major Y. enterocolitica virulence factors such as pYV, invasin, YadA, and Ail (5), although there are numerous reports of biotype 1A human carriage (6,7). Biotype 1B isolates are high pathogenic, which is concordant with carriage of the high-pathogenicity island, but isolation from human disease cases is very rare with the exception of notable outbreaks such as the recent emergence in Poland (8). Biotype 2 to 4 isolates are low pathogenic and are globally the most common causes of human gastrointestinal yersiniosis (4).…”

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confidence: 97%

Use of Whole-Genus Genome Sequence Data To Develop a Multilocus Sequence Typing Tool That Accurately Identifies Yersinia Isolates to the Species and Subspecies Levels

et al. 2015

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hThe genus Yersinia is a large and diverse bacterial genus consisting of human-pathogenic species, a fish-pathogenic species, and a large number of environmental species. Recently, the phylogenetic and population structure of the entire genus was elucidated through the genome sequence data of 241 strains encompassing every known species in the genus. Here we report the mining of this enormous data set to create a multilocus sequence typing-based scheme that can identify Yersinia strains to the species level to a level of resolution equal to that for whole-genome sequencing. Our assay is designed to be able to accurately subtype the important human-pathogenic species Yersinia enterocolitica to whole-genome resolution levels. We also report the validation of the scheme on 386 strains from reference laboratory collections across Europe. We propose that the scheme is an important molecular typing system to allow accurate and reproducible identification of Yersinia isolates to the species level, a process often inconsistent in nonspecialist laboratories. Additionally, our assay is the most phylogenetically informative typing scheme available for Y. enterocolitica. The Gram-negative Yersinia is one of the most important and well-studied bacterial genera, consisting of three human pathogens. Y. pestis is the causative agent of bubonic and pneumonic plague and is a recently diverged clone of Yersinia pseudotuberculosis (1), which alongside Y. enterocolitica is a zoonotic gastrointestinal pathogen (2). The remaining species are not associated with human disease and are considered to be environmental organisms, with the exception of the common fish pathogen Y. ruckeri (2) and the insecticidal species Y. entomophaga. Of the human-pathogenic species, Y. enterocolitica is the most common etiological agent of human disease, and in Germany and Scandinavia, the numbers of cases of human intestinal yersiniosis caused by Y. enterocolitica rival those caused by Salmonella (3). Y. enterocolitica is in itself a very diverse species that is classically subdivided into nonpathogenic, low-pathogenic, and high-pathogenic biotypes based on virulence in a mouse infection model (4). Biotype 1A isolates are considered nonpathogenic, which is concordant with a lack of the major Y. enterocolitica virulence factors such as pYV, invasin, YadA, and Ail (5), although there are numerous reports of biotype 1A human carriage (6, 7). Biotype 1B isolates are high pathogenic, which is concordant with carriage of the high-pathogenicity island, but isolation from human disease cases is very rare with the exception of notable outbreaks such as the recent emergence in Poland (8). Biotype 2 to 4 isolates are low pathogenic and are globally the most common causes of human gastrointestinal yersiniosis (4). Biotype 5 isolates are also considered low pathogenic but have only been isolated from wild hare populations and are very rare in nature (5).From a clinical perspective, the isolation and subsequent identification of Yersinia and in particular Y. enterocoli...

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confidence: 97%

Use of Whole-Genus Genome Sequence Data To Develop a Multilocus Sequence Typing Tool That Accurately Identifies Yersinia Isolates to the Species and Subspecies Levels

et al. 2015

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“…Biotype 1A is deemed to be non-or less pathogenic for humans. Biotype 1B is widespread in the United States and only rarely detected in Europe and Japan (11,14,26,28). Based on different DNA-DNA hybridization values and 16S rRNA gene sequences, it was proposed to name the "American" strains Y. enterocolitica subsp.…”

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confidence: 99%

Identification ofYersinia enterocoliticaat the Species and Subspecies Levels by Fourier Transform Infrared Spectroscopy

Kuhm

Suter

Felleisen

et al. 2009

Appl Environ Microbiol

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intermedia, and Y. rohdei strains from Y. enterocolitica, which could not be differentiated by the API 20E test system. The probability for correct biotype identification of Y. enterocolitica isolates was 98.3% (41 externally validated strains). For correct serotype identification, the probability was 92.5% (42 externally validated strains). In addition, the presence or absence of the ail gene, one of the main pathogenicity markers, was demonstrated using FT-IR. The probability for correct identification of isolates concerning the ail gene was 98.5% (51 externally validated strains). This indicates that it is possible to obtain information about genus, species, and in the case of Y. enterocolitica also subspecies type with a single measurement. Furthermore, this is the first example of the identification of specific pathogenicity using FT-IR.

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“…In a previous study, we showed that clinical isolates of Y. enterocolitica 1B/O8 collected in Poland were clonal and belonged to the same strain sensu stricto [22]. This strong clonality of Polish Y. enterocolitica 1B/O :8 isolates may suggest a common origin.…”

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confidence: 85%

Seasonality ofYersinia enterocoliticabioserotype 1B/O:8 infections in Poland

et al. 2012

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Both serological and bacteriological investigations revealed a cyclic, seasonal pattern of Yersinia enterocolitica 1B/O8 infections in Poland during the years 2008–2011. A large increase in incidence was observed in the second quarter and a decrease in the third quarter of each year. Such seasonal changes were not seen in the case of infections caused by the other enteropathogenic Yersinia bioserotypes.

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Molecular Characterization of Human Clinical Isolates ofYersinia enterocoliticaBioserotype 1B/O8 in Poland: Emergence and Dissemination of Three Highly Related Clones

Cited by 11 publications

References 20 publications

Use of Whole-Genus Genome Sequence Data To Develop a Multilocus Sequence Typing Tool That Accurately Identifies Yersinia Isolates to the Species and Subspecies Levels

Use of Whole-Genus Genome Sequence Data To Develop a Multilocus Sequence Typing Tool That Accurately Identifies Yersinia Isolates to the Species and Subspecies Levels

Identification ofYersinia enterocoliticaat the Species and Subspecies Levels by Fourier Transform Infrared Spectroscopy

Seasonality ofYersinia enterocoliticabioserotype 1B/O:8 infections in Poland

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